BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0064.p (511 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 999 0.0 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 401 e-113 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 326 3e-90 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 282 4e-77 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 206 5e-54 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 999 bits (2583), Expect = 0.0, Method: Compositional matrix adjust. Identities = 506/507 (99%), Positives = 506/507 (99%) Query: 5 MTQQDLFKLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVAD 64 MTQQDLFKLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVAD Sbjct: 1 MTQQDLFKLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVAD 60 Query: 65 GKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRG 124 GKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRG Sbjct: 61 GKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRG 120 Query: 125 SSGSAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLA 184 SSGSAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLA Sbjct: 121 SSGSAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLA 180 Query: 185 AARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQT 244 AARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQT Sbjct: 181 AARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQT 240 Query: 245 QLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERR 304 QLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERR Sbjct: 241 QLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERR 300 Query: 305 PDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQ 364 PDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQ Sbjct: 301 PDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQ 360 Query: 365 TIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQA 424 TIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQA Sbjct: 361 TIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQA 420 Query: 425 ARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDE 484 ARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDE Sbjct: 421 ARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDE 480 Query: 485 HSLDDLGSLQQQSAEKAKALKENAALR 511 SLDDLGSLQQQSAEKAKALKENAALR Sbjct: 481 RSLDDLGSLQQQSAEKAKALKENAALR 507 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 401 bits (1030), Expect = e-113, Method: Compositional matrix adjust. Identities = 247/484 (51%), Positives = 327/484 (67%), Gaps = 28/484 (5%) Query: 14 KPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEMFGD 73 K + A+++A+L L C+VGPDY RP +D P +KE WK A PR + WWE +GD Sbjct: 33 KGMTALVLAAL-LGGCAVGPDYARPALDL-PQAYKETGPWKLAAPRQIDDHQPWWEAYGD 90 Query: 74 PQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAGASS 133 P LNAL+ Q + +NQ + A A +R A A + +R++ PT+ A AGASR + + G Sbjct: 91 PVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTNGVQR 150 Query: 134 SNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQ 193 + NSY+ L A+WEAD+WG++RR E+ +A+AQASAA LAAARLS QA Sbjct: 151 -----------LGNSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQAT 199 Query: 194 VAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQASA 253 +AQ+YLQLRV D Q+ L TV AY RSLQ++ +QY AG +SDV QA+TQL++AQA Sbjct: 200 LAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQL 259 Query: 254 LDNGVTRAQVEHAIALLVGKTPAQFALPRAP---------------LAAVAPPVPQGVPS 298 +D TR Q+EHAIA+L+GK PAQF+LP P L P +P G+PS Sbjct: 260 IDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSLPQIPVGLPS 319 Query: 299 MLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSL 358 LLERRPD+A AER A+ANA IGVA+AAYFP+LTLSASGGY S FA+ F P RVWSL Sbjct: 320 ELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSL 379 Query: 359 GPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQ 418 G LA+++FDGGAR+A D A+A YD VA YKQTVL QEVED L+ LR+L+QE+ VQ Sbjct: 380 GSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQ 439 Query: 419 GEALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKAL 478 +A+Q+A+ A L QY+AGTV+Y++V+T QA +L+ + +++ +L R++ ASV L KA Sbjct: 440 AQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKAT 499 Query: 479 GGGW 482 GGGW Sbjct: 500 GGGW 503 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 326 bits (835), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 201/468 (42%), Positives = 282/468 (60%), Gaps = 19/468 (4%) Query: 29 CSVGPDYVRPQMDTGPAIFKE----------GQNWKPAQPRDLVADGKWWEMFGDPQLNA 78 CSV P Y RP +DT PA FKE G WK AQP + +A G+WW++FGD +LNA Sbjct: 31 CSVAPTYERPSVDT-PAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 79 LVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAGASSSNAAS 138 L +NQ+L A A A AL + +R+ Y P + A GA+R A S Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPA----SQGLP 145 Query: 139 GSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQVAQNY 198 + Y + ++E D++G+V V+++ A AQ S A + L+ QA VAQ Y Sbjct: 146 ADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQY 205 Query: 199 LQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQASALDNGV 258 +R LDA L TV + SL++ Q ++ G +++ DV +A+ +L SAQ+ AL Sbjct: 206 FLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIAR 265 Query: 259 TRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERRPDVAAAERRVASAN 318 RA EHA+A+L+G+TPA+F+LP PL +A VP G+PS LLERRPD+AAAER +A+AN Sbjct: 266 QRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAAAERAMAAAN 325 Query: 319 AKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGP----QLAQTIFDGGARAA 374 A++GVAK+A+FPSL L+ GY+S+ D F R + LGP L+ +FDGG R A Sbjct: 326 ARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQA 385 Query: 375 ATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAVTLVNN 434 D+A A+Y+ VA+Y+ TVL AF+EVEDNLA LRIL+Q+ Q EA+ A+ +A L + Sbjct: 386 GVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHT 445 Query: 435 QYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGW 482 QY+ G VSY +VI A T L + +S+ + R ++V L +ALGGGW Sbjct: 446 QYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 282 bits (722), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 185/459 (40%), Positives = 270/459 (58%), Gaps = 5/459 (1%) Query: 29 CSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEMFGDPQLNALVAQVDISNQ 88 C+ GPDY RPQ+D PA + W+ QP D G WWE FGD QLNAL Q NQ Sbjct: 19 CASGPDYTRPQVDM-PAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 89 NLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAGASSSNAASGSSAGSVRNS 148 LA+A A A A + + +A +P ++ +A A+R S SA +N S + +V+N Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAAR-SRISANRPLTNYNS-PNFSTVQND 135 Query: 149 YTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQVAQNYLQLRVLDAQQ 208 + + ++E D +G+V+R VE++ A+A+ SAADL RL A++A NY LR LD + Sbjct: 136 FALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVEL 195 Query: 209 QLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQASALDNGVTRAQVEHAIA 268 ++ + ++L++A ++ G + D+ Q Q L S G RAQ EHAIA Sbjct: 196 DVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIA 255 Query: 269 LLVGKTPAQFALPRAPLAAVAPP-VPQGVPSMLLERRPDVAAAERRVASANAKIGVAKAA 327 L G F++ + L + PP +P GVPS +LERRPD+AAAER +A+ANA+IGVA AA Sbjct: 256 TLTGTPAPAFSITPS-LTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAA 314 Query: 328 YFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIATYDGTV 387 Y+PS L S G S +A F P +WSLG +Q++FDGG A D + A Y+ TV Sbjct: 315 YYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATV 374 Query: 388 ATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAVTLVNNQYKAGTVSYVNVI 447 A+Y++TVLTA QEVED + L LE+ A A+ +AR+ + + +++Y+ G Y++VI Sbjct: 375 ASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVI 434 Query: 448 TAQATALSAELSSLNILNRRMAASVLLAKALGGGWDEHS 486 TAQ + L++E + ++ +R+ SV L KALGG W + Sbjct: 435 TAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRT 473 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 206 bits (523), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 158/482 (32%), Positives = 249/482 (51%), Gaps = 21/482 (4%) Query: 12 KLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEMF 71 +L + A LV + L CS+ P Y RPQ + + Q +L W + F Sbjct: 11 RLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNL----GWRQFF 66 Query: 72 GDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAGA 131 D +L L+A +N++L A N A A Q +R+ P L+ S A+R Sbjct: 67 PDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAAR-------- 118 Query: 132 SSSNAASGSSAG--SVRNSYTFSL-TAAWEADIWGKVRRDVESSQASAQASAADLAAARL 188 + AAS S G ++ NSY L A+E D +G+VR +++ AS ++ +A++ Sbjct: 119 -TRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQI 177 Query: 189 SAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKS 248 S AQVAQ YL R Q +L +ET+++ + +L +AQ + G D QT ++S Sbjct: 178 SLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVES 237 Query: 249 AQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAA---VAPPVPQGVPSMLLERRP 305 A+ + RAQ E+A+ LLVGK + LP A L + + +P G+PS LL RP Sbjct: 238 ARVAKTQLERQRAQAENALTLLVGKKIGE--LPPAQLLSDEKIVTDIPAGLPSDLLTNRP 295 Query: 306 DVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQT 365 D+ +AE+ + +ANA IG A+AA+FP ++L+ + G SST F WS GP L+ Sbjct: 296 DIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLP 355 Query: 366 IFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAA 425 IFD G +A D A + V TY++ + TAF+EV D L A L+++ Q L A Sbjct: 356 IFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQ 415 Query: 426 RQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDEH 485 ++ + L + ++K G S ++ + AQ SA+ + + R+ ++ L KALGGG +E+ Sbjct: 416 QERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNEN 475 Query: 486 SL 487 ++ Sbjct: 476 TV 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.123 0.337 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,106,331 Number of extensions: 35352 Number of successful extensions: 244 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 18 Length of query: 511 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 422 Effective length of database: 1,198,455 Effective search space: 505748010 Effective search space used: 505748010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)