BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0061.p (516 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 912 0.0 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 265 6e-72 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 155 1e-38 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 134 2e-32 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 128 1e-30 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 912 bits (2356), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/461 (99%), Positives = 458/461 (99%) Query: 53 MAAMGGPGGRPGPGAAGARRPGGPGAFGSQTMPVGVAKARLQDVNVFLNGLGAVTPTATA 112 MAAMGGPGGRPGPGAAGARRPGGPGAFG QTMPVGVAKARLQDVNVFLNGLGAVTPTATA Sbjct: 1 MAAMGGPGGRPGPGAAGARRPGGPGAFGPQTMPVGVAKARLQDVNVFLNGLGAVTPTATA 60 Query: 113 TVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDL 172 TVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDL Sbjct: 61 TVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDL 120 Query: 173 KRYQTLLAQDSIASQQVDTQAALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGL 232 KRYQTLLAQDSIASQQVDTQ ALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGL Sbjct: 121 KRYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGL 180 Query: 233 RQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDREQK 292 RQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDREQK Sbjct: 181 RQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDREQK 240 Query: 293 NKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTAA 352 NKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTAA Sbjct: 241 NKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTAA 300 Query: 353 IQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREGAKV 412 IQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREGAKV Sbjct: 301 IQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREGAKV 360 Query: 413 EAVARGGADDPANKLTIENPERRHGKRGQGNPGAQAGAGGDAGQGAQGGMSPEERQKRWA 472 EAVARGGADDPANKLT ENPERRHGKRGQGNPGAQAGAGGDAGQGAQGGMSPEERQKRWA Sbjct: 361 EAVARGGADDPANKLTTENPERRHGKRGQGNPGAQAGAGGDAGQGAQGGMSPEERQKRWA 420 Query: 473 ELNKRIDAGEFGEEIKKLPEDQRRQKMMELRRQREAAGNGN 513 ELNKRIDAGEFGEEIKKLPEDQRRQKMMELRRQREAAGNGN Sbjct: 421 ELNKRIDAGEFGEEIKKLPEDQRRQKMMELRRQREAAGNGN 461 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 265 bits (678), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 155/334 (46%), Positives = 220/334 (65%), Gaps = 7/334 (2%) Query: 85 PVGVAKARLQ--DVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEI 142 PV V A +Q D+ +L G+G VT A+ V+ R+DGQL K+ ++EG+ VK G LLA+I Sbjct: 48 PVSVTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQI 107 Query: 143 DPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIASQQVDTQAALVKQYEGT 202 D R+LQA L QAE Q A+D A L +ARLDL+RY TL +QD+ QQ+DTQ ALV Q E Sbjct: 108 DARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAA 167 Query: 203 VKADEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIF 262 VK DE VA A++QLS+T + APISGR+G R D GN V +D NGLV+I Q+ PIT +F Sbjct: 168 VKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVF 227 Query: 263 SIPEDNIPKVLQQLQSGRKLPAQ--AWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQF 320 ++PE+ + + + Q + P Q A+ R+ + LA G L+ +DN ++ T GTV+LKA+F Sbjct: 228 TLPEEAVAGI-NRAQRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARF 286 Query: 321 PNTDYALFPSQFVNIRLQLNTEQGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGP 380 N D+ L+P Q+VN RLQ+ + A +P AAIQR +G +VY + + ++PVK Sbjct: 287 DNPDHKLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVER 346 Query: 381 VQDDLTVITDG--VSAGETVVIDGIDRLREGAKV 412 +QD + V+ G V AG+ VVIDG +L+ G+++ Sbjct: 347 IQDGIAVLAAGNAVQAGQRVVIDGQYKLKPGSRI 380 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 155 bits (391), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 110/322 (34%), Positives = 170/322 (52%), Gaps = 9/322 (2%) Query: 111 TATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARL 170 A VRARV G + K +KEG VK G +L +IDP +A+L A+ LAR +A L L Sbjct: 64 VAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTL 123 Query: 171 DLKRYQTLLAQDSIASQQVDTQAALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRL 230 +RY+ L+ ++++ Q+ D A KQ E V A + V +A L L + VT+PISGR+ Sbjct: 124 TAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRI 183 Query: 231 GLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPEDNIPKVLQQLQSGRKLPAQAWDR- 289 G G V D L ++ QL P+ + ++ K LQQ + +L + ++ Sbjct: 184 GRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLK-LQQAMAAGQLKSVGDNKA 242 Query: 290 ----EQKNKLA---DGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTE 342 E +N A G LL D VD TG+V L+A+FPN L P +V +++ + Sbjct: 243 SVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVD 302 Query: 343 QGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDG 402 + A ++P A+ R + G V VV ++ V RPVKTG + + + DG+ G+ V+++G Sbjct: 303 EQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEG 362 Query: 403 IDRLREGAKVEAVARGGADDPA 424 + ++R GA V+ V A PA Sbjct: 363 LQKVRPGATVKPVQWNPAGAPA 384 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 134 bits (336), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 105/337 (31%), Positives = 159/337 (47%), Gaps = 7/337 (2%) Query: 86 VGVAKARLQDVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPR 145 +GV Q V + G +P A VR +V+G + ++EG VKAG +L +I+P Sbjct: 41 LGVVTVHAQPVELHTELPGRTSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPA 100 Query: 146 SLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIASQQVDTQAALVKQYEGTVKA 205 S QAA + LA+ A + +ARL RY+ L+A +++ Q D A + + E V + Sbjct: 101 SYQAAYDSSVAALAKAEASVRTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGS 160 Query: 206 DEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIP 265 NV ++R+ L + R+ APISGR+G G VT + T L I QL PI + Sbjct: 161 ARANVQTSRINLDYARLDAPISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQS 220 Query: 266 ED---NIPKVL---QQLQSGRKLPAQAWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQ 319 N+ + L Q +SG + + +G L D V+ TG + L+A Sbjct: 221 STTWLNLKEALANGQLEKSGNGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAI 280 Query: 320 FPNTDYALFPSQFVNIRLQLNTEQGATVIPTAAIQRGSKGLFV-YVVKDDSSVTVRPVKT 378 FPN L P +V LQ Q ++P A+ R G V +VV V R + T Sbjct: 281 FPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVT 340 Query: 379 GPVQDDLTVITDGVSAGETVVIDGIDRLREGAKVEAV 415 D +++ G+ G+ +V+DG R GAKV+ V Sbjct: 341 DRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKVKVV 377 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 94/298 (31%), Positives = 148/298 (49%), Gaps = 2/298 (0%) Query: 114 VRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLK 173 VR V G L +H+K+G +VK GD+L IDPR +A + +AE QLA A DL Sbjct: 79 VRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEARAGYTASDLA 138 Query: 174 RYQTLLAQDSIASQQVDTQAALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLR 233 R Q LL+ ++IA + + + ++ V A + +ARL L +TR+TAP+SGR+ Sbjct: 139 RGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTRITAPVSGRISRA 198 Query: 234 QADVGNNVTTSDTNG-LVIITQLQPITAIFSIPEDNIPKVLQQLQ-SGRKLPAQAWDREQ 291 + VGN V N L + + + A F + E + K + + +G +P + + Sbjct: 199 EVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVNPARAAGATVPVELGLANE 258 Query: 292 KNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTA 351 G + ++DN +D +GT++++A F N + L P + +RL A +I Sbjct: 259 DGYSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYARVRLGGGEPHQAVLIDEK 318 Query: 352 AIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREG 409 AI FV V D+ R V G D L VI G+ GE +V++G+ R+R G Sbjct: 319 AIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPG 376 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.131 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,198,666 Number of extensions: 42044 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 11 Length of query: 516 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 427 Effective length of database: 1,198,455 Effective search space: 511740285 Effective search space used: 511740285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)