BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0029.0050.p
         (296 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310842|ref|YP_003774934.1| cobalamin biosynthesis transmem...   559   e-160
gi|300311955|ref|YP_003776047.1| cobalamin biosynthesis CobD/Cbi...    87   2e-18

>gi|300310842|ref|YP_003774934.1| cobalamin biosynthesis
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 319

 Score =  559 bits (1440), Expect = e-160,   Method: Compositional matrix adjust.
 Identities = 296/319 (92%), Positives = 296/319 (92%), Gaps = 23/319 (7%)

Query: 1   MTFLSILFALLIEQLKPLRADNPVYASIKSFAATIEGWFNAGQVHHGRLGWVAMVGALTV 60
           MTFLSILFALLIEQLKPLRADNPVYASIKSFAATIEGWFNAGQVHHGRLGWVAMVGALTV
Sbjct: 1   MTFLSILFALLIEQLKPLRADNPVYASIKSFAATIEGWFNAGQVHHGRLGWVAMVGALTV 60

Query: 61  PVALVYWLLLHVSLVAAFAWNVLVVYLTLGFRHYSHYFTSIQLALNSGDEMTARALLAEW 120
           PVALVYWLLLHVSLVAAFAWNVLVVYLTLGFRHYSHYFTSIQLALNSGDEMTARALLAEW
Sbjct: 61  PVALVYWLLLHVSLVAAFAWNVLVVYLTLGFRHYSHYFTSIQLALNSGDEMTARALLAEW 120

Query: 121 TRQDTTGMDVSEISRIASERALITTHRHVFGVFFWFLMPVGPALAVMYRVAEYLSRAWNE 180
           TRQDTTGMDVSEISRIASERALITTHRHVFGVFFWFLMPVGPALAVMYRVAEYLSRAWNE
Sbjct: 121 TRQDTTGMDVSEISRIASERALITTHRHVFGVFFWFLMPVGPALAVMYRVAEYLSRAWNE 180

Query: 181 PDHMKFEAFGRFAAQAFYWIDWIPARLTAAAFAVVGNFEDAIYAWRNFASRWDDESAGII 240
           PDHMKFEAFGRFAAQAFYWIDWIPARLTAAAFAVVGNFEDAIYAWRNFASRWDDESAGII
Sbjct: 181 PDHMKFEAFGRFAAQAFYWIDWIPARLTAAAFAVVGNFEDAIYAWRNFASRWDDESAGII 240

Query: 241 LA-----------------------ADAATVDSMPEETDGLPGDTPSPRTLQSTVGLVWR 277
           LA                       ADAATVDSMPEETDGLPGDTPSPRTLQSTVGLVWR
Sbjct: 241 LAAGGGAMGVRFGTEKEAAAGIILPADAATVDSMPEETDGLPGDTPSPRTLQSTVGLVWR 300

Query: 278 ALLLWMFLLLLLSVAVWMG 296
           ALLLWMFLLLLLSVAVWMG
Sbjct: 301 ALLLWMFLLLLLSVAVWMG 319


>gi|300311955|ref|YP_003776047.1| cobalamin biosynthesis CobD/CbiB
           [Herbaspirillum seropedicae SmR1]
          Length = 317

 Score = 87.0 bits (214), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 57/185 (30%), Positives = 90/185 (48%), Gaps = 2/185 (1%)

Query: 57  ALTVPVALVYWLLLHVSLVAAFAWNVLVVYLTLGFRHYSHYFTSIQLALNSGDEMTARAL 116
           A+  PV L  WL+       A   +  ++Y  +G R    +   I  AL +G+   AR L
Sbjct: 64  AVLPPVLLAAWLIAQTPWWLAALLHAGLLYFCIGLRSLREHTLPIAQALGAGELAQARRL 123

Query: 117 LAEWTRQDTTGMDVSEISRIASERALITTHRHVFGVFFWFLMPVGPALAVMYRVAEYLSR 176
            A    +DT      E+++   E  L   +  VFG  FWFL+  GP  A+++R++  L  
Sbjct: 124 TAWIVSRDTRSASEQELAKAGVESLLENGNDAVFGTLFWFLLAGGPG-ALLFRLSNTLDA 182

Query: 177 AWNEPDHMKFEAFGRFAAQAFYWIDWIPARLTAAAFAVVGNFEDAIYAWRNFASRWDDES 236
            W    + +F  FG  AA+    ++W PARLTA ++AV+G    A+  W+  A  W   +
Sbjct: 183 MWGY-RNARFLYFGWAAARIDDALNWCPARLTALSYAVLGQRRQALACWKEQAPAWSSPN 241

Query: 237 AGIIL 241
           AG ++
Sbjct: 242 AGPVM 246


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.137    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 958,401
Number of extensions: 38996
Number of successful extensions: 178
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 176
Number of HSP's successfully gapped: 2
Length of query: 296
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 211
Effective length of database: 1,217,395
Effective search space: 256870345
Effective search space used: 256870345
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 130 (54.7 bits)