BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0043.p (594 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 1216 0.0 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 477 e-135 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 399 e-112 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 129 5e-31 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 106 4e-24 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 1216 bits (3147), Expect = 0.0, Method: Compositional matrix adjust. Identities = 593/594 (99%), Positives = 593/594 (99%) Query: 1 MSYTAPVKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSAD 60 MSYTAPVKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSAD Sbjct: 1 MSYTAPVKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSAD 60 Query: 61 KEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANL 120 KEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANL Sbjct: 61 KEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANL 120 Query: 121 SFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAV 180 SFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAV Sbjct: 121 SFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAV 180 Query: 181 PEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGS 240 PEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGS Sbjct: 181 PEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGS 240 Query: 241 LGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAV 300 LGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAV Sbjct: 241 LGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAV 300 Query: 301 GIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARAL 360 GIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARAL Sbjct: 301 GIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARAL 360 Query: 361 SYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIE 420 SYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIE Sbjct: 361 SYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIE 420 Query: 421 ETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSELQGDAQ 480 ETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSELQGDAQ Sbjct: 421 ETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSELQGDAQ 480 Query: 481 LQPIAGRLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAAVS 540 LQPIA RLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAAVS Sbjct: 481 LQPIAARLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAAVS 540 Query: 541 AAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 AAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF Sbjct: 541 AAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 477 bits (1228), Expect = e-135, Method: Compositional matrix adjust. Identities = 260/600 (43%), Positives = 362/600 (60%), Gaps = 15/600 (2%) Query: 3 YTAPVKDMLFVMNELAGLAAV-NALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADK 61 Y AP++DM FV++EL + V LP D E + +LEE KF S+V+ PLNHS D+ Sbjct: 4 YNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGDR 63 Query: 62 EPSSW--ADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 E + VTT GFK+A++Q+ EAGW + EFGGQGLP + EMLNS+N Sbjct: 64 EGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSSN 123 Query: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 ++ + P L+ G E L G+++QK+ ++ +SG+WTGTM LTE G+DL L+R++A Sbjct: 124 QAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKA 183 Query: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 P+ DG+YK+ G KIFI+ GE+D++ NI+HLVLAR P+APEG KGISLF+VPKFL NADG Sbjct: 184 EPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNADG 243 Query: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 SLGARN + C +IE K+GI + T + GA G L+G+ ++GL+ MF+ MNAAR Sbjct: 244 SLGARNPIFCGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMNAARLG 300 Query: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAG-----SPGPVAIIHQPDVRRMLMSMRAQI 354 VG+QGL ++E AYQ A+ YA+DR+Q R L+G P I+H PDVRRML++ +A Sbjct: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVH-PDVRRMLLTAKAFA 359 Query: 355 EAARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHG 414 E RA + A D DE RK + L P+VK ++T+ + TS +QV+G Sbjct: 360 EGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYG 419 Query: 415 GMGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSE 474 G G+I E G QY RDARI IYEGT IQ+ DL+GRK D GA K AQ++ V E Sbjct: 420 GHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEE 479 Query: 475 LQGDAQLQPIAGRLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMA 534 D + L + D + ++ + ++ A +VPYL++ G ++ + A Sbjct: 480 HGTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFA 539 Query: 535 RAAAVSAAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 R A ++ K +G+ FY AKLATARFY ++LP+ G A +MAL E F Sbjct: 540 RMAKIALEKQDSGD---SFYAAKLATARFYFARLLPETAMLIRQARSGAANLMALDAELF 596 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 399 bits (1025), Expect = e-112, Method: Compositional matrix adjust. Identities = 229/537 (42%), Positives = 301/537 (56%), Gaps = 13/537 (2%) Query: 1 MSYTAPVKDMLFVMNE-LAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSA 59 M Y P++D FV++E L + ALP + + +LEE +F ++VVAPLN Sbjct: 3 MHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIG 62 Query: 60 DKEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 D+E + +G VTT PGF+ A+ QF GW + E GGQGLP +L EML++AN Sbjct: 63 DREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAAN 122 Query: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 + + P L G L G+ + ++ SG+W TM LTE QAGSDL +RTRA Sbjct: 123 HGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTRA 182 Query: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 VP+ DG+Y+L G KIFI+ GEHD+ NIVHLVLAR P+AP G KG+SLF+ P+ L + Sbjct: 183 VPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD--- 239 Query: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 G+RN VHC IEHK+GI S T L F A LVGE RGL MF+MMNAAR Sbjct: 240 --GSRNGVHCTGIEHKMGIHGSATCSLHFEQ---AQAWLVGEAGRGLAAMFVMMNAARLH 294 Query: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARA 359 VG QGL ++E AYQ+A AYAR+R QSR GS G I+H P V+++LM R +IE AR Sbjct: 295 VGAQGLGLAEAAYQRASAYARERRQSR-APGSSGNDLIVHHPAVQKLLMEQRCRIEGARL 353 Query: 360 LSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFI 419 L+ A D A + A R + E++ P++K ++T+ S +QV GG G++ Sbjct: 354 LTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYV 413 Query: 420 EETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSELQGDA 479 ETG Q+ RDARI IYEGT IQA D + RK D G L VR ++E G Sbjct: 414 VETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASG-- 471 Query: 480 QLQPIAGRLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMARA 536 L A LGEA D L + A + + L++ G V GW RA Sbjct: 472 -LGEQARLLGEAVDKLRIFASRIAAAAAQAPALPYYVADEMLRLCGHVALGWMWLRA 527 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 129 bits (325), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 114/382 (29%), Positives = 173/382 (45%), Gaps = 45/382 (11%) Query: 79 EAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEML----NSANLSFALCPLLTDGTIE 134 EA ++ E G G+ P ++GG G+ + +E + + + ++ L G E Sbjct: 38 EALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITE 97 Query: 135 ALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTYKLFGTKI 194 G+ QKE ++ G+ G LTEP GSD A +RTRA +GD + L GTK Sbjct: 98 KY---GSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERDGD-DFILNGTKQ 153 Query: 195 FITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCVSIEH 254 FIT G+H V +V A T + G KGIS F+VP + G + R+ E Sbjct: 154 FITSGKHAG----VAIVFAVTDRSA-GKKGISCFLVP---CDTPGFVVGRS-------EE 198 Query: 255 KLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQK 314 K+G AS T + + L+G+E G + A R + Q + ++ A++ Sbjct: 199 KMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEA 258 Query: 315 AVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVAAATSDAAHFS 374 AV+YAR R+ G P II V L M ++AAR + + AA D Sbjct: 259 AVSYAR----QRESFGVP----IIEHQAVNFRLADMNTLLDAARLMVWRAAQLKDQGRPC 310 Query: 375 DDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIEETGAAQYYRDARIL 434 EA+ + K + +E + + S +Q+HGG+G+ + + YRD RI Sbjct: 311 LKEAS-------------MAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRIC 357 Query: 435 PIYEGTTAIQANDLVGRKTARD 456 IYEG IQ ++GR A D Sbjct: 358 QIYEGANDIQ-RLVIGRAIASD 378 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 106 bits (265), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 109/361 (30%), Positives = 164/361 (45%), Gaps = 41/361 (11%) Query: 85 GEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLLTDGTIEALMTAGTQQQ 144 GE G+ G+ P GG GL +L AT E L A S A + + + + G Sbjct: 46 GELGFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSL 105 Query: 145 KETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTYKLFGTKIFIT-YGEHDM 203 K + +G+ G+ LTEP +GSD A ++T A E DG Y L G+K FI+ G D Sbjct: 106 KAQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTARLE-DGHYVLNGSKAFISGAGSTD- 163 Query: 204 AENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG-SLGARNDVHCVSIEHKLGIKASP 262 V +V+ART EG +G+S +VP NA G S G + E K+G + P Sbjct: 164 ----VLVVMART--GGEGARGVSAIVVPG---NAPGVSYGKK--------ESKMGWNSQP 206 Query: 263 TAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQKAVAYARDR 322 T + F + L+G+E +G + ++ R + + ++ A A AY ++R Sbjct: 207 TRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKER 266 Query: 323 IQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVAAATSDAAHFSDDEATRKS 382 Q G P I ++ L M+ ++ AAR + +AA DA EAT Sbjct: 267 KQF----GRP----IADFQALQFKLADMQTELVAARQMVRLAATKLDAG---SPEATT-- 313 Query: 383 NQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTA 442 + K T++ V + +Q+HGG G+I E +Y+RD R+ I EGT Sbjct: 314 -------YCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEGTNE 366 Query: 443 I 443 I Sbjct: 367 I 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,891,008 Number of extensions: 82282 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 9 Length of query: 594 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 503 Effective length of database: 1,188,985 Effective search space: 598059455 Effective search space used: 598059455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)