BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0039.p (313 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 625 e-180 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 97 1e-21 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 94 2e-20 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 63 3e-11 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 625 bits (1611), Expect = e-180, Method: Compositional matrix adjust. Identities = 312/313 (99%), Positives = 312/313 (99%) Query: 1 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG Sbjct: 1 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60 Query: 61 NDAFGRFIRQRVAQEGVEVSRVITDREFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSV 120 NDAFGRFIRQRVAQEGVEVSRVITD EFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSV Sbjct: 61 NDAFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSV 120 Query: 121 DDFDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQE 180 DDFDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQE Sbjct: 121 DDFDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQE 180 Query: 181 VMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRN 240 VMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRN Sbjct: 181 VMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRN 240 Query: 241 GQGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDM 300 GQGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDM Sbjct: 241 GQGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDM 300 Query: 301 EGLPTRAELEAAS 313 EGLPTRAELEAAS Sbjct: 301 EGLPTRAELEAAS 313 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 97/309 (31%), Positives = 131/309 (42%), Gaps = 8/309 (2%) Query: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 M ++ +GEA+ P + ++ Y G +N AI AR G + S VG+D Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGY----GGDTSNFAIAAARQGAASAYLSAVGDD 56 Query: 63 AFGRFIRQRVAQEGVEVSRVITDREFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 FG+ + +E V+V V T I G Y R GSAAS Sbjct: 57 HFGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHH--FHYRRAGSAASAYQAKA 114 Query: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQEVM 182 AA LH +GI+ A+S + AM R G T S D NLR LWP + Sbjct: 115 LPLEAIAAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERAR 174 Query: 183 AQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQ 242 A+ A D LP + +LTG +D I L GVKLV KLG EG Y Sbjct: 175 ARMEEAFRL-CDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSD 233 Query: 243 GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEG 302 VA PV + +D GAGD F ++ ++ G A N A + G +E Sbjct: 234 QRRMVAPYPV-DSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEP 292 Query: 303 LPTRAELEA 311 +P RA++EA Sbjct: 293 IPRRAQVEA 301 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 87/328 (26%), Positives = 142/328 (43%), Gaps = 26/328 (7%) Query: 4 QLDVVTWGEALALLVADEVG-PFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 +LD++ G L A +VG P +V + + L G+ N+A G ARLGLK +R+G++ Sbjct: 20 RLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDE 79 Query: 63 AFGRFIRQRVAQEGVEVSRVITDREFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 A G+F++ +A EG +VS V D + TA+ + + P I +YR A L+ +D Sbjct: 80 ANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSFPLI-FYRDNCADMALAPED 138 Query: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLW------ 176 D + +++ L TG + + + A+ + R D + RP+LW Sbjct: 139 IDEAFIASSKALLITGTHFSTPQVHRS-SSLALQYARRHNVRTVLDIDYRPVLWGLTSKG 197 Query: 177 ------PSQEVMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGV--KLVV 228 + + + Q L + + D V+ E I G D +A R + +V Sbjct: 198 DGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDI--MAALRAVRALCAATLV 255 Query: 229 IKLGAEGAYWRNGQGEGRV------AGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQA 282 +K G G +G R+ GV V EV++ +GAGD F G + G L Sbjct: 256 VKRGPLGCVVLHGAIPERLDDGFHYRGVQV-EVLNVLGAGDAFISGFLKGWLNEEDDEHC 314 Query: 283 VMRGNRIGAFAIQVVGDMEGLPTRAELE 310 N GA + +P EL+ Sbjct: 315 CRYANACGALVVSRHACAPAMPGPIELD 342 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 83/283 (29%), Positives = 117/283 (41%), Gaps = 34/283 (12%) Query: 37 GAETNVAIGLARLGLKVGWASRVGNDAFGRFIRQRVAQEGVEVS-RVITDREFRTAIQLK 95 G +N A+ AR+G V + S +G DAFG F +E + RVI A Sbjct: 45 GKGSNQAVAAARVGAPVRFISAIGKDAFGDFALSLWRRETIAPHVRVIDGAPTGAAFIYV 104 Query: 96 AKAVGGADPAIEYYRKGSAASHLSVDDFDAGYFGAARHLHATGIAPALSATTMAFAHQA- 154 A G D AI AAS LS D + R A A T + +A Sbjct: 105 NDASG--DNAIIVVP--GAASQLSEQDVE-------RERQAIEAAKVF-VTQLEQPPEAA 152 Query: 155 ---MDFMRGQGKTISFDPNLRPML-WPSQEVMAQQLNALAFKADWVLPGLSEGKILTG-- 208 + R G T F+P P L +P+Q + D++ P E +LTG Sbjct: 153 LAGLQIARAAGTTTVFNP--APALAFPAQ---------VYGLCDFITPNEHEAALLTGIE 201 Query: 209 --H-DDAREIAGFYLERGVKLVVIKLGAEGAYWRNGQGEGRVAGVPVKEVVDTVGAGDGF 265 H DDAR A LERGV +I LGA+G+ + + + VV+T GAGD F Sbjct: 202 IHHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLPALCAGTVVETAGAGDAF 261 Query: 266 AVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDMEGLPTRAE 308 +G+ EG+ +A + A ++ G +P+R E Sbjct: 262 NGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMPSRQE 304 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,083,020 Number of extensions: 51112 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 6 Length of query: 313 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 228 Effective length of database: 1,217,395 Effective search space: 277566060 Effective search space used: 277566060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)