BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0021.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 604 e-174 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 115 3e-27 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 91 2e-19 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 80 2e-16 gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 72 7e-14 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 604 bits (1557), Expect = e-174, Method: Compositional matrix adjust. Identities = 306/309 (99%), Positives = 307/309 (99%) Query: 1 MRAICYHQYGPPSVLHLCDLDEHPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 MRAICYHQYGPPSVLHLCDLDE PLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 Query: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ Sbjct: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 Query: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSN 180 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSN Sbjct: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSN 180 Query: 181 VEYVAALGASRVLAYDRDELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQLVWLVAAPIR 240 VEYVAALGASRVLAYDRDELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQLVWLVAAPIR Sbjct: 181 VEYVAALGASRVLAYDRDELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQLVWLVAAPIR 240 Query: 241 ERGEEFGVCVRRALIVEDQSILQQVAALAQQGVWRPAVSGVLPMQEIARAHQRIEAGEIT 300 ERGEEFGV VRRALIVEDQSILQQVAALAQQGVWRPAVSGVLPMQEIARAHQRIEAGE+T Sbjct: 241 ERGEEFGVSVRRALIVEDQSILQQVAALAQQGVWRPAVSGVLPMQEIARAHQRIEAGEVT 300 Query: 301 RGRLVLDIG 309 RGRLVLDIG Sbjct: 301 RGRLVLDIG 309 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 115 bits (289), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 107/336 (31%), Positives = 153/336 (45%), Gaps = 31/336 (9%) Query: 1 MRAICYHQYGPPSVLHLCDLDEHPLPPGHVRVAMQAAGVAPVDTKLRAG------RLQHL 54 M+AI H +G P VL D L PG V V + A G+ P D LR G Q Sbjct: 6 MKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPT 65 Query: 55 VALDLPKVPGRDGAGVITALGEGVHTLQVGEAVCVM---PS-MAGRGTTLAELV-LPQEQ 109 VA P + G D +GV+ A+ + V VG+ V M PS +AG A+ V + + Sbjct: 66 VA--FPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASE 123 Query: 110 VVRKPDVLSMQQAAALLQPACSAWIALMQTA-------------PIAAGMK-VFIHAGSG 155 + KP +S QAAA +AW +++ P+ K V ++ +G Sbjct: 124 IALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAG 183 Query: 156 AVGSLMVQLAAHLGAEVTASCRSSNVEYVAALGASRVLAYDRDELGRV-RDQDVVIDLVG 214 VG +QLA GA V A + ++ LGA + Y R V D D+VID VG Sbjct: 184 GVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVIDAVG 243 Query: 215 GAAHDQSYRMLRRGGQL--VWLVAAPIRERGEEFGVCVRRALIVEDQSILQQVAALAQQG 272 G A + R L+RGG L ++ + E GV V + + LQQ+A L G Sbjct: 244 GPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLLDSG 303 Query: 273 VWRPAVSGVLPMQEIARAHQRIEAGEITRGRLVLDI 308 V R A+ +Q+ +AH+R G I RG++VL + Sbjct: 304 VVRVAIDSTFALQDAGKAHERAAQGHI-RGKIVLSV 338 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 87/331 (26%), Positives = 145/331 (43%), Gaps = 29/331 (8%) Query: 1 MRAICYHQYGPPSVLHLCDLDEHPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 MRAI ++G P VL C+ L G V + + AAGV D R G + V Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTG--NYPVPPGAS 58 Query: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 +PG + AG I A G ++G+ VC + G A P Q + P LS Sbjct: 59 DLPGLEVAGEIVAGDLGDSGFKLGDLVCALVQGGGYAEFCAA---PLAQCLPVPKGLSAL 115 Query: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSS- 179 +AAAL + + W + ++ + + GS +G +Q+A LG V A+ S+ Sbjct: 116 EAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAE 175 Query: 180 NVEYVAALGASRVLAYDRDELGRV-------RDQDVVIDLVGGAAHDQSYRMLRRGGQLV 232 LGA R + Y ++ V R DV++D+VGG + + L G+LV Sbjct: 176 KCRACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLV 235 Query: 233 WLVAAPIRERGEEFGVCVRRALIVEDQSILQQ-------VAALAQQGVW--------RPA 277 ++ + + G +RR L + ++ + +AA ++ VW RP Sbjct: 236 FIAQLGGAKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPV 295 Query: 278 VSGVLPMQEIARAHQRIEAGEITRGRLVLDI 308 + P+++ A+AH +E G G+++L++ Sbjct: 296 IHHRFPLEQAAQAHAMME-GSTHIGKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 75/244 (30%), Positives = 111/244 (45%), Gaps = 20/244 (8%) Query: 2 RAICYHQYGPPSVLHLCDLDEHPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLPK 61 +AI + G P V+ D++ G VR+ A G+ +D RAG L LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAG----LYPQPLPA 58 Query: 62 VPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGR--GTTLAELVLPQEQVVRKPDVLSM 119 G++ AG I A+G GV +VG+ V + AGR G E V+P + +V+ PD +S Sbjct: 59 GLGQEAAGTIEAVGAGVSGFKVGDRV----AYAGRPNGAYAEERVMPADILVKLPDAISF 114 Query: 120 QQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSS 179 AAA++ + L QT + G + HA +G +G + Q A LG + + SS Sbjct: 115 DTAAAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTV-SS 173 Query: 180 NVEYVAAL--GASRVLAYDRD-------ELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQ 230 + + AL GA+ V+ Y + EL R VV D +G S LR G Sbjct: 174 DEKAALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGM 233 Query: 231 LVWL 234 +V Sbjct: 234 MVSF 237 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 71.6 bits (174), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 96/342 (28%), Positives = 153/342 (44%), Gaps = 46/342 (13%) Query: 1 MRAICYHQYGP----PSVLHLCDLDEHPLPPGH-VRVAMQAAGVAPVDTKLRAGRLQHLV 55 M+AI P P L L + P P H + V ++A V PVDTK+RA + Sbjct: 1 MKAILTQPQSPLISAPDALIETTLPD-PTPGEHDLLVEVKAISVNPVDTKIRASAKE--- 56 Query: 56 ALDLPKVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVV-RKP 114 +V G D +GV+ A+G V G+ V S+ G +EL + E++V RKP Sbjct: 57 ----ARVLGWDVSGVVRAVGPAVTLFAPGDEVFYAGSITRPGAN-SELHVVDERIVGRKP 111 Query: 115 DVLSMQQAAALLQPACSAWIALMQTAPIAA----GMKVFIHAGSGAVGSLMVQLAAHLG- 169 L AAA+ A +AW L +A G + I +G VGS++ QLA L Sbjct: 112 SSLGHADAAAIPLTAITAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQ 171 Query: 170 -AEVTASCRSSNVEYVAALGASRVLAYDR------DELGRVRDQDVVIDLVGGAAH-DQS 221 + + R +V LGA V+ + + ++LG + + +VI L H Q Sbjct: 172 LTVIGTASRPETAHWVGELGAHHVIDHSQPLKPQLEKLG-IPEVGIVISLTHTDQHYAQI 230 Query: 222 YRMLRRGGQLVW------LVAAPIRER--GEEFGVCVRRAL-----IVEDQSILQQVAAL 268 +L GQL L A P++ + + + R++ ++ +LQ+V+ L Sbjct: 231 IDVLAPQGQLALIDDPASLDAMPLKRKSISLHWELMFTRSMFQTPDMIRQHELLQRVSQL 290 Query: 269 AQQGVWRPAVS---GVLPMQEIARAHQRIEAGEITRGRLVLD 307 QGV R G + + RAH +E+G+ +G++VL+ Sbjct: 291 LDQGVLRTTTGEHYGRIDAANLRRAHGLLESGK-AKGKIVLE 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,040,636 Number of extensions: 45517 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 5 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)