BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0008.p (283 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 561 e-161 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 192 3e-50 gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 108 4e-25 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 89 6e-19 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 87 1e-18 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 561 bits (1445), Expect = e-161, Method: Compositional matrix adjust. Identities = 283/283 (100%), Positives = 283/283 (100%) Query: 1 MWKLFKPNHKNIRFWQLMVLAIVLVVWHVATRNPQTAFFFGEPLKVAQRIWEWFTVGSGS 60 MWKLFKPNHKNIRFWQLMVLAIVLVVWHVATRNPQTAFFFGEPLKVAQRIWEWFTVGSGS Sbjct: 1 MWKLFKPNHKNIRFWQLMVLAIVLVVWHVATRNPQTAFFFGEPLKVAQRIWEWFTVGSGS 60 Query: 61 LEVGIGDTTFFTLSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDP 120 LEVGIGDTTFFTLSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDP Sbjct: 61 LEVGIGDTTFFTLSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDP 120 Query: 121 YVKAANSMPRVILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANAR 180 YVKAANSMPRVILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANAR Sbjct: 121 YVKAANSMPRVILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANAR 180 Query: 181 MLGANARQLLRTVYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTF 240 MLGANARQLLRTVYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTF Sbjct: 181 MLGANARQLLRTVYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTF 240 Query: 241 DINTVFAGIVVLTIFALVLDLAVGVIEKRLMKWQPKSGETEKL 283 DINTVFAGIVVLTIFALVLDLAVGVIEKRLMKWQPKSGETEKL Sbjct: 241 DINTVFAGIVVLTIFALVLDLAVGVIEKRLMKWQPKSGETEKL 283 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 192 bits (487), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 112/266 (42%), Positives = 168/266 (63%), Gaps = 23/266 (8%) Query: 16 QLMVLAIVLVVWHVATR----NPQTAFFFGEPLKVAQRIWEWFTVGSGSLEVGIGDTTFF 71 ++ +L IVL W V R +P FFF +P + +I++W G+ Sbjct: 37 RIAILVIVLGGWEVCARIGWIDP---FFFSQPSLIVAQIYDWMVEGTSQ----------- 82 Query: 72 TLSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRV 131 +++ +L+TL ETVL F+IG+V G+ G+ L + S + Y++ ANS+PRV Sbjct: 83 -----GPLWTQVLVTLEETVLGFLIGSVAGVIAGIVLGRNKLLSDVFSLYIQIANSIPRV 137 Query: 132 ILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLR 191 +L IF + FGLG+ SKVALAV +VFF+VF N +QGV+E ++ANA++LGA+ RQ+ Sbjct: 138 VLGSIFVIAFGLGMASKVALAVVMVFFVVFANAFQGVREADRYMIANAQILGASRRQVTM 197 Query: 192 TVYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVV 251 V +PSA SW+ +SLH S G A VGAVVGE+LGS +G+G LI A+G F+ + VFA ++V Sbjct: 198 AVVIPSALSWILASLHVSFGFALVGAVVGEFLGSKQGIGLLISTAQGAFNASGVFAAMIV 257 Query: 252 LTIFALVLDLAVGVIEKRLMKWQPKS 277 L + AL D + +E+RL+KW+P++ Sbjct: 258 LAVVALAADWLLHALERRLLKWRPQA 283 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 108 bits (270), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 64/201 (31%), Positives = 103/201 (51%) Query: 73 LSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVI 132 + + I H L T T++ F I V G+ +G + SP A P + A N++P+ Sbjct: 54 VDYAGPITEHALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAA 113 Query: 133 LAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRT 192 P+ +WFG+G + A + FF + N+ G+ + P + R+LGA +L Sbjct: 114 FVPVLVVWFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLK 173 Query: 193 VYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVL 252 V LP + + F+SL +I LAFVG+ V E S +G+GYL++ A + + FAG+VV+ Sbjct: 174 VGLPRSMPYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVI 233 Query: 253 TIFALVLDLAVGVIEKRLMKW 273 A+ + +IEKR W Sbjct: 234 GAMAMAMYELFAIIEKRTTGW 254 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 72/263 (27%), Positives = 126/263 (47%), Gaps = 12/263 (4%) Query: 17 LMVLAIVLVVWHVATR----NPQTAFFFGEPLKVAQRIWEWFT-VGSGSLEVGIGDTTFF 71 L+V IV+ +W A+R NPQ P V ++ E+ T + E G ++ Sbjct: 13 LLVPIIVIALWEGASRAGWINPQ---ILPSPWAVLRKWVEYATPLKPYDPEAG----SWL 65 Query: 72 TLSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRV 131 +F E+ + +L V F+IG L +GL + S +++ V+ +P + Sbjct: 66 AWAFSGELVMDAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPI 125 Query: 132 ILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLR 191 P+ +WFGLG + L FF V N GV++V + L AR LGA+ L Sbjct: 126 AYIPLAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFL 185 Query: 192 TVYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVV 251 V LP+A ++ S + IG AF+ +V E + + G+G+ I++A F + + AG++ Sbjct: 186 RVMLPAAVPYILSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFWSDKIIAGMIT 245 Query: 252 LTIFALVLDLAVGVIEKRLMKWQ 274 + + L +DL + + +++W Sbjct: 246 IGLLGLAIDLGMSRLNNYMLRWH 268 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 53/214 (24%), Positives = 111/214 (51%) Query: 69 TFFTLSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSM 128 T ++L+ E+ H+ +L FVIG+ +A+GL + +S A A+L+P +A ++ Sbjct: 62 TLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAI 121 Query: 129 PRVILAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQ 188 P + P+ +W G+ KV L FF V+ N+ G++++ ++ G N + Sbjct: 122 PSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQA 181 Query: 189 LLRTVYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAG 248 L+ + LP++ ++F+ L + LA++ V E + + +GVGYL+ + + V A Sbjct: 182 LIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELIAATKGVGYLLSDGRESSRPDLVLAA 241 Query: 249 IVVLTIFALVLDLAVGVIEKRLMKWQPKSGETEK 282 I++L + + D + +E++ + W+ ++ + Sbjct: 242 ILILALLGKLSDGLLRAVEQKSLGWREARRQSSQ 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 898,655 Number of extensions: 38308 Number of successful extensions: 261 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 257 Number of HSP's successfully gapped: 14 Length of query: 283 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 199 Effective length of database: 1,222,130 Effective search space: 243203870 Effective search space used: 243203870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)