BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0004.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 751 0.0 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 499 e-142 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 365 e-102 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 339 3e-94 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 289 4e-79 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 751 bits (1939), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/481 (74%), Positives = 408/481 (84%) Query: 1 MLTLNDPDLFRMQCPIGGHWQDAQDGATIAVTDPATGLTLGTVPAMGTAETRAAVQAAQA 60 ML L DP L R Q + G W DA G +AV +PA G LG VP MG AETR A++AA A Sbjct: 1 MLQLKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANA 60 Query: 61 ALPGWRARTAKERATLLRRWHALLMENQADLALLMTAEQGKPLAEASGEIAFAASFIEWF 120 A P W+ +TAKER+ +LRRW+ L++ N DLAL+MTAEQGKPLAEA GEI +AASFIEWF Sbjct: 61 AWPAWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWF 120 Query: 121 AEEARRINGDVIASPSSDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVI 180 AEE +R GD I SPS R++ +++ +GVCAAITPWNFPAAMITRKA PALAAGC MV+ Sbjct: 121 AEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVL 180 Query: 181 RPASQTPYSALAMVALAERAGIPPGVLNVVTGHPEAIGAELTSNPVVRKLSFTGSTAVGA 240 +PA TP+SALA+ LAERAGIP GV +VVTG P+ IG E+TSNP+VRK+SFTGST VG Sbjct: 181 KPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGK 240 Query: 241 RLMAQGAGHIQKLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCANRIYVQD 300 LM Q A I+KLSLELGGNAPFIVFDDADLDAA+EGA+ SKYRN GQTCVCANRIYVQD Sbjct: 241 LLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQD 300 Query: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK Sbjct: 301 GVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGK 360 Query: 361 RHALGHSFFEPTVLADVTPAMRVAREEIFGPVAPVLRFESEEEVIALANETEYGLASYFY 420 RHALGHSFFEPTVLADVTPAM+VAREE FGP+AP+ RF+++EEV+ALAN+TE+GLASYFY Sbjct: 361 RHALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFY 420 Query: 421 TRDMARVWRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELKYLC 480 +RD+ R+WRVAE LE GMVG+NTGLIS APFGGVKQSGLGREGS YGI++YL +KYLC Sbjct: 421 SRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 Query: 481 L 481 + Sbjct: 481 M 481 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 499 bits (1284), Expect = e-142, Method: Compositional matrix adjust. Identities = 277/487 (56%), Positives = 355/487 (72%), Gaps = 9/487 (1%) Query: 2 LTLNDPDLFRMQCPIGGHWQDAQDGATIAVTDPATGLTLGTVPAMGTAETRAAVQAAQAA 61 LTL DL IG W+ A DG + V+DPATG +VP G A+ RAAV+AA AA Sbjct: 4 LTLQRQDLMPGAQLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAA 63 Query: 62 LPGWRARTAKERATLLRRWHALLMENQADLALLMTAEQGKPLAEASGEIAFAASFIEWFA 121 WRA AK+RA +++RW+ LL+ +Q DL L++ EQGKPLAEA GE+A+AAS++EWF Sbjct: 64 FAAWRATPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFG 123 Query: 122 EEARRINGDVIASPSSDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIR 181 EEA R NGD+I +P + RR++ L++PVGV AAITPWNFPAAMI RK APALAAGCT+V + Sbjct: 124 EEATRANGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCK 183 Query: 182 PASQTPYSALAMVALAERAGIPPGVLNVVTGH----PEAIGAELTSNPVVRKLSFTGSTA 237 PA TP ++LA+V LA+ AG+P GV+N+VT PE + L ++ VRK+SFTGSTA Sbjct: 184 PAEDTPLTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWL-ADGRVRKISFTGSTA 242 Query: 238 VGARLMAQGAGHIQKLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCANRIY 297 VG L A ++KLSLELGGNAPFIVFDDAD+DAAI+G M +K+RNGGQTCV NRIY Sbjct: 243 VGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIY 302 Query: 298 VQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLL 357 VQ+ VYDAF KL V KVG T+ GP+IN +A+ K++QHV DA+A+GARV+ Sbjct: 303 VQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVIT 362 Query: 358 GGKR---HALG-HSFFEPTVLADVTPAMRVAREEIFGPVAPVLRFESEEEVIALANETEY 413 GGKR G +++ PTVLADVT AM+ + EE FGPVAP+ RF +E+EVIA AN T + Sbjct: 363 GGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPF 422 Query: 414 GLASYFYTRDMARVWRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEY 473 GLA+YFY+ D+ R+ RV +ALE G+VGVN G ++ APFGGVK+SG GREGS +G+++Y Sbjct: 423 GLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDY 482 Query: 474 LELKYLC 480 L KY+C Sbjct: 483 LHTKYVC 489 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 365 bits (938), Expect = e-102, Method: Compositional matrix adjust. Identities = 194/469 (41%), Positives = 279/469 (59%) Query: 12 MQCPIGGHWQDAQDGATIAVTDPATGLTLGTVPAMGTAETRAAVQAAQAALPGWRARTAK 71 +Q I G W + G TI V +PAT +G + A+ A++AA WR +A Sbjct: 5 VQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAF 64 Query: 72 ERATLLRRWHALLMENQADLALLMTAEQGKPLAEASGEIAFAASFIEWFAEEARRINGDV 131 ER+ ++RR LL E ++A LMT EQGKPLAEA E AA I+WFAEEARR G + Sbjct: 65 ERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRL 124 Query: 132 IASPSSDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIRPASQTPYSAL 191 + + + +++PVG AA TPWNFP + RK + ALAAGC+++++ +TP S Sbjct: 125 VPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPA 184 Query: 192 AMVALAERAGIPPGVLNVVTGHPEAIGAELTSNPVVRKLSFTGSTAVGARLMAQGAGHIQ 251 ++ AG+P GV+N+V G P I L +PV++K+SFTGST VG +L A H++ Sbjct: 185 ELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 Query: 252 KLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCANRIYVQDGVYDAFAAKLV 311 + ++ELGG+AP +VFDDAD+D A + +K+RN GQ CV R VQ GV++AF K V Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 312 EAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEP 371 ++ KVG G E G+T GP+ N++ + +E + DAV++GA++ GGKR FF+P Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQP 364 Query: 372 TVLADVTPAMRVAREEIFGPVAPVLRFESEEEVIALANETEYGLASYFYTRDMARVWRVA 431 TVL ++ R EE FGP+A + FE+ +EVI AN YGLA+Y + + +A Sbjct: 365 TVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALA 424 Query: 432 EALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELKYLC 480 +E GM+ +N ++ E PFGG+K SG G EG IE Y+ K + Sbjct: 425 ARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 339 bits (869), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 201/474 (42%), Positives = 282/474 (59%), Gaps = 3/474 (0%) Query: 7 PDLFRMQCPIGGHWQDAQDGATIAVTDPATGLTLGTVPAMGTAETRAAVQAAQAALPGWR 66 PDL Q I G + + V DPATG LG +P A+ A+QAA A WR Sbjct: 3 PDL---QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWR 59 Query: 67 ARTAKERATLLRRWHALLMENQADLALLMTAEQGKPLAEASGEIAFAASFIEWFAEEARR 126 + ER+ +LRR AL E +A +T + GKPLA+A EI AA ++W AEE RR Sbjct: 60 QVSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRR 119 Query: 127 INGDVIASPSSDRRLLTLRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIRPASQT 186 + G V+ + + R L +R+PVGVCAAI+PWNFP +K A A+AAGCT+V++ S++ Sbjct: 120 LYGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSES 179 Query: 187 PYSALAMVALAERAGIPPGVLNVVTGHPEAIGAELTSNPVVRKLSFTGSTAVGARLMAQG 246 P + + M L AG+P GV N+V+G I L +P+VR +SFTGS VG++L AQ Sbjct: 180 PAAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQA 239 Query: 247 AGHIQKLSLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCANRIYVQDGVYDAF 306 A H++++S+ELGG+AP +VFDDA+++ A K RN GQ CV +R YVQ G+Y F Sbjct: 240 ALHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARF 299 Query: 307 AAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGH 366 EA+ + KVG G E GV GPLI+ + + V+ + DA+A+G+ ++ GG R Sbjct: 300 VTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRG 359 Query: 367 SFFEPTVLADVTPAMRVAREEIFGPVAPVLRFESEEEVIALANETEYGLASYFYTRDMAR 426 F+ PT++ V P+ RV +EE FGP+A V F EE IA+AN GLASY +T+ + Sbjct: 360 HFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDT 419 Query: 427 VWRVAEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELKYLC 480 RV+ L+ GMV +N ++ E PFGG+ +SG G EG G E +L K + Sbjct: 420 AHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMIS 473 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 289 bits (739), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 177/467 (37%), Positives = 254/467 (54%), Gaps = 12/467 (2%) Query: 16 IGGHWQDAQDGATIAVTDPATGLTLGTVPAMGTAETRAAVQAAQAALPGWRARTAKERAT 75 I G WQ Q AV PAT + + A+ AVQAA+ A+P W A + ERA Sbjct: 20 IDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERAA 79 Query: 76 LLRRWHALLMENQADLALLMTAEQGKPLAEASGEIAFAASFIEWFAEEARRINGDVIASP 135 LL R HALL+E A +T E G P++ A + + AE R ++ P Sbjct: 80 LLGRVHALLLERSELFAQALTLEMGAPISYAR------TAHVPLAAEHLRVARDNLADYP 133 Query: 136 SSDRRLLT--LRQPVGVCAAITPWNFPAAMITRKAAPALAAGCTMVIRPASQTPYSALAM 193 R T +R+P+GVCA ITPWN+P IT K PALAAGCT+V++P+ +P SAL Sbjct: 134 FIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLF 193 Query: 194 VALAERAGIPPGVLNVVTGHPEAIGAELTSNPVVRKLSFTGSTAVGARLMAQGAGHIQKL 253 + AG+P GV N+V G +GA L+++P V +S TGST G + A ++++ Sbjct: 194 AEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRV 253 Query: 254 SLELGGNAPFIVFDDADLDAAIEGAMISKYRNGGQTCVCANRIYVQDGVYDAFAAKLVEA 313 + ELGG +P +V DADL+ AI + + RN GQ+C R+ V + Sbjct: 254 AQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALAT 313 Query: 314 VKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLG--GKRHALGHSFF-E 370 + + KVG + T GPL N +V+Q + + GAR+L G G+ L ++ Sbjct: 314 LAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYAR 373 Query: 371 PTVLADVTPAMRVAREEIFGPVAPVLRFESEEEVIALANETEYGLASYFYTRDMARVWRV 430 PT+ ++V M +A+EEIFGPV +L +++ EE IA+AN+T YGL ++ RD +V V Sbjct: 374 PTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAV 433 Query: 431 AEALEFGMVGVNTGLISTAEAPFGGVKQSGLGREGSKYGIEEYLELK 477 A ++ G V +N +APFGG KQSG GRE G+EEY+E+K Sbjct: 434 AARIQSGQVHLNYPAWDP-QAPFGGYKQSGNGREYGVEGMEEYMEVK 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,535,453 Number of extensions: 67206 Number of successful extensions: 339 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 291 Number of HSP's successfully gapped: 21 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)