BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0002.p (686 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313394|ref|YP_003777486.1| glucosidase [Herbaspirillum ser... 120 2e-28 >gi|300313394|ref|YP_003777486.1| glucosidase [Herbaspirillum seropedicae SmR1] Length = 803 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 102/390 (26%), Positives = 156/390 (40%), Gaps = 34/390 (8%) Query: 300 TSFGKRLFWDWRWNEQRYPGHAEWLAQLKQQGVRFLGYVNPYLCVDGTLYREAHAKGFLA 359 TS G + + + WN +++P ++ + + P L D + +A G L Sbjct: 316 TSIGPKRY-VFHWNTEKFPDPHGFVRSYLDASIHLCANIKPCLLRDHPQFEQARQAGLLI 374 Query: 360 TRADGSDYLVDFGEFDCGVVDFTQPEASRWFEQRVIGAEMLAQGLDG----------WMA 409 + G V F + +DFT P W+ V A +L G+ W Sbjct: 375 CDSQGEPAWVQFWDEVGAYLDFTNPATLDWWRANVKQA-LLDYGVSATWNDNNEFEVWTG 433 Query: 410 D-----FGEYLPTDVTLANGQPAMLMHNAWPTLWAEVNARAIADAGRTGDAVFFMRAGYT 464 D FG+ P V A MLM A + +A +A R+G Sbjct: 434 DAYAHGFGQQYP--VRQAKVLQTMLMMQA--------SRQAQLEAAPQRRPFLVSRSGGA 483 Query: 465 GVQAHCPLLWAGDQSVDFSRHDGLQTVICGALSSGFLGNAYHHSDIGGYTSLFGNRRTPE 524 G+Q + W+GD +++ + L+ + L G + DIGG++ G PE Sbjct: 484 GMQRYVQT-WSGD---NYTSWETLRYNLKMGLGLALSGVSNIGHDIGGFS---GPAPAPE 536 Query: 525 LFQRWAEMAAFTPMMRTHEGNRPDENFQFWQDPAVLAHFARMTRLFVGLKPYVQELVEQA 584 L RW F P H N + W P V A A + +L PY+ EL+ Q+ Sbjct: 537 LLVRWVAFGVFMPRFSIHSWNDDKTVNEPWMYPEVTAQIAALIKLRYRFIPYLYELLWQS 596 Query: 585 ATRGTPLQRPLFLHYEQDRATYTLQDQYLYGPDLLVAPVHAAQASRWSAYLPLGDDWLHL 644 P+ RP F + DR D + G +LVAPV + YLP G W+ Sbjct: 597 TQDYQPVLRPTFAEFPHDRRCLDECDDMMLGSAMLVAPVVEQGQTERRVYLPSGARWVSY 656 Query: 645 WSGQRHAGGQRVEVEAPLGQPPVFVRAASA 674 WSG+R+ GGQ + + AP QP + +R + Sbjct: 657 WSGERYEGGQEISLPAPWDQPVMLLREGAV 686 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,484,268 Number of extensions: 113856 Number of successful extensions: 277 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 276 Number of HSP's successfully gapped: 1 Length of query: 686 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 594 Effective length of database: 1,184,250 Effective search space: 703444500 Effective search space used: 703444500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)