BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0326.p (677 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 1364 0.0 gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 132 1e-31 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 90 7e-19 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 70 6e-13 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 69 1e-12 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 1364 bits (3530), Expect = 0.0, Method: Compositional matrix adjust. Identities = 676/677 (99%), Positives = 676/677 (99%) Query: 1 MQKNNDGLRPSSADIQNSASVEYFAASHAHYSSMATLAMGLAMAVILLGLVVLLASLLGE 60 MQKNNDGLRPSSADIQNSASVEYFAASHAHYSSMATLAMGLAMAVILLGLVVLLASLLGE Sbjct: 1 MQKNNDGLRPSSADIQNSASVEYFAASHAHYSSMATLAMGLAMAVILLGLVVLLASLLGE 60 Query: 61 EYRRLLSGLLEMREMTAVGLILGASALYLRCIHGGARRSGLRRVAAGLSWMLLALGLGTL 120 EYRRLLSGLLEMREMTAVGLILGASALYLRCIHGGARRSGLRRVAAGLSWMLLALGLGTL Sbjct: 61 EYRRLLSGLLEMREMTAVGLILGASALYLRCIHGGARRSGLRRVAAGLSWMLLALGLGTL 120 Query: 121 AHGLMYALGWLPIDQNHPLSHLVIPPLLSLGFVVMAAVLLLQDWRMLGGYYPAEYLSFAL 180 AHGLMYALGWLPIDQNHPLS LVIPPLLSLGFVVMAAVLLLQDWRMLGGYYPAEYLSFAL Sbjct: 121 AHGLMYALGWLPIDQNHPLSPLVIPPLLSLGFVVMAAVLLLQDWRMLGGYYPAEYLSFAL 180 Query: 181 IGLAAIPLVGYLYGVSQFVYLDFPLPVSLLSALVMALLATALLMARPAHPLMAIITRIAP 240 IGLAAIPLVGYLYGVSQFVYLDFPLPVSLLSALVMALLATALLMARPAHPLMAIITRIAP Sbjct: 181 IGLAAIPLVGYLYGVSQFVYLDFPLPVSLLSALVMALLATALLMARPAHPLMAIITRIAP 240 Query: 241 GGQMLRHLLPQTVFLLLAFSLLLNWGMTHGWILPELLLPTLTLINGVIVLFIFWGSASRL 300 GGQMLRHLLPQTVFLLLAFSLLLNWGMTHGWILPELLLPTLTLINGVIVLFIFWGSASRL Sbjct: 241 GGQMLRHLLPQTVFLLLAFSLLLNWGMTHGWILPELLLPTLTLINGVIVLFIFWGSASRL 300 Query: 301 DSEYGERTRNAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETM 360 DSEYGERTRNAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETM Sbjct: 301 DSEYGERTRNAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETM 360 Query: 361 YRSSRELFLVPEEADTIDRDDRRVMASGKPEKLEQTLHLPEGVVTFQTAKVPWFGKDGSV 420 YRSSRELFLVPEEADTIDRDDRRVMASGKPEKLEQTLHLPEGVVTFQTAKVPWFGKDGSV Sbjct: 361 YRSSRELFLVPEEADTIDRDDRRVMASGKPEKLEQTLHLPEGVVTFQTAKVPWFGKDGSV 420 Query: 421 QGVIGISTDITERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIAREL 480 QGVIGISTDITERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIAREL Sbjct: 421 QGVIGISTDITERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIAREL 480 Query: 481 HDNMGASLTALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDL 540 HDNMGASLTALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDL Sbjct: 481 HDNMGASLTALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDL 540 Query: 541 FGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASK 600 FGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASK Sbjct: 541 FGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASK 600 Query: 601 VDVILDIDEDHVALTVRDDGVGIPEERRDNHKTYGLRGLRERATFFGGEVDIRSTPGKGA 660 VDVILDIDEDHVALTVRDDGVGIPEERRDNHKTYGLRGLRERATFFGGEVDIRSTPGKGA Sbjct: 601 VDVILDIDEDHVALTVRDDGVGIPEERRDNHKTYGLRGLRERATFFGGEVDIRSTPGKGA 660 Query: 661 LITISLPIRPELVPSEA 677 LITISLPIRPELVPSEA Sbjct: 661 LITISLPIRPELVPSEA 677 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 132 bits (332), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 111/376 (29%), Positives = 183/376 (48%), Gaps = 41/376 (10%) Query: 310 NAQKLAETSALLNAVSESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETMYRSSRELFL 369 +AQ L E A NA + P ++K+ Q + NPA G T + + EL + Sbjct: 121 SAQALREMQARFNAFMNAAPMPTWIKDAQLRYSYVNPAQSAFYGMTPGDMDGLTDLEL-M 179 Query: 370 VPEEADTIDRDDRRVMASGKPEKLEQTLHLPEGVVT-------FQTAKVPWFGKDGSVQG 422 A +DR+V+ +K E LH E V + P G +G Sbjct: 180 SSGAAAASQENDRKVL----EQKCE--LHTQETVFDHNHNARILDVVRFPISG-EGKGDM 232 Query: 423 VIGISTDITERKQAEDELRQRESQLEKTVIQRTALLRELTNHLETVREEEKRAIARELHD 482 V G++ D+TER+Q+ +L + IQR LL ++ + V+E E++ +AR LHD Sbjct: 233 VAGLAIDVTEREQSRRDL--------ELAIQRQQLL---SSRVIEVQERERQHLARGLHD 281 Query: 483 NMGASLTALSMHLEGVYQIL----PPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTL 538 ++G SLTAL ++LE + ++ P + D ++ +++A R + +LRP L Sbjct: 282 DVGQSLTALKINLETIKKMGSLEGPSLQNGID-------IVTAVLAQVRSLSLDLRPPQL 334 Query: 539 DLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKA 598 D GL AA+ +++ + + D ++ H++E +R++QE + N+ +HA+ Sbjct: 335 DNLGLIAALRSYVEQKSKLANVNGWFESSGHDEQLHHDIENTAFRIVQEAVTNILRHARC 394 Query: 599 SKVDVILDIDEDHVALTVRDDGVGIPEER-RDNH---KTYGLRGLRERATFFGGEVDIRS 654 + V +D D V L++RDDG G ER R N ++GL + ERA GG ++I S Sbjct: 395 QNIWVKIDRQHDQVYLSIRDDGRGFDIERARSNAISGTSFGLLNMEERAVLVGGSMEITS 454 Query: 655 TPGKGALITISLPIRP 670 PG G I + LP+ P Sbjct: 455 APGAGTEIVMHLPVAP 470 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 89.7 bits (221), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 69/234 (29%), Positives = 114/234 (48%), Gaps = 16/234 (6%) Query: 456 ALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLEGVYQIL--PPDEKWADR-- 511 A LR+L + +EEE+ ++R+LHD + L ++ + +E L P++ R Sbjct: 233 AKLRQLAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAG 292 Query: 512 --KVRMQGLMKSLVATTRRIQTELRPNTLDLFGLKAAISEQLDELHERTGIAC-----HA 564 K Q + ++A RRI +LRP LD GL AA+ E + G + Sbjct: 293 FEKTATQ--INGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFENQ 350 Query: 565 SLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGIP 624 + D+ + + ++R+ QE L N+ +HA AS++ V L + + L V+D+G G Sbjct: 351 AQADDGATLPDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHGFD 410 Query: 625 EERRDNHKTY--GLRGLRERATFFGGEVDIRSTP-GKGALITISLPIRPELVPS 675 + D+H + GLR +RER GGE+ I S+P G I+ P L+P+ Sbjct: 411 LGQVDDHSRHGIGLRNMRERIEAVGGELGIDSSPAGTSISAVIARASLPSLIPA 464 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 70.1 bits (170), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 59/207 (28%), Positives = 103/207 (49%), Gaps = 8/207 (3%) Query: 472 EKRAIARELHDNMGASLTALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQT 531 E+ +A+ LHD++ L L++ ++ + L D R + L+++ V Sbjct: 446 ERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLDEIRDI--APLLRTGVQENYEDVR 503 Query: 532 ELRPN---TLDLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEM 588 EL N L L + + + LD+L ++G+ +L + + E ++ + +LQE Sbjct: 504 ELLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQITLTGDGPPLAPEQQLQVLFILQEA 563 Query: 589 LNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGIPEE--RRDNHKTYGLRGLRERATFF 646 L+N+ KHA+A +V + +D +D +TV DDG G E R + GLR ++ERA Sbjct: 564 LSNIRKHARAQQVQISVDNRQD-FRMTVSDDGHGFTLESARAKGLEHVGLRIMQERAERL 622 Query: 647 GGEVDIRSTPGKGALITISLPIRPELV 673 G ++I S PG G I ++L + LV Sbjct: 623 GANLEIDSQPGHGTRIALTLLRQERLV 649 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 60/212 (28%), Positives = 103/212 (48%), Gaps = 18/212 (8%) Query: 468 VREEEKRAIARELHDNMGASLTALSMHLEGVYQIL-------PPDEKWADRKVRMQGLMK 520 +RE+E+ IAR+LHD++G L + M L + + L P E R+ L++ Sbjct: 150 IREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQ-----LLQ 204 Query: 521 SLVATTRRIQTELRPNTLDLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIA 580 +A+ I EL P ++ FGL A+ Q ++ + C P + + + Sbjct: 205 QAMASMHSIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPTLEQEDRFLILG 263 Query: 581 IYRMLQEMLNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGI----PEERRDNHKTYGL 636 + R+L+E +N +H +AS + V L + + + + + DDG GI +E H +GL Sbjct: 264 LLRILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVAPRH-GHGL 322 Query: 637 RGLRERATFFGGEVDIRSTPGKGALITISLPI 668 R +RERA GGE+ I + G +I +P+ Sbjct: 323 RNMRERAQALGGELFIEAGQDGGVVIRTLIPV 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,831,660 Number of extensions: 68644 Number of successful extensions: 350 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 344 Number of HSP's successfully gapped: 6 Length of query: 677 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 585 Effective length of database: 1,184,250 Effective search space: 692786250 Effective search space used: 692786250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)