BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0307.p (234 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel f... 466 e-133 gi|300309940|ref|YP_003774032.1| biopolymer transport transmembr... 96 2e-21 gi|300310025|ref|YP_003774117.1| biopolymer transport transmembr... 90 1e-19 gi|300312127|ref|YP_003776219.1| biopolymer transport transmembr... 83 2e-17 gi|300310500|ref|YP_003774592.1| biopolymer transport ExbB-like ... 54 8e-09 >gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel family protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 466 bits (1198), Expect = e-133, Method: Compositional matrix adjust. Identities = 234/234 (100%), Positives = 234/234 (100%) Query: 1 MNVTQDLSFISLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFW 60 MNVTQDLSFISLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFW Sbjct: 1 MNVTQDLSFISLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFW 60 Query: 61 SGGNLSALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGA 120 SGGNLSALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGA Sbjct: 61 SGGNLSALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGA 120 Query: 121 RRAMRAAYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA 180 RRAMRAAYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA Sbjct: 121 RRAMRAAYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA 180 Query: 181 PGIAEALIATAIGLFAAIPAVVAYNRYSHDIDRLAIRFESFIEEFSNILQRQAR 234 PGIAEALIATAIGLFAAIPAVVAYNRYSHDIDRLAIRFESFIEEFSNILQRQAR Sbjct: 181 PGIAEALIATAIGLFAAIPAVVAYNRYSHDIDRLAIRFESFIEEFSNILQRQAR 234 >gi|300309940|ref|YP_003774032.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 95.9 bits (237), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 79/238 (33%), Positives = 109/238 (45%), Gaps = 23/238 (9%) Query: 5 QDLSFISLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFWSGGN 64 Q++ F A + V LLL SI SW YI K + R + ER FW Sbjct: 3 QNIGFAHYWAQGDAVSHSVAYLLLIMSIASWYYILSKAWAAWRIRRGSNALER-FWQAPT 61 Query: 65 LSALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAAR-------GGAVDPSLLL 117 L+ DA + ++ E+I+ M E A + R A DP L+ Sbjct: 62 LA----DATAAMQRLDS-----EQIYTP-MAERAAEAADIGTRQQGTPSLNAATDPGELI 111 Query: 118 DGARRAMRAAYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLA 177 R +R R LE L LASVGS +P+VGLFGTVWGI +A ++ + Sbjct: 112 T---RTLRREINRVSARLERGLTLLASVGSTAPFVGLFGTVWGIYHALVAVSASGTVQID 168 Query: 178 AVAPGIAEALIATAIGLFAAIPAVVAYNRYSHDIDRLAI-RFESFIEEFSNILQRQAR 234 VA + EALI TA+GL AIPAV+AYN ++ I+R+ + + F + L R Sbjct: 169 KVAGPVGEALIMTALGLLVAIPAVLAYNAFTR-INRVVLAELDGFAHDLHAYLTTGER 225 >gi|300310025|ref|YP_003774117.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/199 (34%), Positives = 93/199 (46%), Gaps = 19/199 (9%) Query: 11 SLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFWSGGNL----S 66 SL A + + V +LLL+ SI SW I K + R + FW NL + Sbjct: 10 SLWAQGDFVTKGVAVLLLAMSIASWYVIITKALQLLRLRKASAAAGHQFWDTTNLPEGVA 69 Query: 67 ALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGARRAMRA 126 L QD + +AG+ T A +G D + D ++R Sbjct: 70 TLGQD------------NPFAEVAKAGV---TAMSHHTAHKGHLHDQLSVSDWVTLSLRQ 114 Query: 127 AYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEA 186 A L++ +A LASVGS +P+VGLFGTVWGI +A + QA++ VA + EA Sbjct: 115 AIDEASGKLQAGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTTGQASIDKVAGPVGEA 174 Query: 187 LIATAIGLFAAIPAVVAYN 205 LI TA+GL AIPA YN Sbjct: 175 LIMTALGLAVAIPATFGYN 193 >gi|300312127|ref|YP_003776219.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 82.8 bits (203), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 62/197 (31%), Positives = 98/197 (49%), Gaps = 29/197 (14%) Query: 18 VLVQLVMLLLLSASIISW-TYIFRKMFTIRAARVQTEEFERVFWSGGNLSALYQDALSNR 76 V+ + ++ +LL S +SW I + M R +R+ + + FW+ ++ Sbjct: 15 VVTRFLITILLGMSSVSWIVMIVKGMANRRLSRIGLKAHKE-FWAAHSIE---------- 63 Query: 77 RKAGGAGGALERIFQAGMGEFTK-AKASVAARGGAVDPSLLLDGARR-------AMRAAY 128 G + I ++ A A+V A+ P LL+G+ +R Sbjct: 64 -------GGIAAIGAGTDNPYSNIATAAVEAQAQKDQP--LLEGSTSNTEWVAYCVRNEL 114 Query: 129 QREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALI 188 ++ L+ LA LAS+GS SP++GLFGTVWGI +A + QA+L VA + E+L+ Sbjct: 115 DEQIAHLQGGLAVLASIGSTSPFIGLFGTVWGIYHALIAIGASGQASLDHVAGPVGESLV 174 Query: 189 ATAIGLFAAIPAVVAYN 205 TA+GLF AIPAV+ YN Sbjct: 175 MTAVGLFVAIPAVLGYN 191 >gi|300310500|ref|YP_003774592.1| biopolymer transport ExbB-like transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 201 Score = 54.3 bits (129), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 56/216 (25%), Positives = 88/216 (40%), Gaps = 37/216 (17%) Query: 27 LLSASIISWTYIFRKMFTIRAARVQTEEF----ERVFWSGGNLSALYQDALSNRRKAGGA 82 LL ASII + ++ +R +R+ + RV+ SG ++ D L G Sbjct: 15 LLIASIIGLALVIERLVYLRRSRILPPDLLNQVVRVYQSG-RITDDVADKLEQNSPLG-- 71 Query: 83 GGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGARRAMRAAYQREMDA----LESH 138 + AG+ +D R M+ A + A LE Sbjct: 72 -----HVLAAGLRN--------------------VDAPREVMKEAIEEAGQAAAHRLERF 106 Query: 139 LAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGLFAAI 198 L L ++ S++P +GLFGTV G++ F N A +A GI+ AL T GL A+ Sbjct: 107 LTTLGTIASLAPLMGLFGTVVGMIEIFAS-QNAAGTNPAQLAHGISIALYNTGFGLAIAM 165 Query: 199 PAVVAYNRYSHDIDRLAIRFESFIEEFSNILQRQAR 234 PA+V Y + +D + E +F +I+ R Sbjct: 166 PALVFYRHFRALVDGFVVDMEQQAVKFVDIVHDVRR 201 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 658,371 Number of extensions: 26724 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 5 Length of query: 234 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 152 Effective length of database: 1,231,600 Effective search space: 187203200 Effective search space used: 187203200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)