BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0306.p (144 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310553|ref|YP_003774645.1| thioesterase [Herbaspirillum se... 296 5e-82 >gi|300310553|ref|YP_003774645.1| thioesterase [Herbaspirillum seropedicae SmR1] Length = 144 Score = 296 bits (758), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 142/144 (98%), Positives = 144/144 (100%) Query: 1 MSAASSPFDWKIRVYYEDTDTGGVVFYANYLKFFERARTEWLRSFGFQQQQLAATTGAIF 60 MSAASSPFDWKIRVYYEDTDTGGVVFYANYLKFFERARTEWLRSFGFQQQQLAATTGAIF Sbjct: 1 MSAASSPFDWKIRVYYEDTDTGGVVFYANYLKFFERARTEWLRSFGFQQQQLAATTGAIF 60 Query: 61 VVKNTNVDYLSPARLDDELRLTVVVEKLRNASMEFVQEAWRLPSGDGEPRLLARGSITVV 120 VVKNTNVDYLSPARLDDELRLTVVVEKLRNASM+F+QEAWRLPSGDGEPRLLARGSITVV Sbjct: 61 VVKNTNVDYLSPARLDDELRLTVVVEKLRNASMQFIQEAWRLPSGDGEPRLLARGSITVV 120 Query: 121 CVDQATFRPRPIPDELLTCLKKTT 144 CVDQATFRPRPIPDELLTCLKKTT Sbjct: 121 CVDQATFRPRPIPDELLTCLKKTT 144 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 457,310 Number of extensions: 17291 Number of successful extensions: 28 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 28 Number of HSP's successfully gapped: 1 Length of query: 144 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 67 Effective length of database: 1,255,275 Effective search space: 84103425 Effective search space used: 84103425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)