BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0295.p (449 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310562|ref|YP_003774654.1| composite two-component transcr... 767 0.0 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 120 2e-28 gi|300313022|ref|YP_003777114.1| two component sensor histidine ... 113 4e-26 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 103 3e-23 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 101 1e-22 >gi|300310562|ref|YP_003774654.1| composite two-component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 604 Score = 767 bits (1980), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/418 (99%), Positives = 416/418 (99%) Query: 1 VAHQIQVEQIRLLCGNLGSSVVPGILVALLIVYALGERAGLWPLLAWAGGVIVSKLIDFA 60 VAHQIQVEQIRLLCGNLGSSVVPGILVALLIVYALGERAGLWPLLAWAGGVIVSKLIDFA Sbjct: 17 VAHQIQVEQIRLLCGNLGSSVVPGILVALLIVYALGERAGLWPLLAWAGGVIVSKLIDFA 76 Query: 61 DARRILAMSITPQQIGAVRRQLMLLHGLDGAAWGALAAVALDTGSPGGSILAIGVLAGVA 120 DARRILAMSITPQQIGAVRR+LMLLHGLDGAAWGALAAVALDTGSPGGSILAIGVLAGVA Sbjct: 77 DARRILAMSITPQQIGAVRRRLMLLHGLDGAAWGALAAVALDTGSPGGSILAIGVLAGVA 136 Query: 121 GNSMSILSPVLPVFISFCIVELGMLAGWVWIMHDPSLQVLSAAALLYVASLLVQARNSAR 180 GNSMSILSPVLPVFISFCIVELGMLAGWVWIMHDPSLQVLSAAALLYVASLLVQARNSAR Sbjct: 137 GNSMSILSPVLPVFISFCIVELGMLAGWVWIMHDPSLQVLSAAALLYVASLLVQARNSAR 196 Query: 181 AAQAAITLRYENTELIRRLQHESERTQQALAQAEQANAAKSKFLAAASHDLRQPIHAQGL 240 AAQAAITLRYENTELIRRLQHESERTQQALAQAEQANAAKSKFLAAASHDLRQPIHAQGL Sbjct: 197 AAQAAITLRYENTELIRRLQHESERTQQALAQAEQANAAKSKFLAAASHDLRQPIHAQGL 256 Query: 241 FLEVLNTTPLDPMQRDVVAKLRRAADNCADMLHTLLDYSRIEAGVVEPQVTRFRVQALVG 300 FLEVLNTTPLDPMQRDVVAKLRRAADNCADMLHTLLDYSRIEAGVVEPQVTRFRVQALVG Sbjct: 257 FLEVLNTTPLDPMQRDVVAKLRRAADNCADMLHTLLDYSRIEAGVVEPQVTRFRVQALVG 316 Query: 301 RISQEMAAQAEAKGLVFRCRESRAVVSADASLTELILRNLVSNAIRYTDRGGILVACRQR 360 RISQEMAAQAEAKGLVFRCRESRAVVSADASLTELILRNLVSNAIRYTDRGGILVACRQR Sbjct: 317 RISQEMAAQAEAKGLVFRCRESRAVVSADASLTELILRNLVSNAIRYTDRGGILVACRQR 376 Query: 361 GGQAWLEVWDTGVGISAAQQQEIFEEFRQLGNPERDHRKGLGLGLAIAQGLARLQGIN 418 GGQAWLEVWDTG GISAAQQQEIFEEFRQLGNPERDHRKGLGLGLAIAQGLARLQG+ Sbjct: 377 GGQAWLEVWDTGAGISAAQQQEIFEEFRQLGNPERDHRKGLGLGLAIAQGLARLQGLT 434 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 80/246 (32%), Positives = 129/246 (52%), Gaps = 6/246 (2%) Query: 177 NSARAAQAAITLRYENTEL--IRRLQHESERTQQALAQAEQANAAKSKFLAAASHDLRQP 234 N +RA + R N L I + Q+A AE AN AKS+F+A SH+LR P Sbjct: 643 NESRAVAQEESERQTNLLLQEIEAHKQTDAELQRAKEAAESANLAKSRFVAGMSHELRTP 702 Query: 235 IHAQGLFLEVLNT-TPLDPMQRDVVAKLRRAADNCADMLHTLLDYSRIEAGVVEPQVTRF 293 +++ + ++L+ + P ++D + +RR+ ++ ++ LLD ++IEAG + Sbjct: 703 LNSILGYAQILHVDATIPPARKDAIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDEL 762 Query: 294 RVQALVGRISQEMAAQAEAKGLVFRCRES---RAVVSADASLTELILRNLVSNAIRYTDR 350 ++A + +I A QAE KGL FR +S +V AD IL NL+ NA+++TD Sbjct: 763 ALEAFLEQIVGMFAPQAEQKGLHFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDH 822 Query: 351 GGILVACRQRGGQAWLEVWDTGVGISAAQQQEIFEEFRQLGNPERDHRKGLGLGLAIAQG 410 GG+ + R A+ +V DTG+GI+ ++ IF F + G GLGLAI++ Sbjct: 823 GGVTLRLRYAREIAYFDVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRM 882 Query: 411 LARLQG 416 L + G Sbjct: 883 LVHIMG 888 >gi|300313022|ref|YP_003777114.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 113 bits (282), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 72/213 (33%), Positives = 114/213 (53%), Gaps = 12/213 (5%) Query: 212 QAEQANAAKSKFLAAASHDLRQPIHA----QGLFLEVLNTTPLDPMQRDVVAKLRRAADN 267 +A QAN AKS+FL+ SH+ R P++ E L +PL + + + Sbjct: 323 EALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREELGESPL----AQYAVAIHDSGNR 378 Query: 268 CADMLHTLLDYSRIEAGVVEPQVTRFRVQALVGRISQEMAAQAEAKGLVFRCR---ESRA 324 D+++ +L+ + +E V + ++ LVG+ QA+AKGL CR + Sbjct: 379 LLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQAQAKGLPLECRIGEDVPQ 438 Query: 325 VVSADASLTELILRNLVSNAIRYTDRGGILVACRQRGGQAW-LEVWDTGVGISAAQQQEI 383 ++ D + +L NL+SNA+RYTD G ++V + G Q + + DTG+GI+A+QQ +I Sbjct: 439 ILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGPQQLTMRIKDTGIGIAASQQNDI 498 Query: 384 FEEFRQLGNPERDHRKGLGLGLAIAQGLARLQG 416 FE+F Q + R ++G GLGL IAQ L L G Sbjct: 499 FEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMG 531 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 103 bits (257), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 80/239 (33%), Positives = 123/239 (51%), Gaps = 15/239 (6%) Query: 191 ENTELIRRLQHESERTQQALAQA----EQANAAKSKFLAAASHDLRQPIHAQGLFLEVLN 246 E T+L+ + + +TQ L + E+AN KS+FLA SH+LR P+++ + ++L Sbjct: 442 EQTQLLELQKDDLAKTQVVLVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLA 501 Query: 247 TTP---LDPMQRDVVAKLRRAADNCADMLHTLLDYSRIEAGVVEPQVTRFRVQALVGRIS 303 L Q + A ++ +++ +LD S++EAG ++ F + L +S Sbjct: 502 DNKQGNLSDEQVRFAQTISSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELS 561 Query: 304 QEMAAQAEAKGLVFRCRESRAV---VSADASLTELILRNLVSNAIRYTDRGGI-LVACRQ 359 A A+ KGL F+ V + D IL+NL+SNAI++T++G + L Sbjct: 562 ASFAITAQQKGLGFKVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIYPD 621 Query: 360 RGGQAWLEVWDTGVGISAAQQQEIFEEFRQLGNPERDHRK--GLGLGLAIAQGLARLQG 416 GG+ V DTG+GI Q Q IFE FRQ HRK G GLGL+I++ LA L G Sbjct: 622 AGGRIAFAVRDTGIGIGEDQHQFIFEAFRQADGST--HRKYGGTGLGLSISRDLAHLLG 678 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 71/232 (30%), Positives = 117/232 (50%), Gaps = 12/232 (5%) Query: 196 IRRLQHESERTQQALAQAEQANAAKSKFLAAASHDLRQPIHAQGLFLEVLNTTPLDPMQR 255 +RR + ++ + +A+ AN AKS FLA SH++R P++ E+L + L QR Sbjct: 324 VRRREEDALLATRLKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQR 383 Query: 256 DVVAKLRRAADNCADMLHTLLDYSRIEAGVVEPQVTRFRVQALVGRISQEMAAQAEAKGL 315 D + A +++ +LD+S+IE+G ++ ++ F LV +S + K + Sbjct: 384 DYADSIHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDV 443 Query: 316 VF----RCRESRAVVSADASLTELILRNLVSNAIRYTDRGGILVACRQRGGQAWLEVW-- 369 F C E+ +VV A L + +L NL+SNAI++T G + ++ R ++ W Sbjct: 444 QFDLHLDCPENLSVVGDPARLKQ-VLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRL 502 Query: 370 -----DTGVGISAAQQQEIFEEFRQLGNPERDHRKGLGLGLAIAQGLARLQG 416 DTG+GIS Q++F F Q + G GLGLAI++ L L G Sbjct: 503 FFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMG 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,079,306 Number of extensions: 38301 Number of successful extensions: 332 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 305 Number of HSP's successfully gapped: 24 Length of query: 449 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 361 Effective length of database: 1,203,190 Effective search space: 434351590 Effective search space used: 434351590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)