BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0293.p (304 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 499 e-142 gi|300311595|ref|YP_003775687.1| two component response regulato... 223 1e-59 gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 70 3e-13 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 63 3e-11 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 499 bits (1285), Expect = e-142, Method: Compositional matrix adjust. Identities = 250/255 (98%), Positives = 251/255 (98%) Query: 1 MLTFIEPTLFDKLRSAQALPSPGGVRLTIMQLCAQDNPDMAELIHLVQADPALSGRIIRI 60 MLTFIEPTLFDKLRSAQALPSPGGVRLTIM+LCAQDNPDMAELIHLVQADPALSGRIIRI Sbjct: 1 MLTFIEPTLFDKLRSAQALPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRI 60 Query: 61 VNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFA 120 VNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFA Sbjct: 61 VNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFA 120 Query: 121 MACSAQAVTSQLRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAPARLAMEQ 180 MACSAQAVTSQLRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAPAR AMEQ Sbjct: 121 MACSAQAVTSQLRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAPARRAMEQ 180 Query: 181 SLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAMAE 240 SLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAMAE Sbjct: 181 SLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAMAE 240 Query: 241 LGCGPAQQAQAGACR 255 LGCGPAQQAQA R Sbjct: 241 LGCGPAQQAQAALAR 255 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 223 bits (568), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 116/242 (47%), Positives = 162/242 (66%), Gaps = 2/242 (0%) Query: 1 MLTFIEPTLFDKLRSAQALPSPGGVRLTIMQLCAQDNPDMAELIHLVQADPALSGRIIRI 60 M T+++P F +++ ++ LP+P GVRL IMQ+C Q++ + EL+ VQ DP L+GR+I+I Sbjct: 1 MSTYVDPQTFREMQDSEVLPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQI 60 Query: 61 VNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFA 120 N+ N + R +ASV+++ LL+VGL AVRQ+ALA+SL E+ CA F AFW+ S A Sbjct: 61 ANIPNINKGRMVASVNVELLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTA 120 Query: 121 MACSAQAVTSQLRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALL-TRALDAPARLAME 179 MAC+ QA+ L+VAP EMFT GLLA IG+L LASA+P Y LL A DA R+ E Sbjct: 121 MACAGQALGDLLQVAPPAEMFTIGLLAGIGQLGLASARPKRYGELLLAGAKDAQLRV-QE 179 Query: 180 QSLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAMA 239 + FGF+HL +AAA+MQDW P +FC+AVLH+ P G RQ ++ L LA +A Sbjct: 180 RESFGFDHLALAAAMMQDWHFPRIFCEAVLHHRDPAASGLEEDDRQQKVTRLLELAGCVA 239 Query: 240 EL 241 ++ Sbjct: 240 DV 241 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 69.7 bits (169), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 52/201 (25%), Positives = 95/201 (47%), Gaps = 9/201 (4%) Query: 18 ALPSPGGVRLTIMQLCAQDNPDMAELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSM 77 ALPS V +++ ++ + E+ + AD LS +I+R+ N R I +V Sbjct: 12 ALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYYHVSRTIGTVD- 70 Query: 78 DSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFAMACSAQAVTSQLRVAPL 137 D++L++G VR L ++ +A + + L FW +S A A+ + + ++ Sbjct: 71 DAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKL--FWRYSLDTALVAKWLAKRGKLNS- 127 Query: 138 GEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAP---ARLAMEQSLFGFNHLNIAAAL 194 FT GL+ IG+L + + P AL + P RL +E+ FG++ ++ A L Sbjct: 128 DFAFTVGLMHAIGQLVMHAGMPE--QALQVDKVAGPLDARRLDVERKSFGYDFADVGAEL 185 Query: 195 MQDWKIPDLFCDAVLHYETPV 215 + W+ PD F + + P+ Sbjct: 186 ARRWRFPDEFAKVIEAFPQPL 206 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 62.8 bits (151), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 71/274 (25%), Positives = 112/274 (40%), Gaps = 29/274 (10%) Query: 28 TIMQLCAQDN-PDMA--ELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSMDSLLLVG 84 + QL A N D++ E+ + DPALS +++R+ N R I +V D+L ++G Sbjct: 19 VVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFFHASRTIGTVD-DALRILG 77 Query: 85 LQAVRQLALALSLAETRAPASCANFSLPAFWAHSFAMACSAQAVTSQLRVAPLGE----- 139 VR L L + A + L FW H+ A +A+ + + +GE Sbjct: 78 FIMVRNLVLGNGM--VAAFKNTRGMDLHQFWRHTLYTAVAARWLA--MHAGMVGEDNGDA 133 Query: 140 MFTTGLLANIGKLTLASAQPTGYSAL--LTRALDAPARLAMEQSLFGFNHLNIAAALMQD 197 +FT G++ IG+L L + + L + LD+ R E+ +GF++ +++A L Sbjct: 134 VFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSD-RAEREKEAWGFHYADVSAELAAI 192 Query: 198 WKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAMAELGCGPAQQAQAGACRGR 257 W PD A+ P + A R D +HL A + A A Sbjct: 193 WHFPDALVTALRSVPDP-----LGAGRFDRAAAWVHLGGWAARVAILSQDDENAQAT--- 244 Query: 258 DRAGAVAAGAARPAGAVPAGRPGLAGVDADHAHR 291 A + G PA P L D D A R Sbjct: 245 -----YPAAVGKKLGVDPAWMPLLVKHDPDKAER 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 799,915 Number of extensions: 28887 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 4 Length of query: 304 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 219 Effective length of database: 1,217,395 Effective search space: 266609505 Effective search space used: 266609505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)