BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0291.p (706 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 1409 0.0 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 211 2e-55 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 189 6e-49 gi|300313029|ref|YP_003777121.1| two component hybrid sensor his... 178 1e-45 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 155 1e-38 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 1409 bits (3647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 699/706 (99%), Positives = 703/706 (99%) Query: 1 MLLIVSETCFAVLAAVALAQGALPAWQKLVLAAIWGIHALTLLRLLRENARTAEKLVQAK 60 MLLIVSETCFAVLAAVALAQGALPAWQKLVLAAIWGIHALTLLRLLRENARTAEKLVQAK Sbjct: 18 MLLIVSETCFAVLAAVALAQGALPAWQKLVLAAIWGIHALTLLRLLRENARTAEKLVQAK 77 Query: 61 EAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIEDSAHALLG 120 EAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIEDSAHALLG Sbjct: 78 EAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIEDSAHALLG 137 Query: 121 IVNDVLDFSKIDVGKMDIAVVEFDLAEVVEASAEALSFKAREKRVRLLAHLDSRLPPRLV 180 IVNDVLDFSKIDVGKMDIAVVEFDLA+VVEASAEALSFKAREKRVRLLAHLDSRLPPRLV Sbjct: 138 IVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAEALSFKAREKRVRLLAHLDSRLPPRLV 197 Query: 181 GDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTGDHCRVRFEVRDSGIGMDEDTLRR 240 GDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVT DHCRVRFEVRDSGIGMDEDTLRR Sbjct: 198 GDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTSDHCRVRFEVRDSGIGMDEDTLRR 257 Query: 241 LFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFELGIPVTTG 300 LFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFELGIPVTTG Sbjct: 258 LFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFELGIPVTTG 317 Query: 301 PGMIRGEPPAADRPRRIALVEPHDASAAMLHEYLRSAALETLRFGSASDALAALTGPGAS 360 PGMIR EPPAADRPRRIALVEPHDASAAMLHEYLRSAALETLRFGSASDALAALTGPGAS Sbjct: 318 PGMIRSEPPAADRPRRIALVEPHDASAAMLHEYLRSAALETLRFGSASDALAALTGPGAS 377 Query: 361 ASGIEAVIVSTQIQDLDAVSVMARLQAERPALRRVLLDHGVHGLDTGSKPHHEVLQLPLR 420 ASGI+AVIVSTQ+QDLDAVSVMARLQAERPALRRVLLDHGVHGLDTGSKPHHEVLQLPLR Sbjct: 378 ASGIDAVIVSTQVQDLDAVSVMARLQAERPALRRVLLDHGVHGLDTGSKPHHEVLQLPLR 437 Query: 421 KQQLLRALGVTDSAAEAQTSPHARMAVPATPRQGHSGQRDILLVEDNVMNQKVAVYQLNA 480 KQQLLRALGVTDSAAEAQTSPHARMA PATPRQGHSGQRDILLVEDNVMNQKVAVYQLNA Sbjct: 438 KQQLLRALGVTDSAAEAQTSPHARMAAPATPRQGHSGQRDILLVEDNVMNQKVAVYQLNA 497 Query: 481 MGYAADIAANGLEALERLARHRYALVLMDCQMPLLDGYETTRRIRRMQGELAHTTIVAMT 540 MGYAADIAANGLEALERLARHRYALVLMDCQMPLLDGYETTRRIRRMQGELAHTTIVAMT Sbjct: 498 MGYAADIAANGLEALERLARHRYALVLMDCQMPLLDGYETTRRIRRMQGELAHTTIVAMT 557 Query: 541 ANAMAGDRERCLEAGMDDYLTKPLLRSQLAAMLAHYLPPSAASAGSAPAAPQLIDEDRLQ 600 ANAMAGDRERCLEAGMDDYLTKPLLRSQL+AMLAHYLPPSAASAGSAPAAPQLIDEDRLQ Sbjct: 558 ANAMAGDRERCLEAGMDDYLTKPLLRSQLSAMLAHYLPPSAASAGSAPAAPQLIDEDRLQ 617 Query: 601 ELFGQDRALAHEMLAMFVEHTSPLMQELQAHCQDQEPALDEVQALGHRLAGSCMNLGLDA 660 ELFGQDRALAHEMLAMFVEHTSPLMQELQAHCQDQEPALDEVQALGHRLAGSCMNLGLDA Sbjct: 618 ELFGQDRALAHEMLAMFVEHTSPLMQELQAHCQDQEPALDEVQALGHRLAGSCMNLGLDA 677 Query: 661 LGQLGREMEMAARQGDLAQTRTRAQACAATFAQLCQHLQASQTRGL 706 LGQLGREMEMAARQGDLAQTRTRAQACAATFAQLCQHLQASQTRGL Sbjct: 678 LGQLGREMEMAARQGDLAQTRTRAQACAATFAQLCQHLQASQTRGL 723 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 211 bits (537), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 119/277 (42%), Positives = 173/277 (62%), Gaps = 7/277 (2%) Query: 29 LVLAAIWGIHALTLLRLLR--ENARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMN 86 + L + G LT ++ R E+A A +L KE ADHA + FL+ +SHEIRTPMN Sbjct: 308 ISLVGLIGGFVLTWRQVRRREEDALLATRL---KEEADHANEAKSHFLAMVSHEIRTPMN 364 Query: 87 AVLGMAGLLRSTRLDTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLA 146 +LG++ LL + L ++Q ++A +I + L+ I+ND+LDFSKI+ GK+D+ +V F Sbjct: 365 GILGLSELLMESGLSSKQRDYADSIHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPV 424 Query: 147 EVVEASAEALSFKAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEV 206 +V+ ++ + K V+ HLD +VGDP R++Q+L NLL NA+KFT AG V Sbjct: 425 RLVQELSDLYRPSLQRKDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHV 484 Query: 207 LLRVQALQVTGDHCRVR--FEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLS 264 L + A Q R R F + D+GIG+ + L++LF PFAQADSS++R++GGTGLGL+ Sbjct: 485 QLSMSARQDESGPSRWRLFFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLA 544 Query: 265 ICKRLVELMGGSIGVESALGTGSCFWFELGIPVTTGP 301 I K LVELMGG+I +S G + F FE+ P + P Sbjct: 545 ISKNLVELMGGTIACQSIPGESTRFSFEILCPEASTP 581 Score = 98.6 bits (244), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 51/119 (42%), Positives = 73/119 (61%), Gaps = 2/119 (1%) Query: 461 ILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLARHRYALVLMDCQMPLLDGYET 520 IL+ ED +N+++ L G NGL+A+E + Y LVLMDC MP++DGY+ Sbjct: 644 ILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTGHYDLVLMDCMMPIMDGYQA 703 Query: 521 TRRIRRMQGE--LAHTTIVAMTANAMAGDRERCLEAGMDDYLTKPLLRSQLAAMLAHYL 577 T IR + L T I+A+TA+A+ GDR+RCL+AGMDDYL+KP + L + +L Sbjct: 704 TAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDYLSKPFTQVGLMQTVEKWL 762 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 189 bits (481), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 162/579 (27%), Positives = 268/579 (46%), Gaps = 111/579 (19%) Query: 42 LLRLLREN-ARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTR- 99 LL L +++ A+T LV+ + A + FL+ MSHE+RTP+N+ L +A LL + Sbjct: 446 LLELQKDDLAKTQVVLVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQ 505 Query: 100 --LDTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAEVVEASAEALS 157 L +Q FA+ I + + LL ++ND+LD SK++ GK+DI F L ++ + + + + Sbjct: 506 GNLSDEQVRFAQTISSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFA 565 Query: 158 FKAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTG 217 A++K + ++ +P R+ D R+ QIL NLL NA+KFT GEV LR+ Sbjct: 566 ITAQQKGLGFKVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRI----YPD 621 Query: 218 DHCRVRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSI 277 R+ F VRD+GIG+ ED + +F F QAD S R+YGGTGLGLSI + L L+GG I Sbjct: 622 AGGRIAFAVRDTGIGIGEDQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDI 681 Query: 278 GVESALGTGSCFWFELGIP-------------------------------VTTGPGMIRG 306 V S LG GS F L +P V T P + Sbjct: 682 RVTSRLGEGSI--FTLSLPPLYSSEQAVSNTAPEPTPAAAAAASTLQAAHVATAPDVRIN 739 Query: 307 EPPAADRPRR-------------------IALVEPHDASAAMLHEYLRSAALETLRFGSA 347 PAA P+R I ++E A A +L + R + + SA Sbjct: 740 RTPAAAAPQRLQEISIDDDRDRIETSSRLILVIEDDAAFAMILRDLAREMGFQCVVTHSA 799 Query: 348 SDALAALTGPGASASGIEAVIVSTQIQDLDAVSVMARLQAERPALRRVLLDHGVHGLDTG 407 ++ +AA A A+++ + D + V+ +++ P R + VH + Sbjct: 800 NEGVAA-----AEMYRPSAILMDMNLPDFSGLGVLDQIK-RNPKTRHI----PVHVVSVA 849 Query: 408 SKPHHEVLQ-------LPLRKQQLLRALGVTDSAAEAQTSPHARMAVPATPRQGHSGQRD 460 H + + P+++++L+ A+ ++ + G R Sbjct: 850 DYSHEAMERGAIGYALKPVQREELIGAIRKLEA-------------------KFLQGLRH 890 Query: 461 ILLVEDNV-----MNQKVAVYQLNAMGY--AADIAANGLEALERLARHRYALVLMDCQMP 513 +L+VED+ + Q +A +N +G AAD AL RL ++ + ++MD +P Sbjct: 891 VLVVEDDARQLDSIRQLLAGENINLVGVRTAAD-------ALARLRQNTFDCMVMDLNLP 943 Query: 514 LLDGYETTRRIRRMQGELAHTTIVAMTANAMAGDRERCL 552 L GY+ ++ Q +A ++ T +++ + E+ L Sbjct: 944 DLSGYQLLEKMAE-QEHVAFPPVIVYTGRSLSAEEEQNL 981 Score = 84.7 bits (208), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 55/141 (39%), Positives = 75/141 (53%), Gaps = 7/141 (4%) Query: 444 RMAVPATPRQGHSGQRDILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLARHR- 502 RM A R+ R IL+VED+V N L G +IA NG EAL+ L R + Sbjct: 1020 RMLKVARDRETVFEGRRILVVEDDVRNIFALSSVLEPKGMKVEIARNGREALQALQRSQD 1079 Query: 503 -----YALVLMDCQMPLLDGYETTRRIRRMQGELAHTTIVAMTANAMAGDRERCLEAGMD 557 LVLMD MP +DG R IR+ Q I+A+TA AM D+E+CL AG + Sbjct: 1080 KPDAPIDLVLMDIMMPEMDGLTAMREIRK-QAVWKRLPIIALTAKAMKDDQEKCLAAGAN 1138 Query: 558 DYLTKPLLRSQLAAMLAHYLP 578 DY+ KPL +L +++ ++P Sbjct: 1139 DYIAKPLDVEKLLSLVRVWMP 1159 >gi|300313029|ref|YP_003777121.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1524 Score = 178 bits (452), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 110/245 (44%), Positives = 151/245 (61%), Gaps = 1/245 (0%) Query: 54 EKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLRSTRLDTQQAEFAKAIED 113 LV A+ A+ A + FL+++SHEIRTPMN VLGM LL+ T+ Q ++ E Sbjct: 881 NNLVDARLKAEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPLPAQQDYLDKAEI 940 Query: 114 SAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAEVVEASAEALSFKAREKRVRLLAHLDS 173 SA +LL ++ND+LDFSK+D ++ + + F+L A L+ + V L LD Sbjct: 941 SAKSLLNLLNDLLDFSKMDAKQLQLDLQAFELERFGHNLAAVLAGSPPQAGVSLHIALDP 1000 Query: 174 RLPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQ-VTGDHCRVRFEVRDSGIG 232 +LP RLVGD R++Q+L+NL GNALKFTH G + + L + G +R EV D+GIG Sbjct: 1001 QLPRRLVGDAHRLQQVLVNLTGNALKFTHQGHIDVHFTRLDPLPGRTVALRVEVSDTGIG 1060 Query: 233 MDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVESALGTGSCFWFE 292 + D L +F F QA++S TR+YGGTGLGL+I K LV LMGG + V S G GS FWF+ Sbjct: 1061 IAADRLDDIFDAFTQAEASTTRRYGGTGLGLAISKHLVGLMGGQLQVSSEPGRGSRFWFD 1120 Query: 293 LGIPV 297 L + V Sbjct: 1121 LQLAV 1125 Score = 88.6 bits (218), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 62/143 (43%), Positives = 83/143 (58%), Gaps = 6/143 (4%) Query: 461 ILLVEDNVMNQKVAVYQLNAMG---YAADIAANGLEALERLARHRYALVLMDCQMPLLDG 517 ILLVEDN +N++VA L G AD + G+ ARH + LVLMD QMP +DG Sbjct: 1174 ILLVEDNPVNRQVASELLALSGAQVTCADSGSMGVAMACDPARH-FDLVLMDLQMPGMDG 1232 Query: 518 YETTRRIRRMQGELAHTTIVAMTANAMAGDRERCLEAGMDDYLTKPL-LRSQLAAMLAHY 576 E TRRIR Q EL I+AMTANA D++RC+EAGM ++ KP+ + + + +LAH Sbjct: 1233 IEATRRIR-GQYELRGLPILAMTANASPEDKQRCIEAGMAGHIAKPIDIATLIPVLLAHL 1291 Query: 577 LPPSAASAGSAPAAPQLIDEDRL 599 + A PQ + +DRL Sbjct: 1292 HHERPVPEAAPQAVPQDLADDRL 1314 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 155 bits (392), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 94/256 (36%), Positives = 150/256 (58%), Gaps = 6/256 (2%) Query: 42 LLRLLRENARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAVLGMAGLLR-STRL 100 LL+ + + +T +L +AKEAA+ A L + F++ MSHE+RTP+N++LG A +L + Sbjct: 660 LLQEIEAHKQTDAELQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATI 719 Query: 101 DTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAEVVEASAEALSFKA 160 + + I S LL +++ +LD +KI+ GKM +A E L +E + +A Sbjct: 720 PPARKDAIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQA 779 Query: 161 REKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQVTGDHC 220 +K + RLP + D R+RQIL+NLLGNA+KFT G V LR++ + Sbjct: 780 EQKGLHFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLRYAREIA--- 836 Query: 221 RVRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMGGSIGVE 280 F+V D+GIG+ +D R+F PF ++ ++ R+ GTGLGL+I + LV +MGG + V+ Sbjct: 837 --YFDVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQ 894 Query: 281 SALGTGSCFWFELGIP 296 S +G GS F ++ +P Sbjct: 895 SQVGQGSSFHLKIYLP 910 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,939,891 Number of extensions: 78935 Number of successful extensions: 604 Number of sequences better than 1.0e-08: 58 Number of HSP's gapped: 538 Number of HSP's successfully gapped: 62 Length of query: 706 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 614 Effective length of database: 1,184,250 Effective search space: 727129500 Effective search space used: 727129500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)