BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0277.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 526 e-151 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 132 4e-32 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 122 3e-29 gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase o... 116 2e-27 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 113 1e-26 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 526 bits (1356), Expect = e-151, Method: Compositional matrix adjust. Identities = 260/260 (100%), Positives = 260/260 (100%) Query: 1 MLLKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVA 60 MLLKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVA Sbjct: 1 MLLKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVA 60 Query: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT Sbjct: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 Query: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT Sbjct: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 Query: 181 NVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAA 240 NVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAA Sbjct: 181 NVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAA 240 Query: 241 FPTNALTGQSVVVSHGWHMQ 260 FPTNALTGQSVVVSHGWHMQ Sbjct: 241 FPTNALTGQSVVVSHGWHMQ 260 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 132 bits (331), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 85/258 (32%), Positives = 144/258 (55%), Gaps = 14/258 (5%) Query: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLA---LEAAKATAEEIVKEGGTAMAV 59 LKD+ ++T A GIG I + GA V I D++ L +A+A + G ++ Sbjct: 5 LKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARA------RFGHAPVSY 58 Query: 60 A-MNVTDETQVDKGVADAVNTYGG-LDIMISNAGIQ-IISPVAELSLENWRKMLAIHLDG 116 + +V+ E +VD AD + G LD++I+NAGI SPV E++L +W + LA++L G Sbjct: 59 SRTDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTG 118 Query: 117 AFLTTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKD 176 FL TR A+ + K G GG+I+ + SV L L+ PY +K+G++GL +T A E Sbjct: 119 PFLCTRRAVPLLKKNG-GGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPS 177 Query: 177 KIRTNVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAV 236 IR N + PG V E+ + +A +GI E++ ++++ + ++ + T +D+A + Sbjct: 178 HIRVNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLR-KMVTAEDIANQVI 236 Query: 237 FLAAFPTNALTGQSVVVS 254 FL + +++GQS+ V Sbjct: 237 FLCSPAGASISGQSLSVC 254 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 122 bits (307), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 87/255 (34%), Positives = 137/255 (53%), Gaps = 15/255 (5%) Query: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVAMN 62 L+ KVALITG+++GIG+ A+ +AR GA +++ +A + A I +GG A VA + Sbjct: 4 LQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGD 63 Query: 63 VTDETQVDKGVADAVNTYGGLDIMISNAG-IQIISPVAELSLENWRKMLAIHLDGAFLTT 121 V + ++GVA A++ +GGLDI I+NAG + P+AE+S + W+ L +L AFL Sbjct: 64 VGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGA 123 Query: 122 RAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRTN 181 R + AM+ +G G + V S P A Y AK GL+GL K +A + A IR N Sbjct: 124 RCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRAN 183 Query: 182 VICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAAF 241 + PG V T + P+Q K++ +K+I +++A A+FLA+ Sbjct: 184 ALLPGGVDTDMGGD--PQQ-KQWAAGLH-AMKRI----------AQPEEIAAAALFLASP 229 Query: 242 PTNALTGQSVVVSHG 256 + +TG ++ G Sbjct: 230 MASFVTGTALYADGG 244 >gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 708 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 87/263 (33%), Positives = 140/263 (53%), Gaps = 14/263 (5%) Query: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAM--AVA 60 L ++AL+TG GIG+ +A + +GA V++ D+ A + + ++K GG V Sbjct: 444 LAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQSLLKHGGPDAIGVVR 503 Query: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 ++T E V + +GG+D+++SNAGI SP+ + SLE W++ L++ G FL Sbjct: 504 ADITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLATGYFLV 563 Query: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 +RAA +M+ QG GGSI+Y+ S + +AS + Y TAK + LA+ +A EGA IR Sbjct: 564 SRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRV 623 Query: 181 NVICP-GFVRTPLVE--KQIPEQAKEFGISEEDV----IKKIMLKDTVDGEFTTTQDVAQ 233 NV+ P +R + K E+A I +DV ++ MLK +V +D+A+ Sbjct: 624 NVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSV-----LPEDIAE 678 Query: 234 TAVFLAAFPTNALTGQSVVVSHG 256 F A+ + TG + V G Sbjct: 679 AVYFFASEKSAKSTGNILNVDAG 701 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 73/252 (28%), Positives = 129/252 (51%), Gaps = 12/252 (4%) Query: 6 KVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVAMNVTD 65 +VAL+TG A GIG I+ + A +G V++AD+ L+AA TA+ +V G A + M++ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 66 ETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLTTRAAM 125 + A YG D++++NAG+ + LENW + + +++ G L + A Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 126 KAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRTNVICP 185 + M+KQ + G I+ + S+ A + Y T+K ++GL + +A E A+ I N + P Sbjct: 129 RLMVKQ-QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAP 187 Query: 186 GFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAAFPTNA 245 G V TP+ + EQ + E + + ++ + TT ++A +LAA + Sbjct: 188 GPVDTPMTQAMHSEQTR------ESYYRLVPMR-----RYGTTDEIASAVCYLAAEESAY 236 Query: 246 LTGQSVVVSHGW 257 +TG + V G+ Sbjct: 237 ITGHVIPVDGGF 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 704,783 Number of extensions: 25690 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 47 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)