BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0271.p (574 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310583|ref|YP_003774675.1| methyl-accepting chemotaxis I (... 1142 0.0 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 394 e-110 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 387 e-108 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 380 e-106 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 376 e-105 >gi|300310583|ref|YP_003774675.1| methyl-accepting chemotaxis I (serine chemoreceptor) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 574 Score = 1142 bits (2954), Expect = 0.0, Method: Compositional matrix adjust. Identities = 571/574 (99%), Positives = 571/574 (99%) Query: 1 MIQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREA 60 MIQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREA Sbjct: 1 MIQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREA 60 Query: 61 GSQLALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLTAASGQ 120 GSQLALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLTAASGQ Sbjct: 61 GSQLALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLTAASGQ 120 Query: 121 SKQLFPIVETIRQQVASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQEDMKAAHAEA 180 SKQLFPIVETIRQQVASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQEDMKAAHAEA Sbjct: 121 SKQLFPIVETIRQQVASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQEDMKAAHAEA 180 Query: 181 KDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIVRPLKRAGNIAETISRGDLTQDFHDHG 240 KDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIV PLKRAGNIAETISRGDLTQDFHDHG Sbjct: 181 KDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIVGPLKRAGNIAETISRGDLTQDFHDHG 240 Query: 241 RDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNRTESQASSLEET 300 RDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNRTESQASSLEET Sbjct: 241 RDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNRTESQASSLEET 300 Query: 301 ASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSIKESSSKIVDII 360 ASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSIKESSSKIVDII Sbjct: 301 ASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSIKESSSKIVDII 360 Query: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKGLISDSV 420 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKGLISDSV Sbjct: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKGLISDSV 420 Query: 421 EKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVNHAITQIDEITQ 480 EKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVNHAITQIDEITQ Sbjct: 421 EKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVNHAITQIDEITQ 480 Query: 481 QNAALVEQAAAAAESLQEQADVLARAVAVFRLKTAQAQGSIPTPPPVPAPPAPLRTVNAP 540 QNAALVEQAAAAAESLQEQADVLARAVAVFRLKTAQAQGSIPTPPPVPAPPAPLRTVN P Sbjct: 481 QNAALVEQAAAAAESLQEQADVLARAVAVFRLKTAQAQGSIPTPPPVPAPPAPLRTVNTP 540 Query: 541 AAVTATRPVASLPAAAPAKRKPAALARDDEFEEF 574 AAVTATRPVASLPAAAPAKRKPAAL RDDEFEEF Sbjct: 541 AAVTATRPVASLPAAAPAKRKPAALTRDDEFEEF 574 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 394 bits (1012), Expect = e-110, Method: Compositional matrix adjust. Identities = 262/527 (49%), Positives = 330/527 (62%), Gaps = 30/527 (5%) Query: 2 IQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMT-AALNMREA 60 ++ LKIG RLA GF +L+L ++ +G+WR+ + A++ MT AL Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATD--------DMTRIALKKERD 52 Query: 61 GSQLALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSP--AGRGKELLTAAS 118 Q A+++ T A +DA QAY F+KQI S + KE+ A + Sbjct: 53 AVQWHAAIKENGVRTFAVMKSDDDA-----FQAY--FQKQIDAQSARISQLQKEVEAAIT 105 Query: 119 -GQSKQLFPIVETIR--QQVASNNYFD---------AAQLLKSDFLPAHDKWMASVGALA 166 + K+LF V +R + + FD A ++ + + D + V A Sbjct: 106 DAEEKRLFDEVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYA 165 Query: 167 AFQQEDMKAAHAEAKDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIVRPLKRAGNIAET 226 FQ++ + A YR + +L +G + + LG +++TRSI RPL +A IAET Sbjct: 166 DFQKQVIDQTAAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAET 225 Query: 227 ISRGDLTQDFHDHGRDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDL 286 ++ GDLT RDE ++ AL+ M NL + V+ I AS EIA GN+DL Sbjct: 226 VAAGDLTSRIDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDL 285 Query: 287 SNRTESQASSLEETASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATM 346 S RTE+QA SLEETAS+MEQLTSTV+QNA+NARQAN+L SAS AT+GG VV VVATM Sbjct: 286 SARTEAQAGSLEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATM 345 Query: 347 GSIKESSSKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSA 406 G+I SS KI DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA Sbjct: 346 GAIHASSQKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA 405 Query: 407 AAAKEIKGLISDSVEKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIA 466 +AAKEIK LI DSVEKVD G +LV AG TM E+V SV V ++ EIT ASQEQS GI Sbjct: 406 SAAKEIKQLIDDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIE 465 Query: 467 EVNHAITQIDEITQQNAALVEQAAAAAESLQEQADVLARAVAVFRLK 513 +VN AIT IDE TQQNAALVEQAAAAA S+Q+QA L AV+VFRL+ Sbjct: 466 QVNLAITHIDETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLE 512 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 387 bits (993), Expect = e-108, Method: Compositional matrix adjust. Identities = 258/523 (49%), Positives = 333/523 (63%), Gaps = 30/523 (5%) Query: 5 LKIGSRLAFGFCLILLLATAILLIGLWRMAELE---AASEYVIDEKVAAMTAALNMREAG 61 L I +LA F +++ L LL GL+ + EL+ AS ++ + + M AA ++ G Sbjct: 7 LNIAKKLALSFAVVIALT---LLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ--G 61 Query: 62 SQLALALRKV-----VTPTD-AAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLT 115 S L L ++ VTP + AA KA L + + A FE++++E + + L Sbjct: 62 SLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL- 120 Query: 116 AASGQSKQLFPIVETIRQ--QVASNNYFDAAQ-LLKSDFLPAHDKWMASVGALAAFQQED 172 K L V+ Q ++A+N +D + L D + + ++ A+ Sbjct: 121 ------KNLTAYVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSG 174 Query: 173 MKAAHAEAKDSYRKAQ---IGMLLMGVLTLGLGFFFTFVITRSIVRPLKRAGNIAETISR 229 A EA +R AQ +G+L VL L F++ ++ RPLK A IA+ ++R Sbjct: 175 SAQADEEASTVFRGAQRWIVGLLAATVLIASL---LAFLVATNVSRPLKEAVQIAQRVAR 231 Query: 230 GDLTQDFHDHGRDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNR 289 GDLT GRDE ++ AL AM +L ++EV++ I ASHEIARGN+DLSNR Sbjct: 232 GDLTLRIRPAGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNR 291 Query: 290 TESQASSLEETASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSI 349 TE QA SLEETAS+ME+LTSTV QNA+NARQANQL +ASD A++GG VVG VV TM I Sbjct: 292 TEQQAGSLEETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGI 351 Query: 350 KESSSKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAA 409 ESS +I DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA AA Sbjct: 352 TESSRRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAA 411 Query: 410 KEIKGLISDSVEKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVN 469 KEIK LI DSV+KV AG LV+ AG+TM+E+V SV+ V D++ EI+AA+QEQS GI EVN Sbjct: 412 KEIKELIDDSVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVN 471 Query: 470 HAITQIDEITQQNAALVEQAAAAAESLQEQADVLARAVAVFRL 512 AITQ+DE TQQNAALVEQAAAAA+SL+ QA LA V F+L Sbjct: 472 LAITQMDETTQQNAALVEQAAAAADSLKGQASNLAAVVGKFKL 514 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 380 bits (976), Expect = e-106, Method: Compositional matrix adjust. Identities = 249/551 (45%), Positives = 348/551 (63%), Gaps = 8/551 (1%) Query: 5 LKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREAGSQL 64 L I +RLA F +++L+ I+ +GL R+A++ A + V++++ + + + Sbjct: 6 LSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKVDDV 65 Query: 65 ALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLTAASGQSKQL 124 A++LR + TD A+ AE + + ++T L K L + K Sbjct: 66 AISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTETRKSY 125 Query: 125 FPIVETIRQQVASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQEDMKAAHAEAKDSY 184 + + I + +S + A +L+ S+ +P + + + L+ QQ+ M + +A+ +Y Sbjct: 126 AALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQAEAAY 185 Query: 185 RKAQIGMLLMGVLTLGLGFFFTFVITRSIVRPLKRAGNIAETISRGDLTQDFHDHGRDEA 244 ++ M++ GVL F + I+RSI RPL RA +AET++ GDLT H DE Sbjct: 186 ETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAHSNDET 245 Query: 245 ATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNRTESQASSLEETASSM 304 ++ AL AM L A + EV+ + AS +IA GN+DLS+RTE QAS+LEETASS+ Sbjct: 246 GRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEETASSL 305 Query: 305 EQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSIKESSSKIVDIIGVID 364 E+LTS+V+QNA++ R++++L +AS A +GG V VV TMG+I +SS KIVDII VID Sbjct: 306 EELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDIISVID 365 Query: 365 GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKGLISDSVEKVD 424 GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA AAKEIK LI+DSV +V Sbjct: 366 GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELINDSVNQVT 425 Query: 425 AGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVNHAITQIDEITQQNAA 484 +G +LV +AG+TM +V S++ V+ I+GEI+AA++EQS GI +VN AI Q+D TQQNAA Sbjct: 426 SGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMDNTTQQNAA 485 Query: 485 LVEQAAAAAESLQEQADVLARAVAVFRLKTAQAQGSIPTPPPVPAPPAP-LRTVNAPAAV 543 LVE+AAAAA++LQ+QA L V VFRL + GS P P V AP AP LR V+A A Sbjct: 486 LVEEAAAAAQALQDQAQRLLDLVGVFRLD--EVGGSGPAPSAV-APVAPVLRKVSAAAP- 541 Query: 544 TATRPVASLPA 554 R SLPA Sbjct: 542 ---RATKSLPA 549 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 376 bits (966), Expect = e-105, Method: Compositional matrix adjust. Identities = 267/572 (46%), Positives = 354/572 (61%), Gaps = 34/572 (5%) Query: 2 IQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREAG 61 I +KI RL F ++++ I +G+ + + A++ +++++ + + + Sbjct: 4 ISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITDKV 63 Query: 62 SQLALALRKVVTPTDAAEGKAE----DAHLSKILQAYAGFEKQITELSPAGRGKELLTA- 116 + A +LR + + E K D + S++ + A EK+ +P RG ELL A Sbjct: 64 NTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTN--TP--RGSELLAAL 119 Query: 117 ---ASGQSKQLFPIVETIRQQV---ASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQ 170 + SK+ +V+ I QQ A+ + FD +P DK+ A+V + +FQ Sbjct: 120 KQARADYSKERIKVVDLIHQQKRDEATAHLFDKT-------IPVQDKYFAAVKEMVSFQS 172 Query: 171 EDM-KAAHAEAKDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIVRPLKRAGNIAETISR 229 M K+A + S I ++L GV L L +ITRSI RPL A +A ++ Sbjct: 173 SLMDKSAERASAASSSAINIMLVLSGVAVL-LSALCAMLITRSITRPLNEAVQVATAVAA 231 Query: 230 GDLTQDFHDHGRDEAATMVNALEAMQTNLAATLNEVKESAAVIAVASHEIARGNVDLSNR 289 GDLT +DE ++ AL++M NL + EV+ I AS EIA GN+DLS+R Sbjct: 232 GDLTVQIEATSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSR 291 Query: 290 TESQASSLEETASSMEQLTSTVQQNAENARQANQLVVSASDYATKGGRVVGDVVATMGSI 349 TE QA +LEETAS+ME+LTSTV+QNA+NARQAN L +AS A +GG VVG+VV TMG I Sbjct: 292 TEQQAGALEETASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQI 351 Query: 350 KESSSKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAA 409 ++S KIVDII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSAAAA Sbjct: 352 NDASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAA 411 Query: 410 KEIKGLISDSVEKVDAGGKLVDEAGATMSEIVTSVKHVADIMGEITAASQEQSAGIAEVN 469 KEIK LI SVE+V++G +LV +AGATM E+V SVK V D++ EITAAS EQS GI ++N Sbjct: 412 KEIKSLIDASVERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQIN 471 Query: 470 HAITQIDEITQQNAALVEQAAAAAESLQEQADVLARAVAVFRLKTAQAQGSIPTPPPVPA 529 AI Q+DE+TQQNAALVEQAAAAA+SLQEQ+ L+ V +FRL A P P+ A Sbjct: 472 QAIVQMDEVTQQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAH----QPSPLSA 527 Query: 530 PPAPLRTVN-APA-AVTATRPVASLPAAAPAK 559 P R+V APA V A +PV +APAK Sbjct: 528 PVQ--RSVQVAPARPVLARKPVTR--TSAPAK 555 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,064,301 Number of extensions: 32833 Number of successful extensions: 489 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 273 Number of HSP's successfully gapped: 71 Length of query: 574 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 484 Effective length of database: 1,193,720 Effective search space: 577760480 Effective search space used: 577760480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)