BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0270.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310584|ref|YP_003774676.1| methyl-accepting chemotaxis tra... 1181 0.0 gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sen... 379 e-106 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 370 e-103 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 355 5e-99 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 352 4e-98 >gi|300310584|ref|YP_003774676.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 596 Score = 1181 bits (3056), Expect = 0.0, Method: Compositional matrix adjust. Identities = 593/596 (99%), Positives = 594/596 (99%) Query: 1 MSIKNLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLSQERLPKTVAADNTLI 60 MSIKNLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLSQERLPKTVAADNTLI Sbjct: 1 MSIKNLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLSQERLPKTVAADNTLI 60 Query: 61 WIGRQRTSLDQAALTNDPAWAERMYGMDANARKEALQWWGKYTALPQTEEGRVLVKKVSD 120 WIGRQRTSLDQAALTNDPAWAERMYGMDANARKEALQWWGKYTALPQTEEGRVLVKKVSD Sbjct: 61 WIGRQRTSLDQAALTNDPAWAERMYGMDANARKEALQWWGKYTALPQTEEGRVLVKKVSD 120 Query: 121 GIDKTEQELTRFAEVIKAGDRQAIADQARKVGTIYTAMQLDGQALSKYETEQAAQSAGAS 180 GIDKTEQELTRFAEVIKAGDRQAIADQARKVGTIYTAMQLDGQALSKYETEQAAQSAGAS Sbjct: 121 GIDKTEQELTRFAEVIKAGDRQAIADQARKVGTIYTAMQLDGQALSKYETEQAAQSAGAS 180 Query: 181 MGRYEASRNFSIVAIVAGLLLAFYSWWSLRRAIGEPMQRALRHFDAIASGDLTEAVHVHS 240 M RYEASRNFSIVAIVAGLLLAFYSWWSLRRAIGEPMQRALRHFDAIASGDLTEAVHVHS Sbjct: 181 MARYEASRNFSIVAIVAGLLLAFYSWWSLRRAIGEPMQRALRHFDAIASGDLTEAVHVHS 240 Query: 241 RNEMGQLLEGLKRMQESLSRTVQAVRSGSEAISTATREIASGNLDLSSRTEQQAASLEET 300 RNEMGQLLEGLKRMQESLSRTVQAVRSGS+AISTATREIASGNLDLSSRTEQQAASLEET Sbjct: 241 RNEMGQLLEGLKRMQESLSRTVQAVRSGSDAISTATREIASGNLDLSSRTEQQAASLEET 300 Query: 301 ASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGRVVQTMEEIRASSAKITDIV 360 ASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGRVVQTMEEIRASSAKITDIV Sbjct: 301 ASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGRVVQTMEEIRASSAKITDIV 360 Query: 361 SIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSSAAKEIKELIMHSV 420 SIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSSAAKEIKELIMHSV Sbjct: 361 SIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSSAAKEIKELIMHSV 420 Query: 421 DRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQSAGIDQVSHAVTQMDEATQ 480 DRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQSAGIDQVSHAVTQMDEATQ Sbjct: 421 DRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQSAGIDQVSHAVTQMDEATQ 480 Query: 481 QNAALVEQASAAAKSLEQQAQALMQEVAIFRVNGAGPTVKPPAAAASRATVKPAAKPAMK 540 QNAALVEQASAAAKSLEQQAQALMQEVAIFRVNGAGPTVKPPAAAASRATVKPAAKPAMK Sbjct: 481 QNAALVEQASAAAKSLEQQAQALMQEVAIFRVNGAGPTVKPPAAAASRATVKPAAKPAMK 540 Query: 541 PASAAPAAPATPTAPAAPVAKVAPAVKRPALPAAPAAPKPAGRGVPAGDEGDWETF 596 PASAAPAAPATPTAPAAPVAKVAPAVKRPALPAAPAA KPAGRGVPAGDEGDWETF Sbjct: 541 PASAAPAAPATPTAPAAPVAKVAPAVKRPALPAAPAAAKPAGRGVPAGDEGDWETF 596 >gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sensory transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 379 bits (973), Expect = e-106, Method: Compositional matrix adjust. Identities = 225/407 (55%), Positives = 285/407 (70%), Gaps = 28/407 (6%) Query: 196 VAGLLLAFYSWWSLRRAIGEPMQRALRHFDAIASGDLTEAVHVHSRNEMGQLLEGLKRMQ 255 +A +L +WW L I P+ A++ +ASGDLT + V SR+E G LL LK M Sbjct: 201 IALMLAVLLAWW-LANNITRPLALAVQLARRVASGDLTARIEVRSRDETGALLGALKDMN 259 Query: 256 ESLSRTVQAVRSGSEAISTATREIASGNLDLSSRTEQQAASLEETASSMEELTSTVKHNA 315 ESL R V VR G+E I+TA+ EIA+GNLDLSSRTEQQA+SLEETASSMEELTSTV+ N Sbjct: 260 ESLVRIVSGVRRGTEMIATASSEIATGNLDLSSRTEQQASSLEETASSMEELTSTVQQNT 319 Query: 316 DNARQATTLAVNASTIAADGNAVVGRVVQTMEEIRASSAKITDIVSIIDGIAFQTNILAL 375 NAR+A +A AS IA+ G VV RVV TMEEI SS KI DI+S+IDGIAFQTNILAL Sbjct: 320 ANAREANRMAQAASDIASSGGGVVARVVDTMEEINDSSRKIVDIISVIDGIAFQTNILAL 379 Query: 376 NAAVEAARAGEQGRGFAVVATEVRALAQRSSSAAKEIKELIMHSVDRVQAGSELVGQAGD 435 NAAVEAARAGEQGRGFAVVA+EVR+LAQRS++AAKEIK LI SV +V++GS LV +AGD Sbjct: 380 NAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKALISDSVAKVESGSLLVNEAGD 439 Query: 436 TMSDVINAVQRVTDIMSEISAASNEQSAGIDQVSHAVTQMDEATQQNAALVEQASAAAKS 495 TM +++ +VQRVT++++EISAA EQ +GI+QVS AV QMD+ TQQNAALVEQA+AA++S Sbjct: 440 TMREIVESVQRVTNMINEISAAGEEQRSGIEQVSQAVGQMDQVTQQNAALVEQAAAASQS 499 Query: 496 LEQQAQALMQEVAIFRVNGA-----GPTVKPPAAAASRATVKPAAKPAMKPASAAPAAPA 550 L++QAQ L Q V++FR+ P P + AASR+ V+PA + + P+ P+ A Sbjct: 500 LQEQAQELAQAVSVFRLEAGQALEVAPEPAPASVAASRSGVRPATRSSRLPSPVGPS--A 557 Query: 551 TPTAPAAPVAKVAPAVKRPALPAAPAAPKPA-GRGVPAGDEGDWETF 596 +P+ A+P P PA G + + D +W TF Sbjct: 558 SPS-------------------ASPMHPAPASGSRLSSADADEWVTF 585 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 370 bits (949), Expect = e-103, Method: Compositional matrix adjust. Identities = 249/537 (46%), Positives = 344/537 (64%), Gaps = 30/537 (5%) Query: 3 IKNLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLSQERLPKTVAADNTLIWI 62 KNL + +LA + ALL + + + ++ Q+ +R K A + + Sbjct: 5 FKNLKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGV 64 Query: 63 GRQRTSLDQAALT--NDPAWAERMYGMDANAR--KEALQWWGKYTALPQTEEGRVLVKKV 118 Q L A+ N PA +R ++ + KEAL K TA T G+ K + Sbjct: 65 NEQIKYLRGIAIDIENRPANEKRFAQLEVAVKQTKEALD---KITARQVTAVGQKKAKAL 121 Query: 119 SDGIDKTEQELTRFAEVIKAGDRQAIADQA--RKVGTIYTAMQLDGQALSK--------- 167 + + F ++KAG+ + AD+ RK+ T Q + AL++ Sbjct: 122 DEARIAFDASKADFLALVKAGNSTS-ADEYVLRKI----TDTQNNYLALARAFADSQDAQ 176 Query: 168 YETEQAAQSAGASMGRYEASRNFSIVAIVAGLLLAFYSWWSLRRAIGEPMQRALRHFDAI 227 + E A A +M E + S+ A++A ++L + + R+I +P+ A+R + + Sbjct: 177 LQAEGAKAVADGAMA-IEVTLACSMAAVLAAIILGYL----MARSIVKPLTEAVRVAENV 231 Query: 228 ASGDLTEAVHVHSRNEMGQLLEGLKRMQESLSRTVQAVRSGSEAISTATREIASGNLDLS 287 A+GDLT + HSR+E GQL++ L++M ++L V VR G+E+I+TA+ EIASGNLDLS Sbjct: 232 AAGDLTTRIEPHSRDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLS 291 Query: 288 SRTEQQAASLEETASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGRVVQTME 347 +RTEQQA SLEETAS+MEE+TSTV+ NADNARQA +AV+AS IA G +VGRVV TM+ Sbjct: 292 ARTEQQAGSLEETASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMD 351 Query: 348 EIRASSAKITDIVSIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSS 407 EI SS KI DI+ +IDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVR+LAQRS++ Sbjct: 352 EINQSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAA 411 Query: 408 AAKEIKELIMHSVDRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQSAGIDQ 467 AAKEIK LI SV +V+ GS LV QAG TM V+++V+ VTDI++EISAAS EQS+GI++ Sbjct: 412 AAKEIKALIADSVGKVEGGSALVAQAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEE 471 Query: 468 VSHAVTQMDEATQQNAALVEQASAAAKSLEQQAQALMQEVAIFRVNGAGPTVKPPAA 524 ++ AVTQMDE TQQNAALVEQA+AAAKS+++QA L Q ++IF++ ++ PAA Sbjct: 472 INRAVTQMDEGTQQNAALVEQAAAAAKSMQEQAVRLTQSISIFKLEQGAASI--PAA 526 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 355 bits (912), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 249/564 (44%), Positives = 348/564 (61%), Gaps = 23/564 (4%) Query: 1 MSIKNLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLS---QERLPKTVAADN 57 M NLSI +LAIA +L ++ I A LG++ IN L +R K +A N Sbjct: 1 MRFNNLSIATRLAIAFSILILVIAGIVA---LGLARLADINKALDLVVNDRYKK-IAIIN 56 Query: 58 TLIW-IGRQRTSLDQAALTNDPAWAERMYGMDANARKEALQWWGKYTALPQTEEGRVLVK 116 T+ + SL L DPA + E + + + T L E + + Sbjct: 57 TISGKVDDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLD 116 Query: 117 KVSDGIDKTEQELTRFAEVIKAGDRQA----IADQARKVGTIYTAMQLDGQALSKYETEQ 172 KV++ + ++ +GD+ A + + + IY + QLD LS + + Sbjct: 117 KVTETRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFS-QLD--ELSDTQQKL 173 Query: 173 AAQSAGASMGRYEASRNFSIVAIVAGLLLAFYSWWSLRRAIGEPMQRALRHFDAIASGDL 232 QS + YE +R ++A V A + W++ R+I P+ RA+ + +A+GDL Sbjct: 174 MDQSVDQAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDL 233 Query: 233 TEAVHVHSRNEMGQLLEGLKRMQESLSRTVQAVRSGSEAISTATREIASGNLDLSSRTEQ 292 T + HS +E G+LL+ L M + L V VR G++++ TA+ +IA+GNLDLSSRTE+ Sbjct: 234 TAVIAAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEE 293 Query: 293 QAASLEETASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGRVVQTMEEIRAS 352 QA++LEETASS+EELTS+VK NAD+ R+++ LA NAS +A G V +VVQTM I S Sbjct: 294 QASALEETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDS 353 Query: 353 SAKITDIVSIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRALAQRSSSAAKEI 412 S KI DI+S+IDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LAQRS++AAKEI Sbjct: 354 SHKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEI 413 Query: 413 KELIMHSVDRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQSAGIDQVSHAV 472 KELI SV++V +G+ELV QAG TM V+N++++V+ I+ EISAA+ EQS GI+QV+ A+ Sbjct: 414 KELINDSVNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAI 473 Query: 473 TQMDEATQQNAALVEQASAAAKSLEQQAQALMQEVAIFRVNGAGPTVKPPAAAASRATVK 532 QMD TQQNAALVE+A+AAA++L+ QAQ L+ V +FR++ G + P+A A Sbjct: 474 MQMDNTTQQNAALVEEAAAAAQALQDQAQRLLDLVGVFRLDEVGGSGPAPSAVA------ 527 Query: 533 PAAKPAMKPASAAPAAPATPTAPA 556 P A P ++ SAA A AT + PA Sbjct: 528 PVA-PVLRKVSAA-APRATKSLPA 549 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 352 bits (904), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 266/615 (43%), Positives = 353/615 (57%), Gaps = 37/615 (6%) Query: 1 MSIKNLSIKAQLAIAILVLAALLVAIGASGLLGMSAAVSINAQLSQERLPKTVAADNTLI 60 M +K++ I +LA+ VL ++ I G+ +S+ +A + ++R K A Sbjct: 1 MKLKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRD 60 Query: 61 WIGRQRTSLDQAALTNDPAWAERMYGMDANARKEALQWWGKYTALPQTEEGRVLVKKVSD 120 + +L A L + A R A + + GK + T GR L + + + Sbjct: 61 NVNGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQE 120 Query: 121 GI-------DKTEQELTR-----------FAEVIKAGDRQ-AIADQARKVGTIYTAMQLD 161 DK +EL + F +VI A D+ + DQ + A Q Sbjct: 121 ARSAYNTPRDKL-RELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQ-------FVAFQKS 172 Query: 162 GQALSKYETEQAAQSAGASMGRYEASRNFSIVAIVAGLLLAFYSWWSLRRAIGEPMQRAL 221 S E QSA A M S VAI L + W + R+I P+ A+ Sbjct: 173 LMEQSVTEGRDTTQSAIAMM------LGLSAVAIA----LCVIAAWLVTRSITRPLNEAV 222 Query: 222 RHFDAIASGDLTEAVHVHSRNEMGQLLEGLKRMQESLSRTVQAVRSGSEAISTATREIAS 281 A+A+GDLT + S++E G LL LK M +SL R V VR GS+ I+TA+ EIA+ Sbjct: 223 SVASAVAAGDLTVQIGATSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIAT 282 Query: 282 GNLDLSSRTEQQAASLEETASSMEELTSTVKHNADNARQATTLAVNASTIAADGNAVVGR 341 GNLDLSSRTE+QA +LEETAS+MEELTSTVK NADNARQA +LA AS +A G +VVG+ Sbjct: 283 GNLDLSSRTEEQAGALEETASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQ 342 Query: 342 VVQTMEEIRASSAKITDIVSIIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRAL 401 VVQTM EI +S KI DI+S+IDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR L Sbjct: 343 VVQTMGEINDASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTL 402 Query: 402 AQRSSSAAKEIKELIMHSVDRVQAGSELVGQAGDTMSDVINAVQRVTDIMSEISAASNEQ 461 AQRS+SAAKEIK LI SV RV GS LV QAG TM++V+ +V+RVTD+++EISAASNEQ Sbjct: 403 AQRSASAAKEIKALIDDSVSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQ 462 Query: 462 SAGIDQVSHAVTQMDEATQQNAALVEQASAAAKSLEQQAQALMQEVAIFRVNGAGPTVKP 521 S GI+Q++HA+TQMD+ TQQNAALVE+A+AAA+SL++Q+ L V++F+++ Sbjct: 463 SDGIEQINHAITQMDQVTQQNAALVEEAAAAAQSLQEQSTRLSATVSVFKLSSHDTPHAA 522 Query: 522 PAAAASRATVKPAAKPAMKPASAAPAAPATPTAPAAPVAKVAPAVKRPALPAAPAAPKPA 581 + A A +A A + A+P + + K A A A Sbjct: 523 APRKPAPAQAASRPAAAATVRTAPSKPAAVLASSASPASPASTTPKALPSKPAAPASAKA 582 Query: 582 GRGVPAGDEGDWETF 596 G A D+GDWE F Sbjct: 583 GSKAMAADDGDWEQF 597 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.123 0.329 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,301,710 Number of extensions: 45304 Number of successful extensions: 696 Number of sequences better than 1.0e-08: 71 Number of HSP's gapped: 379 Number of HSP's successfully gapped: 73 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)