BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0261.p (431 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310593|ref|YP_003774685.1| phosphate regulon two-component... 875 0.0 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 115 8e-27 gi|300310697|ref|YP_003774789.1| two component hybrid sensor his... 107 2e-24 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 107 2e-24 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 104 1e-23 >gi|300310593|ref|YP_003774685.1| phosphate regulon two-component sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 436 Score = 875 bits (2261), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/431 (99%), Positives = 428/431 (99%) Query: 1 LFWIPAALRLVLCLAGAGVVGFFFGPIAGLVTAVLGVGAMMITHLHYLLRLSTWLDDSGQ 60 LFWIPA LRLVLCLAGAGVVGFFFGPIAGLVTAVLGVGAMMITHLHYLLRLSTWLDDSGQ Sbjct: 6 LFWIPAVLRLVLCLAGAGVVGFFFGPIAGLVTAVLGVGAMMITHLHYLLRLSTWLDDSGQ 65 Query: 61 SRLPDGWGAWTDVYARLYRMRRDDEKNQAELAEWLARFRQAMSLLPDGVVIMDDVLFLEW 120 SRLPDGWGAWTDVYARLYRMRRDDEKNQAELAEWLARFRQAMSLLPDGVVIMDDVLFLEW Sbjct: 66 SRLPDGWGAWTDVYARLYRMRRDDEKNQAELAEWLARFRQAMSLLPDGVVIMDDVLFLEW 125 Query: 121 CNPAAQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLTMTLHERKLIIQIIPFE 180 CNPAAQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLTMTLHERKLIIQIIPFE Sbjct: 126 CNPAAQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLTMTLHERKLIIQIIPFE 185 Query: 181 NRRQILVTHDVTESERIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLEEGTRMAH 240 NRRQILVTHDVTESERIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLEEGTRMAH Sbjct: 186 NRRQILVTHDVTESERIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLEEGTRMAH 245 Query: 241 LKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAGKHEIV 300 LKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAGKHEIV Sbjct: 246 LKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAGKHEIV 305 Query: 301 LTIDGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDEQGAHFAVQDTGIGISP 360 LTIDGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDEQGAHFAVQDTGIGISP Sbjct: 306 LTIDGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDEQGAHFAVQDTGIGISP 365 Query: 361 EHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTAHFPS 420 EHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFT HFPS Sbjct: 366 EHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTVHFPS 425 Query: 421 TQTIQDYAPTP 431 TQTIQDYA TP Sbjct: 426 TQTIQDYAQTP 436 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 115 bits (288), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 74/237 (31%), Positives = 125/237 (52%), Gaps = 23/237 (9%) Query: 195 ERIDMMRRDFVANASHELRTPLTVINGFLEI---ALAQPNLEEGTRMAHLKLMTEQGQRM 251 ER + +RR+F+A+ SHELRTPL ++NG LE + QP+ + L+ + + + + Sbjct: 242 ERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQT------LRSLKSEVEHL 295 Query: 252 QNLIDDMLTLTRLESIDYPLRTEVLRVRPLL--------ERIAEEGRALSAGKHEIVLTI 303 L+ D+ L+ ++ R + + PLL +R ++ G LS E+ ++ Sbjct: 296 NKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSC---ELPASM 352 Query: 304 DGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDEQGAHFAVQDTGIGISPEHI 363 + G+A L+ +NL N++RYT GGR+ + +Q H D+ G+ PE + Sbjct: 353 P---VHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSEPGVPPEVM 409 Query: 364 SRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTAHFPS 420 +RL ERF+RV+ SR+R + G GLGLAI R ++ H T+ ++ P G P+ Sbjct: 410 ARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPA 466 >gi|300310697|ref|YP_003774789.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 916 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 80/247 (32%), Positives = 124/247 (50%), Gaps = 20/247 (8%) Query: 194 SERIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLEEGTRMAHLKLMTEQGQRMQN 253 ++ ++ + F+ SH+LRTPLT I GF ++ A G H +++ + M Sbjct: 444 ADEANLAKSGFLTRISHDLRTPLTSILGFADMIQA----SGGPHAGHGRIIMRSARHMLT 499 Query: 254 LIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAGKHEIVLTIDGPDLKG--- 310 +++D++ R + +D P T L + LL+ IA+EG+ L+ +H PDL Sbjct: 500 MVNDLIDYARGDPLDTPQPTP-LYLHALLQEIAQEGQVLAQRQHNHFTLDISPDLPPVLA 558 Query: 311 -NAEELRSAFTNLVTNAVRYTPEGGRISL--RWQSDEQGA-----HFAVQDTGIGISPEH 362 +A L NL+ NA +YT EG RI L +WQ+D A V DTG GI+P+ Sbjct: 559 LDARRLHRILGNLLDNAAKYTREG-RIGLHVQWQADHADAGKGALQIEVSDTGCGIAPQF 617 Query: 363 ISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTAHFPSTQ 422 SR+ E F R D R + G G+GLAIVR + R ++ + S P G+ P+ + Sbjct: 618 QSRIFEPFERADADRRQP--GIGMGLAIVRLWVQRMGGSIVLESQPGMGTRMHLQLPAER 675 Query: 423 -TIQDYA 428 + QD A Sbjct: 676 GSEQDIA 682 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 83/246 (33%), Positives = 129/246 (52%), Gaps = 13/246 (5%) Query: 189 HDVTES--ERIDMMRRDF------VANASHELRTPLTVINGFLEIALAQP-NLEEGTRMA 239 H++ ES E +D + R F A+ +H++RTPL + ++AL +P ++E A Sbjct: 225 HEIVESFNEMLDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDE--YQA 282 Query: 240 HLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAGKHEI 299 L E+ +R+ +++ ML L R E+ L+ + L V L RIA+ + A + ++ Sbjct: 283 LLSSNIEEYERLSRMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGI-ADEAQV 341 Query: 300 VLTIDGPD-LKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDEQGAHFAVQDTGIGI 358 L + G L+ +A LR A NLV NA+RYTP G I L+ + G +V + G GI Sbjct: 342 RLVVQGAGRLQADATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGI 401 Query: 359 SPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTAHF 418 E + RL +RFYR D SRS +GLGLAIVR +++ H + S P + F+ F Sbjct: 402 PAEQLERLFDRFYRADPSRSSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRF 461 Query: 419 PSTQTI 424 P + Sbjct: 462 PGRSAV 467 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 83/230 (36%), Positives = 122/230 (53%), Gaps = 16/230 (6%) Query: 201 RRDFVANASHELRTPLTVINGFLEIALAQPNLEEGTRMAHLKLMTEQGQRMQNLIDDMLT 260 +R FVA+A+HELRTPLT + L+ LA+ ++E R A + +R +L+ +LT Sbjct: 216 QRAFVADAAHELRTPLTALR--LQTQLAERAVDEEERRAAFADLKNGLERTTHLVHQLLT 273 Query: 261 LTRLESIDYPLRTEVLRVRPLLERIAEEGRALSA-GKHEIVLTIDGPD-----LKGNAEE 314 L R E D T+V +R LL + + +++ G ++ L+ G D + GNA+ Sbjct: 274 LARQEP-DAMSFTQV-DLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADA 331 Query: 315 LRSAFTNLVTNAVRYTPEGGRISLRWQSDEQG-AHFAVQDTGIGISPEHISRLTERFYRV 373 LR F NLV NA+RYTP GG + + G VQD+G GI+ + R+ +RFYR Sbjct: 332 LRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFYRA 391 Query: 374 DKSR----SRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTFTAHFP 419 S+ SR G+GLGLAIVR + H +E+ + HG FP Sbjct: 392 QHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNT-GHGLQARVVFP 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,363,945 Number of extensions: 58060 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 40 Length of query: 431 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 343 Effective length of database: 1,203,190 Effective search space: 412694170 Effective search space used: 412694170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)