BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0260.p (541 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 1071 0.0 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 581 e-167 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 514 e-147 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 460 e-130 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 438 e-124 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 1071 bits (2769), Expect = 0.0, Method: Compositional matrix adjust. Identities = 540/541 (99%), Positives = 540/541 (99%) Query: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY Sbjct: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 Query: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG Sbjct: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 Query: 121 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA 180 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA Sbjct: 121 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA 180 Query: 181 HIQTVYRESRNLMLALAVLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDE 240 HIQTVYRESRNLMLALA LQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDE Sbjct: 181 HIQTVYRESRNLMLALAFLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDE 240 Query: 241 RLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAG 300 RLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAG Sbjct: 241 RLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAG 300 Query: 301 ALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRK 360 ALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRK Sbjct: 301 ALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRK 360 Query: 361 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKAL 420 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKAL Sbjct: 361 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKAL 420 Query: 421 IDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDM 480 IDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDM Sbjct: 421 IDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDM 480 Query: 481 DQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPAL 540 DQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPAL Sbjct: 481 DQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPAL 540 Query: 541 R 541 R Sbjct: 541 R 541 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 581 bits (1497), Expect = e-167, Method: Compositional matrix adjust. Identities = 329/540 (60%), Positives = 405/540 (75%), Gaps = 6/540 (1%) Query: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 M NM IGRRL LGFA+IL + ++ + +WRL +A AT EMM EPL ERL++DWY Sbjct: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 Query: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 + +RRT AIAKSSDP+L +FA + AA+S+ S E QK +EAL+ + +EKA+F K+G Sbjct: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 Query: 121 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA 180 E+RK Y+ RD I+KAKA G TEEA +IL F Y D +++L+ QR +D A Sbjct: 121 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR 180 Query: 181 HIQTVYRESRNLMLALAVLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDE 240 I + +SR L+L L L ++LG +FAR L LGIT+PL +AV+V+ VA GDL+ I Sbjct: 181 EIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEI-- 238 Query: 241 RLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAG 300 + +R DETG+L+ +L M +L IV VR GTD I+TAS EIA GNLDLS+RTE+QA Sbjct: 239 AVTSR-DETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQAS 297 Query: 301 ALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRK 360 +L ETASAMEEL STV QNADNARQA+QLA SAS+VA+QGG VVSQV+ TMG+IN SSRK Sbjct: 298 SLAETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRK 357 Query: 361 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKAL 420 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSASAAKEIK L Sbjct: 358 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKEL 417 Query: 421 IDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDM 480 I+DSV KV GS LV +AG+TM EVV+SVR+V DIV+EISAAS+EQ+ GI Q+NLAIT M Sbjct: 418 INDSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQM 477 Query: 481 DQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPAL 540 DQ TQQNAALVEEAAAAA +MQ QA RL ++VS F+L ++ + R +D+TP P L Sbjct: 478 DQVTQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLV---RREIDVTPSSPVL 534 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 514 bits (1324), Expect = e-147, Method: Compositional matrix adjust. Identities = 304/516 (58%), Positives = 364/516 (70%), Gaps = 11/516 (2%) Query: 6 NMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTIHT 65 N+KIG RLA+GF +L +V+VAL +WRL I ATD+M L KER W+ I Sbjct: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 Query: 66 SVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLGEVRKD 125 + RT A+ KS D + A+F A S ++ QK +EA I +EK +F ++G++R Sbjct: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAA 122 Query: 126 YIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAAHIQTV 185 Y R +I K G A ++ Y D + + Q+ IDQ AA I Sbjct: 123 YRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRD 182 Query: 186 YRESRNLMLALAVLQVVLG----WLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDER 241 YR R+++LAL +++VLG WL R+ ITRPL QAV +AE VAAGDLT RID Sbjct: 183 YRSGRSMLLALGAIEIVLGAALAWLLTRS----ITRPLNQAVTIAETVAAGDLTSRID-- 236 Query: 242 LAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAGA 301 ++ DETGKL+ AL M GNL IVG+VR GT+ I TAS+EIATGNLDLSARTE QAG+ Sbjct: 237 -SSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGS 295 Query: 302 LEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRKI 361 LEETASAME+LTSTV QNADNARQAN+LAASAS VA QGG VV QV++TMG+I+ SS+KI Sbjct: 296 LEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKI 355 Query: 362 VDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKALI 421 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIK LI Sbjct: 356 ADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLI 415 Query: 422 DDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDMD 481 DDSV KV GS LV RAG TM EVV SV +V +V EI+ AS EQSDGI QVNLAIT +D Sbjct: 416 DDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHID 475 Query: 482 QTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKL 517 +TTQQNAALVE+AAAAA +MQ+QAA L++ VS F+L Sbjct: 476 ETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRL 511 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 460 bits (1184), Expect = e-130, Method: Compositional matrix adjust. Identities = 274/512 (53%), Positives = 360/512 (70%), Gaps = 3/512 (0%) Query: 6 NMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTIHT 65 +++IG RL GFAV+L + VV A+ IWRL+ +A T +MM PL ERLVS W + Sbjct: 4 DIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTLLLI 63 Query: 66 SVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLGEVRKD 125 +++RTT + KS DP L AF A +AAA+S+ S + K +E L+ S++EK +F + RK Sbjct: 64 AIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVARKQ 123 Query: 126 YIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAAHIQTV 185 ++ RD I AK +G + ++ + K D ++ LL +RA IDQ++A IQ Sbjct: 124 FLTIRDQIYAAKKQGDAAQVEELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQIQQD 183 Query: 186 YRESRNLMLALAVLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDERLAAR 245 S++ ++ L V+ ++ G FA L ITRP+ A+ +++ VAAGDLT R A Sbjct: 184 ASSSQHQIMLLEVVILLCGIAFAVLLTRSITRPVHVALAISQRVAAGDLTSRAQ---AMG 240 Query: 246 GDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAGALEET 305 DE G+L+ +L M+ L +V VR G D+IA AS EIA GNLDLSARTE QAGALE+T Sbjct: 241 RDELGQLVASLQGMSERLHGVVSTVRHGADSIAMASGEIANGNLDLSARTEEQAGALEQT 300 Query: 306 ASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRKIVDII 365 A+++EELTS+V N+DNA QAN+L ++A+SVA QGG V+ Q+ISTM +IN+SSRKIVDII Sbjct: 301 AASIEELTSSVRTNSDNAHQANELVSTAASVAGQGGAVMDQLISTMATINDSSRKIVDII 360 Query: 366 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKALIDDSV 425 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIK LIDDSV Sbjct: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLIDDSV 420 Query: 426 AKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDMDQTTQ 485 KV +GS LV +AG TM EVV SV+ +V EIS AS +Q++GI QVN A+ MD TQ Sbjct: 421 GKVSAGSTLVEQAGKTMQEVVGSVQGAHTLVGEISTASRQQAEGIHQVNQAVGQMDGVTQ 480 Query: 486 QNAALVEEAAAAAEAMQEQAARLSEVVSQFKL 517 QNAALVE+AAAAA+++++QAA+L V+ F L Sbjct: 481 QNAALVEQAAAAAKSLEDQAAQLKRAVAFFSL 512 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 438 bits (1127), Expect = e-124, Method: Compositional matrix adjust. Identities = 277/527 (52%), Positives = 357/527 (67%), Gaps = 23/527 (4%) Query: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 M +F N+KI ++L + FA ++ ++ +I L+ + A+ +M ++W Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDM----------ATNWL 50 Query: 61 RTIHTSVRRTTAIAKSSDPSLAAFFA------DDA--AAASRMST--EQQKAIEALIESD 110 TI + ++ + L A +DA A SR+ +Q+ A EA I Sbjct: 51 PTIKAAQSIQASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEA 110 Query: 111 QEKAVFTKLGEVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTL 170 +EK ++ K + Y++ +++ AEG+ EEA + G + + ++ + +++ + Sbjct: 111 EEKVLYEKFSKSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKV 170 Query: 171 QRANIDQVAAHIQTVYRESRNLMLALAVLQVVLGWLFARTLALGITRPLEQAVEVAEAVA 230 A + + VYR + ++ L VV+ A +A ++RPL +AV VAE VA Sbjct: 171 NDAGSKRSDDMAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVA 230 Query: 231 AGDLTHRIDERLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLD 290 GDLT I A DETG LMH+L AM +L +IVG+VR GTD I TAS+EIATGNLD Sbjct: 231 QGDLTMDIR---ATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLD 287 Query: 291 LSARTERQAGALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVIST 350 LS+RTE QAG+LEETASAMEELT+TV QNADNARQANQLAASAS +A QGG VV QV+ T Sbjct: 288 LSSRTEEQAGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQT 347 Query: 351 MGSINESSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRS 410 M I ESS +I DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRS Sbjct: 348 MEGITESSNRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRS 407 Query: 411 ASAAKEIKALIDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGI 470 ASAAKEIK LIDDSV KVG+GS+LV RAG+TM EVVASVR+V D+V+EISAAS+EQS GI Sbjct: 408 ASAAKEIKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGI 467 Query: 471 SQVNLAITDMDQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKL 517 ++N AIT MD+ TQQNAALVEEAAAAA+++Q+QAARLS+VVS FKL Sbjct: 468 EEINRAITQMDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKL 514 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.125 0.325 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,065,586 Number of extensions: 32795 Number of successful extensions: 617 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 285 Number of HSP's successfully gapped: 71 Length of query: 541 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 451 Effective length of database: 1,193,720 Effective search space: 538367720 Effective search space used: 538367720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)