BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0259.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 510 e-146 gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein... 204 4e-54 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 129 2e-31 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 122 2e-29 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 118 4e-28 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 510 bits (1313), Expect = e-146, Method: Compositional matrix adjust. Identities = 249/250 (99%), Positives = 250/250 (100%) Query: 1 MNHYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVET 60 MNHYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVET Sbjct: 1 MNHYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVET 60 Query: 61 VKVDISSQADVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGA 120 VKVDISSQA+VQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGA Sbjct: 61 VKVDISSQAEVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGA 120 Query: 121 FYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNI 180 FYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNI Sbjct: 121 FYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNI 180 Query: 181 AVNAITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASV 240 AVNAITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASV Sbjct: 181 AVNAITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASV 240 Query: 241 FDLSGGRATY 250 FDLSGGRATY Sbjct: 241 FDLSGGRATY 250 >gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 204 bits (520), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 117/247 (47%), Positives = 152/247 (61%), Gaps = 10/247 (4%) Query: 4 YDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVETVKV 63 YDF A++TGG G G A++ RL G A+VV+WD A + R++ + Sbjct: 8 YDFGDCVAVVTGGHGGIGAAISARLAAGGAQVVVWDQHHDAASPYRQQ----------SI 57 Query: 64 DISSQADVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYV 123 DI+ V QA + T ID L+++AG AG P+ EY EW R++ ++L G F+ Sbjct: 58 DITDAPAVMQAAQELLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVFHA 117 Query: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIAVN 183 + V M A GRIVNIAS+AGKEG P ASAYSAAKAG++ALTKSLGKE A + VN Sbjct: 118 CRSVTPAMRAARRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAESGVLVN 177 Query: 184 AITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASVFDL 243 AI PAA +T +L Q + AH+ M++K P R V+E+A M AWL S SF T +VFDL Sbjct: 178 AIAPAAVKTALLGQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDL 237 Query: 244 SGGRATY 250 SGGRATY Sbjct: 238 SGGRATY 244 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 129 bits (324), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 79/243 (32%), Positives = 130/243 (53%), Gaps = 9/243 (3%) Query: 9 RSAIITGGAQGFGYAVAERLLQGKAKVVLW-----DMDEKALAEARRKLESLGNVETVKV 63 R A +TGG G G A+ RL + VV +K LA R E ++ + Sbjct: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMR---EQGFDIHASEG 60 Query: 64 DISSQADVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYV 123 ++S+ + A E +D+LV++AGI ++ + S ++W VID +LN F V Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGIT-RDGQFRKMSKDDWDAVIDTNLNSLFNV 119 Query: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIAVN 183 + V++ M + +GRI+NI+S+ G++G + YS AKAG+ T +L +E ATK + VN Sbjct: 120 TKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVN 179 Query: 184 AITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASVFDL 243 ++P T ++ ++ ++S IP R + EE+A++VAW+ S E + T S F L Sbjct: 180 TVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSL 239 Query: 244 SGG 246 +GG Sbjct: 240 NGG 242 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 122 bits (307), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 76/249 (30%), Positives = 133/249 (53%), Gaps = 5/249 (2%) Query: 1 MNHYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVET 60 M+ + QG+ A++TG ++G G A+A L + AKV+ E A L +LG E Sbjct: 1 MSAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGP-EA 59 Query: 61 VKVDISSQADVQQAIEATEKLTQ---SIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDL 117 + + + D + ++ E++ + S+ ILV++AGI Q+ EEW VI +L Sbjct: 60 GRGAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGIT-QDQLAMRMKDEEWDSVIATNL 118 Query: 118 NGAFYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETAT 177 +++ V++ M+ GRI+NI S+ G+ GNP Y+AAKAG+ ++++L +E + Sbjct: 119 TAVGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGS 178 Query: 178 KNIAVNAITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTT 237 +NI VN + P T + + +L +IP R EE+A VA+L S + ++ T Sbjct: 179 RNITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYIT 238 Query: 238 ASVFDLSGG 246 + +++GG Sbjct: 239 GTTLNVNGG 247 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 118 bits (296), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 71/249 (28%), Positives = 124/249 (49%), Gaps = 4/249 (1%) Query: 1 MNHYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGN-VE 59 + + +G+ A+ITG + G G A+A + Q A +VL D LA +L + G+ + Sbjct: 12 LQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIH 71 Query: 60 TVKVDISSQADVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNG 119 D +S +V+ A++ E I ILV++AGI + NP+ E+ +W +I +LN Sbjct: 72 ESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQ-RRNPLEEFKESDWHDLISTNLNS 130 Query: 120 AFYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKN 179 F+ Q V + MI + G+I+NI S+ + G P + Y+A K + TK + + Sbjct: 131 VFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYG 190 Query: 180 IAVNAITPAAAQTRI-LEQCTQAHI-DYMLSKIPRARFVKVEELAAMVAWLVSEENSFTT 237 I VN + P +T + + A D+++ + P R+ VE+L +L S+ + F Sbjct: 191 IQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVN 250 Query: 238 ASVFDLSGG 246 + + GG Sbjct: 251 GHILYVDGG 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 725,295 Number of extensions: 26888 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 46 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)