BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0258.p
         (281 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2...   573   e-165
gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578...   112   3e-26

>gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 281

 Score =  573 bits (1478), Expect = e-165,   Method: Compositional matrix adjust.
 Identities = 281/281 (100%), Positives = 281/281 (100%)

Query: 1   MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60
           MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN
Sbjct: 1   MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60

Query: 61  PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120
           PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK
Sbjct: 61  PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120

Query: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180
           TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP
Sbjct: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180

Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240
           IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS
Sbjct: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240

Query: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTIDA 281
           TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTIDA
Sbjct: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTIDA 281


>gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578
           [Herbaspirillum seropedicae SmR1]
          Length = 227

 Score =  112 bits (281), Expect = 3e-26,   Method: Compositional matrix adjust.
 Identities = 75/213 (35%), Positives = 108/213 (50%), Gaps = 13/213 (6%)

Query: 63  IGACVGNIGKFICIGLNYADHAAE-SNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKKT 121
           +G     + +  C+G NYA HA E  + P    P  F K   AVV  +  V  P  +   
Sbjct: 16  VGGAAFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSVVPYPVATSDY 75

Query: 122 DWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIER---GGTWDKGKGCDTF 178
             E+EL V + KGG  I  + A+  V GY V  D++ R+ Q +    G  WD  KG D  
Sbjct: 76  HHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWDMAKGFDQS 135

Query: 179 GPIGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDV 238
            P  P +V   +   P +  +WL+V+G+  Q G+ + +I++VA  +SYLS  + L PGD+
Sbjct: 136 APCAP-IVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETISYLSGLVELFPGDL 194

Query: 239 ISTGTPPGVGMGVKPEAVYLRAGQTIRLGIDGL 271
           I TGTP GVG  VK        G  ++  +DGL
Sbjct: 195 IFTGTPEGVGAVVK--------GDKLQGHVDGL 219


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.139    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,026,868
Number of extensions: 47490
Number of successful extensions: 117
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 116
Number of HSP's successfully gapped: 2
Length of query: 281
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 197
Effective length of database: 1,222,130
Effective search space: 240759610
Effective search space used: 240759610
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 130 (54.7 bits)