BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0251.p (431 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillu... 851 0.0 gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbasp... 92 1e-19 gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillu... 79 9e-16 >gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 851 bits (2199), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/431 (98%), Positives = 425/431 (98%) Query: 1 MSNYLNSTEAAARLGVSRQTLYAYVSRGLLHAEPGATARESRYFAADVERVAAQRAHGRK 60 MSNYLNSTEAAARLGVSRQTLYAYVSRGLLHAEPGATARESRYFAADVERVAAQRAHGRK Sbjct: 1 MSNYLNSTEAAARLGVSRQTLYAYVSRGLLHAEPGATARESRYFAADVERVAAQRAHGRK 60 Query: 61 PKEVAKAALNWGAPVLESSITLIDSGRLFYRGVDALSLAEHASLEEVAAGLWQCDRQTAF 120 PKEVAKAALNWGAPVLESSITLID GRLFYRGVDALSLAEHASLEEVAAGLWQCDR+TAF Sbjct: 61 PKEVAKAALNWGAPVLESSITLIDGGRLFYRGVDALSLAEHASLEEVAAGLWQCDRRTAF 120 Query: 121 GPHSAASPTVLRALFRHYGDQRAEEVLLPLFAVASDDASTAIWQKAVERQAQGCGDLVRI 180 GPH AASP VLRALFRHYGDQRAEEVLLPLFAVASDDASTAIWQKAVERQAQGCGDLVRI Sbjct: 121 GPHGAASPAVLRALFRHYGDQRAEEVLLPLFAVASDDASTAIWQKAVERQAQGCGDLVRI 180 Query: 181 LAACLLRTRPDTSPIHVQCARAWDVGRAGAELIRMALVLCADHELNASTFTSRCVASTDA 240 LAACLLRTRPDTSPIHVQCARAW VGRAG ELIRMALVLCADHELNAS+FTSRCVASTDA Sbjct: 181 LAACLLRTRPDTSPIHVQCARAWGVGRAGGELIRMALVLCADHELNASSFTSRCVASTDA 240 Query: 241 SLRAVVIAGLAGLSGGRHGATTGRVEALWDELGTRDVDRKMRERLSRGDDLPGFGHTLYP 300 SLRAVVIAGLAGLSGGRHGATTGRVEALWDELGTRDVDRKMRERLSRGDDLPGFGHTLYP Sbjct: 241 SLRAVVIAGLAGLSGGRHGATTGRVEALWDELGTRDVDRKMRERLSRGDDLPGFGHTLYP 300 Query: 301 DGDPRASYLLSQILPRHSPWAAMIDAGITLTGQKPSVDFALVALRRHLRLPVGAAFGLFA 360 DGDPRASYLLSQILPRHSPWAAMIDAGITLTGQKPSVDFALVALRRHLRLPVGAAFGLFA Sbjct: 301 DGDPRASYLLSQILPRHSPWAAMIDAGITLTGQKPSVDFALVALRRHLRLPVGAAFGLFA 360 Query: 361 LGRSIGWIAHGLEQREQHHLIRPRAVYIGPQPEDGQAEPGRPRSRARSANRQTQQGSVPK 420 LGRSIGWIAHGLEQREQHHLIRPRAVYIGPQPEDGQAEPGRPRSRARSA RQTQQGSVPK Sbjct: 361 LGRSIGWIAHGLEQREQHHLIRPRAVYIGPQPEDGQAEPGRPRSRARSAGRQTQQGSVPK 420 Query: 421 GDLPAGFFKTR 431 GDLPAGFFKTR Sbjct: 421 GDLPAGFFKTR 431 >gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbaspirillum seropedicae SmR1] Length = 384 Score = 91.7 bits (226), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 97/386 (25%), Positives = 161/386 (41%), Gaps = 58/386 (15%) Query: 51 VAAQRAHGRKPKE-VAKAALNWGAPVLESSITLIDSGR-LFYRGVDALSLAEHASLEEVA 108 ++ Q+A G KPK+ VA + + G L T+ +G L YRG D L +A+ EE+A Sbjct: 1 MSEQQAAGFKPKKSVALSGVTAGNTAL---CTVGKTGNDLHYRGYDILDVADSCEFEEIA 57 Query: 109 AGLWQCDRQTAFGPHSAASPTVLRALFRHYGDQRAEEVLLPLFAVASDDAST------AI 162 L TA A L++L + +A LP + D T + Sbjct: 58 HLLVHGKLPTA--AELRAYKAKLKSLRGLPANVKAALEWLPAASHPMDVMRTGVSALGCV 115 Query: 163 WQKAVERQAQGCGDLVRILAACL------------------LRTRPDTSPIHV------- 197 + + G D+ L A L + T D+ H Sbjct: 116 LPEKDDHNTPGARDIADRLMASLGSMLLYWYHYSHNGNRIEVETDDDSIGAHFLHLLHGE 175 Query: 198 QCARAWDVGRAGAELIRMALVLCADHELNASTFTSRCVASTDASLRAVVIAGLAGLSGGR 257 + + AW+ + + +L+L A+HE NASTFT R +A T + + + + + L G + Sbjct: 176 KPSEAWE------KAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPK 229 Query: 258 HGATTG---RVEALWDELGTRDVDRKMRERLSRGDDLPGFGHTLYPDGDPRASYL--LSQ 312 HG ++ +D + D +R R+ + + GFGH +Y DPR + +++ Sbjct: 230 HGGANEVAFEIQKRYDNPDEAEAD--IRRRVENKEVVIGFGHPVYTISDPRNKVIKEVAR 287 Query: 313 ILPRHSPWAAMIDAGITL-------TGQKPSVDFALVALRRHLRLPVGAAFGLFALGRSI 365 L + + M D L P++D+ + +P LF + R+ Sbjct: 288 KLSKEAGSTKMFDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTS 347 Query: 366 GWIAHGLEQREQHHLIRPRAVYIGPQ 391 GW AH +EQR + +IRP A Y+GP+ Sbjct: 348 GWAAHIIEQRIDNKIIRPSANYVGPE 373 >gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 78.6 bits (192), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 60/195 (30%), Positives = 95/195 (48%), Gaps = 23/195 (11%) Query: 217 LVLCADHELNASTFTSRCVASTDASLRAVVIAGLAGLSGGRHGATTGRVEALWDELGTRD 276 L+L ADHE NAST T R S+ A+ A + AG+A L G HG + +++G+ D Sbjct: 251 LILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVD 310 Query: 277 ----VDRKMRERLSRGDDLPGFGHTLYPDGDPRASYL-------LSQILPRHSPW----- 320 ++++++ S G L GFGH +Y + DPRA + L+++ P Sbjct: 311 KIPEFIKQVKDKNS-GVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAM 369 Query: 321 ----AAMIDAGITLTGQKPSVDFALVALRRHLRLPVGAAFGLFALGRSIGWIAHGLEQRE 376 A+ D P+VDF ++R L +P +FA+ R+IGWIA E Sbjct: 370 ELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEMIS 429 Query: 377 --QHHLIRPRAVYIG 389 + + RPR +++G Sbjct: 430 DPEQKIGRPRQLFVG 444 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,481,944 Number of extensions: 66149 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 237 Number of HSP's successfully gapped: 3 Length of query: 431 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 343 Effective length of database: 1,203,190 Effective search space: 412694170 Effective search space used: 412694170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)