BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0247.p
         (206 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate...   414   e-117
gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate...   107   6e-25
gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate...    66   2e-12

>gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 206

 Score =  414 bits (1064), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 201/206 (97%), Positives = 202/206 (98%)

Query: 1   MNGPAKLIEYWFDFGSNYSYLSTMRIEQAARQAGVRVVWRPFLLGPVFQELGWSTSPFVL 60
           MNGPAKLIEYWFDFGSNYSYLSTMRIEQAA+QAGVRVVWRPFLLGPVFQELGWSTSPFVL
Sbjct: 1   MNGPAKLIEYWFDFGSNYSYLSTMRIEQAAQQAGVRVVWRPFLLGPVFQELGWSTSPFVL 60

Query: 61  QKEKGAYTWKDMARQCRKYGLPWTMPSRFPRAAVLATRVALSFEHAPWMSSFCRLVMHLN 120
           QKEKGAYTWKDM RQCRKYGLPWTMPSRFPRAAVLATRVALSFE A WMSSFCRLVMHLN
Sbjct: 61  QKEKGAYTWKDMTRQCRKYGLPWTMPSRFPRAAVLATRVALSFEDALWMSSFCRLVMHLN 120

Query: 121 FAEDRDIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTRGIFGAPTFFV 180
           FAEDRDIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQART GIFGAPTFFV
Sbjct: 121 FAEDRDIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTLGIFGAPTFFV 180

Query: 181 GDEMFWGNDRLDDALAWCAGDAGVSR 206
           GDEMFWGNDRLDDALAWCAGDAGVSR
Sbjct: 181 GDEMFWGNDRLDDALAWCAGDAGVSR 206


>gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 200

 Score =  107 bits (267), Expect = 6e-25,   Method: Compositional matrix adjust.
 Identities = 62/186 (33%), Positives = 92/186 (49%), Gaps = 4/186 (2%)

Query: 8   IEYWFDFGSNYSYLSTMRIEQAARQAGVRVVWRPFLLGPVFQELGWSTSPFVLQKEKGAY 67
           I+++FDF S Y Y + + I++ A Q G  V W P LLGP+F+ LG  T+  V    KG Y
Sbjct: 5   IDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALG--TNSLVNIPVKGEY 62

Query: 68  TWKDMARQCRKYGLPWTMPSRFPRAAVLATRVALSFEHAPWMSS--FCRLVMHLNFAEDR 125
           +  DM R  R + + +  PS FP    +A R  L  +      +    + +    F ED 
Sbjct: 63  SRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQPAKAVELIKTLYSAYFTEDI 122

Query: 126 DIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTRGIFGAPTFFVGDEMF 185
           DI  V+ +  +   LG+    + A  +S   + +LR+ T  A   G+FG+P   V  E F
Sbjct: 123 DISVVDNVLRIAADLGIDRNALQAALESPELKDQLRQSTEAAGQAGVFGSPFMIVDGEQF 182

Query: 186 WGNDRL 191
           WG DR 
Sbjct: 183 WGFDRF 188


>gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 201

 Score = 65.9 bits (159), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 53/193 (27%), Positives = 92/193 (47%), Gaps = 7/193 (3%)

Query: 5   AKLIEYWFDFGSNYSYLSTMRIEQAARQAGVRVVWRPFLLGPVFQELGWSTSPFVLQK-E 63
           +K+ EY+F   S ++YL   R    A +  V+++ +PF L  +F   G    P   +  +
Sbjct: 2   SKVCEYFFAPHSPFAYLGHDRFVALADKYQVQILPKPFDLSKIFGVSG--GLPLAKRAPQ 59

Query: 64  KGAYTWKDMARQCRKYGLPWTM-PSRFPRAAVLATRVALSFEHAPWMSSFCRL---VMHL 119
           + AY  K++AR     G P  + P+ FP  +  A R+ ++ + A   ++  RL   VM  
Sbjct: 60  RQAYRLKELARWSEFLGKPMILQPAHFPVNSDNAARLIIATQLAHGTAAALRLAGAVMRA 119

Query: 120 NFAEDRDIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTRGIFGAPTFF 179
            + E ++I   +T+  +        A ++  A++   +A     T +A    +FGAP F 
Sbjct: 120 VWEEQKNIADPDTLVAIANVQEHDGAALLKSAETASVQAEFDRFTDEANAASVFGAPWFI 179

Query: 180 VGDEMFWGNDRLD 192
              E +WG DRLD
Sbjct: 180 YEGEGYWGQDRLD 192


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.136    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 660,057
Number of extensions: 25396
Number of successful extensions: 114
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 111
Number of HSP's successfully gapped: 3
Length of query: 206
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 125
Effective length of database: 1,236,335
Effective search space: 154541875
Effective search space used: 154541875
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 128 (53.9 bits)