BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0244.p (703 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitr... 1264 0.0 gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 78 3e-15 gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 70 6e-13 gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 62 1e-10 >gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitrate transport protein [Herbaspirillum seropedicae SmR1] Length = 703 Score = 1264 bits (3272), Expect = 0.0, Method: Compositional matrix adjust. Identities = 613/703 (87%), Positives = 649/703 (92%) Query: 1 MMTPQSPSHFTRDLRITTTTLASLVMLAFPLAGLAQQTTTPPGQAALEPIQVSGDWLGTG 60 M T Q+PS F RDLRITTT LASL +LAFP+AGLAQQ + GQ LEPIQVSGDWLGTG Sbjct: 1 MKTHQAPSLFVRDLRITTTALASLAILAFPVAGLAQQASPSDGQPGLEPIQVSGDWLGTG 60 Query: 61 LESSVKTYPGARTVVKKQEIDSTGAVNIGDVMRRIPGVQSSESAGSAGSAVVLNIGVRGL 120 LESSVKTYPGARTVVKKQEI+STGAV+IGD MRRIPGVQSS+++G+AGSA+ LNIGVRGL Sbjct: 61 LESSVKTYPGARTVVKKQEIESTGAVSIGDAMRRIPGVQSSDNSGTAGSAIALNIGVRGL 120 Query: 121 AGRYSPRSTILLDGIPLAVAPYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVGGII 180 AGRYSPRSTILLDGIP+AVAPYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVGGII Sbjct: 121 AGRYSPRSTILLDGIPMAVAPYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVGGII 180 Query: 181 NFKTRSIPATIGISGDASVRYNSYGKGGGNAQYTAFAGGQAENGFGMAFLYSGKDGSGWR 240 NFKTRSIP T GISGDA+VRYNSYG+GGGN QYTAFAGGQAENGFGMAFLYSG++GSGWR Sbjct: 181 NFKTRSIPTTPGISGDAAVRYNSYGQGGGNTQYTAFAGGQAENGFGMAFLYSGQEGSGWR 240 Query: 241 DKSDEHLNDFALKMRYEFSPTSELYAKINYYDVLSRTPGGLSVAQYAADPFQNTRQRDNW 300 DKSDEHLNDFALKMRYE SPTSELYAKINYYDVLSRTPGGLSVAQYAADPFQ+TRQRDNW Sbjct: 241 DKSDEHLNDFALKMRYEISPTSELYAKINYYDVLSRTPGGLSVAQYAADPFQSTRQRDNW 300 Query: 301 SGTRKGFDVGYLNTISDRQELEIRTYFSQATRQSTLIRAAGTTLSHNPRAYETLGIEPRY 360 GTRKGFDVGYLNTISDRQE EIRTYFSQATRQSTLI AGT LSH PR YETLGIEPRY Sbjct: 301 GGTRKGFDVGYLNTISDRQEFEIRTYFSQATRQSTLINPAGTRLSHQPRNYETLGIEPRY 360 Query: 361 THRFAWGGSTHDVTVGYRYLRERGEDSSYEEVVRTGVLSNYAPFQNSTDAHAGYIDDKIA 420 T RFAWGG+THDVTVGYRY+RERG+D+ Y E V TGVLS + FQNSTDAHAGYIDDKIA Sbjct: 361 TQRFAWGGTTHDVTVGYRYVRERGDDNVYSEAVLTGVLSGFTQFQNSTDAHAGYIDDKIA 420 Query: 421 LGKWRITPGLRFEHVQSTRYEVNKKASYEVTNNQPLPSINVAYLLNEQLTVFSNYNTSFG 480 GKWRITPGLRFEHVQSTRY+V ASY+V+NN+PLPSINVAYLL EQLT+FSNYNTSFG Sbjct: 421 FGKWRITPGLRFEHVQSTRYDVANNASYQVSNNKPLPSINVAYLLTEQLTLFSNYNTSFG 480 Query: 481 VVQNSQLNNMSPTSPLSPELAKTIEGGLRWKSAQALAEATVFHIDFDNQIESIQGTNPSV 540 VVQNSQLN MS +PLSPELAKTIEGG+RWKSA+A AEATVFHIDFDNQI S+QGTNPSV Sbjct: 481 VVQNSQLNTMSSANPLSPELAKTIEGGVRWKSARASAEATVFHIDFDNQIVSVQGTNPSV 540 Query: 541 SRNVGSTQHEGIELAIDYAFDQQSVLKGWSVYANYTYTRALLKSGKNVGKDLPFYARTTD 600 RN+G TQH+GIELA+DYAFDQQSVLKGWSVYANYTYTRAL KSG N GKDLPFYARTTD Sbjct: 541 FRNIGKTQHDGIELAMDYAFDQQSVLKGWSVYANYTYTRALQKSGANPGKDLPFYARTTD 600 Query: 601 TVGTRFKSGPWGVNLSTTHQSRQFSDEANTIAESADGSIGEIPGFRLWNAQVEWKSPGKK 660 TVGTRFKSGPWG NLSTTHQ RQFSDEANTIAESADGSIGEIPGFRLWNAQV+WK PG K Sbjct: 601 TVGTRFKSGPWGFNLSTTHQGRQFSDEANTIAESADGSIGEIPGFRLWNAQVDWKVPGTK 660 Query: 661 GFDVFAGINNLTDRRYFTRTTDPNRGKLVGAPRTVYVQGRYAF 703 GFDVFAGINNLTDRRYFTRTTD N GKLVGAPRTVYVQGRY+F Sbjct: 661 GFDVFAGINNLTDRRYFTRTTDNNLGKLVGAPRTVYVQGRYSF 703 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 77.8 bits (190), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 167/751 (22%), Positives = 279/751 (37%), Gaps = 119/751 (15%) Query: 14 LRITTTTLASLVMLAFPLAGLAQQTTTPPGQAALEPIQVSGDWL-----------GTGLE 62 LR+ SL LA L AQQ TT AL I+VSG GT E Sbjct: 8 LRLAVLATCSLSPLAI-LPACAQQDTT-----ALPAIEVSGARANDGYVAPTAVSGTKSE 61 Query: 63 SSVKTYPGARTVVKKQEIDSTGAVNIGDVMRRIPGVQSSESAGSAGSAVVLNIGVRGLAG 122 + ++ P VV Q I A ++ DV++ +PG+ S G + +RG Sbjct: 62 APLRDVPQTINVVPAQVIRDQNASSLQDVLKNVPGLGLSTGDGQRD-----QVFIRG--- 113 Query: 123 RYSPRSTILLDGIPLAVAPYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVGGIINF 182 ++ +DG + L F LS N+ES++V++G AV +G + GG+IN Sbjct: 114 -FTAIGDQFVDG-------FRDDALYFRDLS--NVESVEVIKGPAAVLYGRGSSGGLINR 163 Query: 183 KTRSIPATIGISGDASVRYNSYGKGGGNAQYTAFAGGQAENGFGMAFLYSGKDGSGWRDK 242 T+ + + V + + G A+ G E + + G +R + Sbjct: 164 VTKKPGVDV-----SDVSFTANSTAGRRAELD--LGRAGEGALSWRLTGAVERGDSFRAQ 216 Query: 243 SDEHLNDFALKMRYEFSPTSELYAKINYYDVLSRTPGGLSVAQYAADPF----------Q 292 A + FS ++L +++Y + R + Y P + Sbjct: 217 QFIDRTAIAPSVALRFSADTKLLLQMDYLE--DRRMMDFGIPSYRGRPVDVDPGTTYGAR 274 Query: 293 NTRQRD----NWSGTRKGFDVGYLNTISDRQELEIRTYF--SQATRQSTLIRA-AGTTLS 345 N R D + T F + + S R L Y T T+ A A TTL+ Sbjct: 275 NARDADFVQSRVASTTATFTHRFNDEFSFRNGLRYYDYHLNRNNTNGGTVNEATATTTLN 334 Query: 346 HNPRAYETLGI--EPRYTHRFAWGGSTHDVTVGYRYLRERGEDSSYEEVVRTGVLSNYAP 403 H + G + T + G H++ G + + S VV +S + P Sbjct: 335 HAKLVRDENGWFNQSELTQKLQLGSIRHEILYGLELGSQSKDASQASGVVNFPGVSIFNP 394 Query: 404 FQ---------------NSTDAHAGYIDDKIALG-KWRITPGLRFEHVQSTRYEVN---- 443 + D A Y+ D ++L +W+ GLR++ + +N Sbjct: 395 VLPVVDPNRLNPPTLTYGTYDTFATYLQDSLSLSEQWKALVGLRYDRYRQQSRILNLANG 454 Query: 444 -----KKASYEVTNNQPL---PSINVAYLLNEQLTVFSNYNTSFGVVQNSQLNNMSPTSP 495 ++ V+ L PS+ +Y L+ + F SF + N+ + Sbjct: 455 STADLQRTDTAVSPRAGLVWQPSLTQSYYLSWSRS-FQPSGESFALATNN--------AD 505 Query: 496 LSPELAKTIEGGLRWKSAQALAEAT--VFHIDFDNQIESIQGTNPS-VSRNVGSTQHEGI 552 L+PE + E G ++ + A AT VF ++ DN I+ P+ V VG + +G Sbjct: 506 LAPEQTRNTEAGAKYDFWEGRASATVSVFRLERDN----IKSALPNGVVIPVGKQRTDGA 561 Query: 553 ELAIDYAFDQQSVLKGWSVYANYTYTRALLKSGKNVGKDLPFYARTTDTVGTRFKSGPWG 612 EL+ Q GW + A Y + A++ + +PF + T+ R W Sbjct: 562 ELSFSADLKQ-----GWKLLAGYAWLNAVITESSSFDNGVPFKGKNA-TLTPRHSGNLWL 615 Query: 613 VNLSTTHQSRQFSDEANTIAESADGSIGEIPGFRLWNAQVEWKSPGKKGFDVFAGINNLT 672 A + G+ +PG+ +A +++ ++V I NL Sbjct: 616 TRELGEGLVVGGGANAVGARFANPGNTVTLPGYVTVDAMAAYRT---SKYEVQLNIGNLF 672 Query: 673 DRRYFT--RTTDPNRGKLVGAPRTVYVQGRY 701 + Y T+PN + GAPR V + RY Sbjct: 673 NTGYIISGHGTNPNL-SMPGAPRNVALTLRY 702 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 70.1 bits (170), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 123/562 (21%), Positives = 223/562 (39%), Gaps = 76/562 (13%) Query: 58 GTGLESSVKTYPGARTVVKKQEIDSTGAVNIGDVMRRIPGVQSSESAGSAGSAVVLNIGV 117 T ++ V P + +VV + E+D+ G ++ DV+R +PG S N+ Sbjct: 58 ATKTDTPVLEVPQSVSVVTRDEMDARGVTSLLDVLRYMPGANSETHGVDPRGYEYFNL-- 115 Query: 118 RGLAGRYSPRSTILLDGIPLAVAPYGQPQLSFAPLSLNNIESIDVVRGGGAVRFGPQNVG 177 RG + ++ L+G+ +GQ + +E I+V+RG G+V FG + G Sbjct: 116 RGFIN--AQVTSNFLNGLRQIPGGFGQFRTE-----TYGMERIEVLRGPGSVTFGQADPG 168 Query: 178 GIINFKTR----SIPATIGISGDASVRYNSYGKGGGNAQYTAFAGGQAENGFGMAFLYSG 233 G+IN ++ IP +G+ + R G+ Q + G Y Sbjct: 169 GVINRVSKLPGMDIPNELGVDIGSFNRKQVVADLNGHTQDGEYLWRVVGLGLDSNNQYKF 228 Query: 234 KDGSGWRDKSDEHLNDFALKMRYEFSPTSELYAKINYYDVLSRT-------PGGLSVAQY 286 +G D + A + + S ++ L +Y + S + PG + Sbjct: 229 NNGQ----SGDNNRFYLAPSLTWRPSASTSLTLMADYMNDRSGSSRWTAVRPGNVLTHTL 284 Query: 287 AADPFQNTRQRDNWSGTRKGFDVGYLNTISDRQELEIRTYFSQATRQSTL---------- 336 DP + + D WS + F+ + N + RQ F QA +S Sbjct: 285 IGDPNFDRQSNDQWSFGYQ-FEHKFDNAWTFRQN------FRQANLKSVYAALNPGTLTG 337 Query: 337 -IRAAGTTLSHNPRAYETLGIEPRYTHRFAWGGSTHDVTVGY-------RYLRERGE--- 385 + GTT+ +N + TL ++ + R WG + H V G R +R RG Sbjct: 338 SVLTRGTTV-YNSQVDNTL-VDNQLEGRLKWGATEHTVLFGLDWSRMYDREVRYRGAAPS 395 Query: 386 ---DSSYEEVVRTGVLSNYAPFQNSTDAHAGYIDDKIALG-KWRITPGLRFEHVQSTRYE 441 D+ V + + + + + Y D+I + +T G R++ V+ Sbjct: 396 LNIDNPVYGVTIPAATTLFGNLEENMNQTGLYAQDQIKFDQRLTLTLGGRWDKVKDETRN 455 Query: 442 VNKKASYEVTNNQPLPSINVAYLLNEQLTVFSNYNTSFGVVQNSQLNNMSPTSPLSPELA 501 + + ++ I ++YL+ + + +Y TSF S N P P A Sbjct: 456 YFNRTTTRSDDSALSGRIGLSYLVTRDIAPYVSYATSFLPQTGSDFNG----KPFDPTKA 511 Query: 502 KTIEGGLRWKSA--QALAEATVFHIDFDNQIESIQGTNPSVSR-NV--GSTQHEGIELAI 556 + E G++++ A + L A +F + N +Q +P+ + NV G + G+EL Sbjct: 512 RQAEVGIKYQPADGKTLFTAALFELTKTN----VQTPDPAHTNFNVLSGEIKSRGLELEA 567 Query: 557 DYAFDQQSVLKGWSVYANYTYT 578 + ++KG ++ A+YTYT Sbjct: 568 -----KGEIVKGLNLSASYTYT 584 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 62.4 bits (150), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 62/274 (22%), Positives = 116/274 (42%), Gaps = 29/274 (10%) Query: 414 YIDDKIALG-KWRITPGLRFEHVQSTRYEVNKKASYEVTNNQPLPSINVAYLLNEQLTVF 472 Y+ D+++L +WR+ G+R + +ST + A + P + + YLL +V+ Sbjct: 428 YLQDQLSLSERWRLLAGVRADAFRSTLNNLRNGAVTRQDKHAYSPRLGLTYLLTPSWSVY 487 Query: 473 SNYNTSFGVVQNSQLNNMSPTSPLSPELAKTIEGGLRWKSAQALAEATVFHIDFDNQ-IE 531 + + SF S ++ + SPE ++ E GL+++S + AT+ D Q + Sbjct: 488 VSASKSFRPNSGSDVSG----NAFSPETSRAAEAGLKFQSEDKRSGATLAVFDITKQNVL 543 Query: 532 SIQGTNPSVSRNVGSTQHEGIELAIDYAFDQQSVLKGWSVYANYTYTRALLKSGKN--VG 589 + NP+ S G + +G+EL + W V N YT A + VG Sbjct: 544 TTDPANPNFSIAAGEVRSKGVELDANARLGHH-----WRVTGNLAYTDAYVSRDNTLPVG 598 Query: 590 KDLPFYARTTDTVGTRFKS-----GPWGVNLSTTHQSRQFSDEANTIAESADGSIGEIPG 644 L R++ ++ F+ P+GV + R+ D+A + +P Sbjct: 599 SRLVNIPRSSASLLAVFEDADAHGAPYGVGGGLNYVGRRTGDQAGSFM---------LPS 649 Query: 645 FRLWNAQVEWKSPGKKGFDVFAGINNLTDRRYFT 678 + A W+ K + +NNL ++RY++ Sbjct: 650 YVTARALAYWQFTPKMKLSL--DVNNLFNKRYYS 681 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,264,355 Number of extensions: 97725 Number of successful extensions: 295 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 10 Length of query: 703 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 611 Effective length of database: 1,184,250 Effective search space: 723576750 Effective search space used: 723576750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 134 (56.2 bits)