BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0240.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis tra... 593 e-171 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 98 1e-21 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 96 6e-21 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 89 5e-19 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 89 7e-19 >gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 593 bits (1530), Expect = e-171, Method: Compositional matrix adjust. Identities = 295/302 (97%), Positives = 296/302 (98%), Gaps = 1/302 (0%) Query: 1 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQAT 60 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQAT Sbjct: 1 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQAT 60 Query: 61 LDLSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEAEQRFVTPEGKALVAKAR 120 LDLSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEAEQRFVTPEGKALVAKAR Sbjct: 61 LDLSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEAEQRFVTPEGKALVAKAR 120 Query: 121 EAFTKYKNAVMLTAEELRKEPLADARQSTSRLFSSVRPLANEAEANMLKLMEVKKDNADR 180 EAFTKYKNAVM TAEELRKEPLADARQSTSRLFSSVRPLANEAEANMLKLMEVKKDNADR Sbjct: 121 EAFTKYKNAVMQTAEELRKEPLADARQSTSRLFSSVRPLANEAEANMLKLMEVKKDNADR 180 Query: 181 LNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAAAADAIASGD 240 LNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAAAADAIASGD Sbjct: 181 LNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAAAADAIASGD 240 Query: 241 LSTHIDISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNLSVGAHRTT 300 LSTHIDISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNL + A RT Sbjct: 241 LSTHIDISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNLDLSA-RTE 299 Query: 301 GQ 302 Q Sbjct: 300 QQ 301 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 97.8 bits (242), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 90/312 (28%), Positives = 154/312 (49%), Gaps = 27/312 (8%) Query: 1 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYE--LQVVGLRHSAQ 58 M WF NLK++ KLI F V ++ +G F I + +N +T+M L + S Q Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 Query: 59 ATLD-LSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEA--EQRFVTPEGKAL 115 A+L + S + TA +PAERE +L + RLD LA A E + E K L Sbjct: 61 ASLPRMRISELQMVTA---ATPAEREDALKAIKSRLD--ILAKQRAAYEAQISEAEEKVL 115 Query: 116 VAKAREAFTKY----KNAVMLTAEELRKEPLADARQSTSRLFSSVRPLANEAEANMLKLM 171 K ++F Y K ++AE +E A + +++ F S+ NM +++ Sbjct: 116 YEKFSKSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIE-------NMDEII 168 Query: 172 EVKKDNADRLNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAA 231 +V + R +D + +Y ++ L V+ + L + ++R ++R L + A Sbjct: 169 KVNDAGSKRSDDMAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLN----EAVA 224 Query: 232 AADAIASGDLSTHIDISHAAQ-GSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATG 290 A+ +A GDL+ I + + G ++ ++ M SL+++V VRT +++I T S++IATG Sbjct: 225 VAERVAQGDLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATG 284 Query: 291 NLSVGAHRTTGQ 302 NL + + RT Q Sbjct: 285 NLDL-SSRTEEQ 295 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 95.5 bits (236), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 85/315 (26%), Positives = 151/315 (47%), Gaps = 33/315 (10%) Query: 1 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQAT 60 M WF +L++S KLI F++V + A +G F I + Q+N +TE+ + +R A+ Sbjct: 1 MKWFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQ 60 Query: 61 LDLSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLA-------LNEAEQRFVTPEGK 113 L +S ILA+ E E + +++N A ++E E++ + PE + Sbjct: 61 LAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVE 120 Query: 114 ALVAKAREAFTKYKNAVMLTAEELRKEPLADAR-----QSTSRLFSSVRPLANEAEANML 168 L+A F + ++ + + + E +AR +ST +S++ + Sbjct: 121 KLIA----TFVSEHDKIIALSRQGKDE---EARNLQRGESTKAYRTSLQQID-------- 165 Query: 169 KLMEVKKDNADRLNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRD 228 KL V AD N + D IYA R ++V + + L +++SR + R L + Sbjct: 166 KLAAVNDKGADISNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPL----EE 221 Query: 229 VAAAADAIASGDLSTHIDISHAAQ-GSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQI 287 + A +A GDL+T I+ + + G ++ A+ M SL +V VR +++I T S +I Sbjct: 222 AVSVAQRVAGGDLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEI 281 Query: 288 ATGNLSVGAHRTTGQ 302 A+GNL + + RT Q Sbjct: 282 ASGNLDL-SSRTEEQ 295 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 89.0 bits (219), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 76/306 (24%), Positives = 150/306 (49%), Gaps = 10/306 (3%) Query: 1 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQAT 60 M +++K+S +L F + ++ I G+ ++D E+ + V + Q T Sbjct: 1 MMKISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQIT 60 Query: 61 LDLSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEAEQRFVTPEGKALVAKAR 120 +++ R++R AI+A +P E++ L+ + + L E E+R TP G L+A + Sbjct: 61 DKVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALK 120 Query: 121 EAFTKYKNAVMLTAEELRKEPLADARQSTSRLFSSVRPLANEAEANMLKLMEVKKDNADR 180 +A Y + + + ++ +A T+ LF P+ ++ A + +++ + D+ Sbjct: 121 QARADYSKERIKVVDLIHQQKRDEA---TAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDK 177 Query: 181 LNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAAAADAIASGD 240 + + ++LVL+ VL + I+R++TR L + A A+A+GD Sbjct: 178 SAERASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLN----EAVQVATAVAAGD 233 Query: 241 LSTHID-ISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNLSVGAHRT 299 L+ I+ S G+++ A+ M +L ++V VR +++I T SR+IATGNL + + Sbjct: 234 LTVQIEATSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSR-- 291 Query: 300 TGQRPG 305 T Q+ G Sbjct: 292 TEQQAG 297 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 88.6 bits (218), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 78/307 (25%), Positives = 140/307 (45%), Gaps = 12/307 (3%) Query: 1 MNWFTNLKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQAT 60 MNWF NLK++HKL F++V + +G F ++ +N +T++ + + ++ + Sbjct: 1 MNWFYNLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMS 60 Query: 61 LDLSSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEAEQRFVTPEGKALVAKAR 120 L L+ S +L E +AS K++ + + P K L + Sbjct: 61 LALARSRSFDMQQLLTADKQEEKASRERADKQMATLLKEVARYGKLVSEPREKELYPEIE 120 Query: 121 EAFTKYKNAVMLTAEELRKEPLADARQSTSRLFSSVR-PLANEAEANMLKLMEVKKDNAD 179 YK A E + D +Q+ L +S P+ + + + +L EV A Sbjct: 121 RNIALYKEA----HERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGAA 176 Query: 180 RLNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAAAADAIASG 239 + + D IY +L L V+ +AL +I+R + R L + A +A+G Sbjct: 177 LSDADADHIYGQANMAILALVTTCVVLAMALSWWIARLVARPLA----NAVDIARQVAAG 232 Query: 240 DLSTHIDISHAAQ-GSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNLSVGAHR 298 DL+ I +H + G ++ A+ M G+L +V+ VR +++I T S +IATGN+ + + Sbjct: 233 DLTADIPTAHRDETGQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGNMDLSSR- 291 Query: 299 TTGQRPG 305 T Q+ G Sbjct: 292 -TEQQAG 297 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 825,618 Number of extensions: 28975 Number of successful extensions: 261 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 45 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)