BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0235.p (99 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313965|ref|YP_003778057.1| hypothetical protein Hsero_4683... 61 2e-11 >gi|300313965|ref|YP_003778057.1| hypothetical protein Hsero_4683 [Herbaspirillum seropedicae SmR1] Length = 165 Score = 61.2 bits (147), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 27/75 (36%), Positives = 47/75 (62%) Query: 24 LMQRFSLKTKRALFKDMHKCHLSAAQDVLKIEPQRHAKLEGWARERDDGIEDVIIAANSF 83 +MQR K ++A+ + M C ++A + ++I+P H ++G++ +DG E + I+ NS Sbjct: 86 IMQRHGYKNRKAMMRKMACCWITADKAKIEIKPTHHKNIDGYSGISNDGPEIIFISTNSG 145 Query: 84 PEEIGAALRLAFSRC 98 E+GAAL+ FSRC Sbjct: 146 DAELGAALKEGFSRC 160 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 287,477 Number of extensions: 9176 Number of successful extensions: 24 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 24 Number of HSP's successfully gapped: 1 Length of query: 99 Length of database: 1,619,870 Length adjustment: 68 Effective length of query: 31 Effective length of database: 1,297,890 Effective search space: 40234590 Effective search space used: 40234590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)