BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0229.p (568 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 1142 0.0 gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis tra... 381 e-107 gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sen... 373 e-104 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 357 2e-99 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 354 1e-98 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 1142 bits (2955), Expect = 0.0, Method: Compositional matrix adjust. Identities = 564/568 (99%), Positives = 565/568 (99%) Query: 1 MKISLVSRFSIRQIFIMLTAVITVLMTLIVLAALAVDRYDARLNAANESRYASFLLATEL 60 MKISLVSRFSIRQIFIMLTAVITVLMTLIVLAALAVDRYDARLNAANESRYASFLLATEL Sbjct: 1 MKISLVSRFSIRQIFIMLTAVITVLMTLIVLAALAVDRYDARLNAANESRYASFLLATEL 60 Query: 61 RQSSDDLTRLARTYVVTGDPSYEQQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRP 120 RQSSDDLTRLARTYVVTGDPSYEQQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRP Sbjct: 61 RQSSDDLTRLARTYVVTGDPSYEQQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRP 120 Query: 121 DSNQHVALTDLMRAAGFTAAEMGKLEEAKNNSDALVKTEVIAMNMVKGLYADAQGNFTKR 180 DSNQHVALTDLMRAAGFTAAEMGKLEEAKNNSDALVKTEVIAMNMVKGLYADAQGNFTKR Sbjct: 121 DSNQHVALTDLMRAAGFTAAEMGKLEEAKNNSDALVKTEVIAMNMVKGLYADAQGNFTKR 180 Query: 181 ADPDLAQARELMHNHAYHVYKAKIMRPVDEFFALLDARTAEQVESARAQATQMATLFYSL 240 ADPDLAQARELMHNHAYHVYKAKIMRPVDEFFALLDART EQVESARAQAT MATLFYSL Sbjct: 181 ADPDLAQARELMHNHAYHVYKAKIMRPVDEFFALLDARTTEQVESARAQATHMATLFYSL 240 Query: 241 IAIALVVLACTLMLVYHAIRNPLAHAASVARRVAHGDLTAQIDPEIKGETGELLEALTIM 300 IAIALVVLACTLMLVYHAIRNPLAHAASVARRVAHGDLTAQIDP IKGETGELLEALTIM Sbjct: 241 IAIALVVLACTLMLVYHAIRNPLAHAASVARRVAHGDLTAQIDPAIKGETGELLEALTIM 300 Query: 301 QSGLQTIVLDVRNGAQYINTSANEIASGTLDLSSRSEQQASSLEETASSMEELTSTVKQN 360 QSGLQTIVLDVRNGAQYINTSANEIASGTLDLSSR+EQQASSLEETASSMEELTSTVKQN Sbjct: 301 QSGLQTIVLDVRNGAQYINTSANEIASGTLDLSSRTEQQASSLEETASSMEELTSTVKQN 360 Query: 361 AGNARLASELANTASGVAVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILA 420 AGNARLASELANTASGVAVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILA Sbjct: 361 AGNARLASELANTASGVAVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILA 420 Query: 421 LNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAG 480 LNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAG Sbjct: 421 LNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAG 480 Query: 481 STMREVVESVQRVTEIITGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASE 540 STMREVVESVQRVTEIITGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASE Sbjct: 481 STMREVVESVQRVTEIITGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASE 540 Query: 541 SMQMQAHKLTDLVGAFKTPTAMGMMVVA 568 SMQMQAHKLTDLVGAFKTPTAMGMMVVA Sbjct: 541 SMQMQAHKLTDLVGAFKTPTAMGMMVVA 568 >gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 381 bits (979), Expect = e-107, Method: Compositional matrix adjust. Identities = 227/561 (40%), Positives = 339/561 (60%), Gaps = 20/561 (3%) Query: 1 MKISLVSRFSIRQIFIMLTAVITVLMTLIVLAALAVDRYDARLNAANESRYASFLLATEL 60 M+ ++ R SI+Q+FI L V+++L+ ++V A+ + L A++SRY S LA EL Sbjct: 1 MRETMTHRMSIKQLFIWLALVLSILVAVVVSLTGALKDANLELERAHQSRYDSTALANEL 60 Query: 61 RQSSDDLTRLARTYVVTGDPSYEQQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRP 120 R++++D+T+ AR YV TGDP+ E +Y I+DIRNGK+ARP +Y R WD V A A P Sbjct: 61 RRTAEDMTKFARAYVTTGDPNDEDRYYMIMDIRNGKRARPSNYDRFNWDLVNARKLALDP 120 Query: 121 DSNQHVALTDLMRAAGFTAAEMGKLEEAKNNSDALVKTEVIAMNMVKGLYADAQGNFTKR 180 + V L +LM+ AGFT E+ K++EA D L K E+ A + VKG + D +G +T Sbjct: 121 GA-PGVPLGELMKRAGFTDEELAKMQEALKLGDQLAKIEITAFHAVKGEFDDGEGKYTVI 179 Query: 181 ADPDLAQARELMHNHAYHVYKAKIMRPVDEFFALLDARTAEQVESARAQATQMATLFYSL 240 P+ A A+EL+ + Y K+M PV++F L+D RT V A ++ + L Sbjct: 180 GAPNQAMAQELVFDQTYRTLFGKLMSPVNDFLRLVDERTQANVRQAGKDYAELEQKIFWL 239 Query: 241 IAIALVVLACTLMLVYHAIRNPLA----HAASVARRVAHGDLTAQIDPEIKGETGELLEA 296 +A ++VVL L L Y IR+ + A SV RRVA GDLT + P KG+ +L + Sbjct: 240 LAASVVVLFACLWLSYRIIRSQIGGEPRDAMSVLRRVAEGDLTVVV-PLPKGDKLSVLYS 298 Query: 297 LTIMQSGLQTIVLDVRNGAQYINTSANEIASGTLDLSSRSEQQASSLEETASSMEELTST 356 M + ++ DV A + +++ +I+S + LS + QQA+++EET++S+EE+T+T Sbjct: 299 TQQMINKWTDVIGDVSGTATSLASASEQISSSSQALSHNAAQQAANVEETSASVEEITAT 358 Query: 357 VKQNAGNARLASELANTASGVAVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQT 416 + QNA NAR +A+ ++ A GGE V V M K+I I +ID IA+QT Sbjct: 359 IAQNADNARTTDGIASLSASAATEGGEAVRETVAAM-------KQIASKIGIIDDIAYQT 411 Query: 417 NILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLV 476 N+LALNAA+EAARAGE G+GFAVVA EVR LA+RS +AA+EI + + SV L Sbjct: 412 NLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQSAAQEIIAVAETSVG-------LA 464 Query: 477 NEAGSTMREVVESVQRVTEIITGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQAS 536 +AG + ++V S+++ +++ I+ AS EQ++G+EQI A++QM Q TQ A+ E+ S Sbjct: 465 EKAGGLLNQMVPSIRKTADLVQEIAAASHEQSTGLEQINNAVHQMAQTTQMTASASEELS 524 Query: 537 AASESMQMQAHKLTDLVGAFK 557 A SE M QA L DL+ F+ Sbjct: 525 ATSEEMSAQAIHLQDLMRFFQ 545 >gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sensory transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 373 bits (957), Expect = e-104, Method: Compositional matrix adjust. Identities = 224/357 (62%), Positives = 264/357 (73%), Gaps = 7/357 (1%) Query: 208 VDEFFALLD-------ARTAEQVESARAQATQMATLFYSLIAIALVVLACTLMLVYHAIR 260 +D LLD A E+ E A A + +T AIAL++ + + I Sbjct: 159 MDSMRVLLDDLDRTESALLGERAEEAAALRSVTSTTLIGGGAIALMLAVLLAWWLANNIT 218 Query: 261 NPLAHAASVARRVAHGDLTAQIDPEIKGETGELLEALTIMQSGLQTIVLDVRNGAQYINT 320 PLA A +ARRVA GDLTA+I+ + ETG LL AL M L IV VR G + I T Sbjct: 219 RPLALAVQLARRVASGDLTARIEVRSRDETGALLGALKDMNESLVRIVSGVRRGTEMIAT 278 Query: 321 SANEIASGTLDLSSRSEQQASSLEETASSMEELTSTVKQNAGNARLASELANTASGVAVR 380 +++EIA+G LDLSSR+EQQASSLEETASSMEELTSTV+QN NAR A+ +A AS +A Sbjct: 279 ASSEIATGNLDLSSRTEQQASSLEETASSMEELTSTVQQNTANAREANRMAQAASDIASS 338 Query: 381 GGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV 440 GG VVARVVDTM IN+SS++IVDII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVV Sbjct: 339 GGGVVARVVDTMEEINDSSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV 398 Query: 441 ATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAGSTMREVVESVQRVTEIITGI 500 A+EVRSLAQRSAAAAKEIK LI +SV+KVE+GS LVNEAG TMRE+VESVQRVT +I I Sbjct: 399 ASEVRSLAQRSAAAAKEIKALISDSVAKVESGSLLVNEAGDTMREIVESVQRVTNMINEI 458 Query: 501 SNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASESMQMQAHKLTDLVGAFK 557 S A +EQ SGIEQ+ QA+ QMDQVTQQNAALVEQA+AAS+S+Q QA +L V F+ Sbjct: 459 SAAGEEQRSGIEQVSQAVGQMDQVTQQNAALVEQAAAASQSLQEQAQELAQAVSVFR 515 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 357 bits (915), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 219/373 (58%), Positives = 272/373 (72%), Gaps = 5/373 (1%) Query: 187 QARELMHNHAYHVYKAKIMRPVDEFFALLDARTAEQVESARAQATQMATLFYSLIAIALV 246 +AR L + Y+ + + +D+ A+ D + + +A A + ++ +A Sbjct: 144 EARNLQRGESTKAYRTSLQQ-IDKLAAVND--KGADISNKQADAIYAQARLWIIVTVAAC 200 Query: 247 VLACTLMLVY--HAIRNPLAHAASVARRVAHGDLTAQIDPEIKGETGELLEALTIMQSGL 304 + L+ V+ I PL A SVA+RVA GDLT +I+P K ETG+LL AL M L Sbjct: 201 IGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEPAGKDETGQLLVALKSMNDSL 260 Query: 305 QTIVLDVRNGAQYINTSANEIASGTLDLSSRSEQQASSLEETASSMEELTSTVKQNAGNA 364 IV VR G Q I T++ EIASG LDLSSR+E+QASSLEETAS+MEELTSTVKQNA NA Sbjct: 261 VGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLEETASAMEELTSTVKQNADNA 320 Query: 365 RLASELANTASGVAVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILALNAA 424 R A++LA +AS VA +GG VV +VVDTMG+IN SS++I DII VIDGIAFQTNILALNAA Sbjct: 321 RQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRKIADIIGVIDGIAFQTNILALNAA 380 Query: 425 VEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAGSTMR 484 VEAARAGEQGRGFAVVA+EVRSLAQRSA AAKEIK LI+ SV++V+ GS LV AG TM Sbjct: 381 VEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIEGSVAQVDMGSKLVERAGITMS 440 Query: 485 EVVESVQRVTEIITGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASESMQM 544 EVV+SV+RVT+I+ IS+ASQEQ+ GIEQ+ AI QMD+VTQQNAALVE+A+AA++S+Q Sbjct: 441 EVVQSVRRVTDIVGEISSASQEQSEGIEQVNLAIAQMDEVTQQNAALVEEAAAAAQSLQE 500 Query: 545 QAHKLTDLVGAFK 557 QA LT VG FK Sbjct: 501 QAETLTQTVGFFK 513 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 354 bits (908), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 200/300 (66%), Positives = 245/300 (81%) Query: 258 AIRNPLAHAASVARRVAHGDLTAQIDPEIKGETGELLEALTIMQSGLQTIVLDVRNGAQY 317 I PL A V+R VA GDL+++I + ETG+LL++L M L+ IV +VR+G Sbjct: 214 GITKPLHRAVDVSRCVAEGDLSSEIAVTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDT 273 Query: 318 INTSANEIASGTLDLSSRSEQQASSLEETASSMEELTSTVKQNAGNARLASELANTASGV 377 I+T+++EIA+G LDLSSR+EQQASSL ETAS+MEEL STV+QNA NAR AS+LAN+AS V Sbjct: 274 ISTASSEIAAGNLDLSSRTEQQASSLAETASAMEELISTVRQNADNARQASQLANSASAV 333 Query: 378 AVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGF 437 A +GG VV++VVDTMGAIN SS++IVDII+VIDGIAFQTNILALNAAVEAARAGEQGRGF Sbjct: 334 AEQGGGVVSQVVDTMGAINASSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF 393 Query: 438 AVVATEVRSLAQRSAAAAKEIKTLIDNSVSKVEAGSTLVNEAGSTMREVVESVQRVTEII 497 AVVA+EVR+LAQRSA+AAKEIK LI++SV KV+ GS LV +AG+TM+EVV SV+RVT+I+ Sbjct: 394 AVVASEVRTLAQRSASAAKEIKELINDSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIV 453 Query: 498 TGISNASQEQASGIEQIQQAINQMDQVTQQNAALVEQASAASESMQMQAHKLTDLVGAFK 557 IS AS EQ GIEQI AI QMDQVTQQNAALVE+A+AA+ SMQ QA +L +V F+ Sbjct: 454 AEISAASTEQTHGIEQINLAITQMDQVTQQNAALVEEAAAAAASMQTQAGRLMQMVSVFR 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.126 0.330 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,220,299 Number of extensions: 42356 Number of successful extensions: 547 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 330 Number of HSP's successfully gapped: 72 Length of query: 568 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 478 Effective length of database: 1,193,720 Effective search space: 570598160 Effective search space used: 570598160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)