BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0228.p
         (261 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr...   533   e-153
gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro...   147   8e-37
gi|300311050|ref|YP_003775142.1| esterase_lipase superfamily pro...    66   2e-12

>gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone
           hydrolase [Herbaspirillum seropedicae SmR1]
          Length = 261

 Score =  533 bits (1372), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 258/261 (98%), Positives = 260/261 (99%)

Query: 1   MPFANVNAIRLHYQLEGDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQ 60
           MPFANVNAIRLHYQLEGDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQ
Sbjct: 1   MPFANVNAIRLHYQLEGDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQ 60

Query: 61  SEVTPGECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRVNKLILCNT 120
           SEVTPGECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQR+NKLILCNT
Sbjct: 61  SEVTPGECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNT 120

Query: 121 GAKIGTAEAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEG 180
           GAKIGTAEAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEG
Sbjct: 121 GAKIGTAEAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEG 180

Query: 181 YAAACAAVRDHDLREAIAGIRAPTLVIAGSGDVPTPPADAQFMRATIPGAQYVEFDAAHI 240
           YAAACAAVRDHDLREAIAGIRAPTL+IAGSGDVPTPPADAQFMRATIPGAQYVEFDAAHI
Sbjct: 181 YAAACAAVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQYVEFDAAHI 240

Query: 241 SNQQQAAAFIQAVVQFLTGQP 261
           SNQQQAAAF QAVVQFLTGQP
Sbjct: 241 SNQQQAAAFTQAVVQFLTGQP 261


>gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone
           hydrolase/4-carboxymuconolactone decarboxylase
           [Herbaspirillum seropedicae SmR1]
          Length = 316

 Score =  147 bits (371), Expect = 8e-37,   Method: Compositional matrix adjust.
 Identities = 104/256 (40%), Positives = 127/256 (49%), Gaps = 7/256 (2%)

Query: 11  LHYQLE----GDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQSEVTPG 66
           LHY +     G A    +VLS++LGT L MWD    Q     RV+ YD RGHG SE   G
Sbjct: 60  LHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADG 119

Query: 67  ECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRVNKLILCNTGAKIGT 126
             S+A L  D   LL  L      + GLSMGG     LA+ HP  V  L+L NT +    
Sbjct: 120 LYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPD 179

Query: 127 A--EAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEGYAAA 184
           A  EAW  RI TVR EG+  IA AV+ R+    +  A    V      L+ T A GY   
Sbjct: 180 AAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGC 239

Query: 185 CAAVRDHDLREAIAGIRAPTLVIAGSGDVPTPPADAQFMRATIPGAQY-VEFDAAHISNQ 243
           C AV   D    +  IR PTLVIAG  D  TP A AQ +   I GA+  V   A+H+S  
Sbjct: 240 CHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAV 299

Query: 244 QQAAAFIQAVVQFLTG 259
           +Q A F + V  F+ G
Sbjct: 300 EQPALFAELVCGFIDG 315


>gi|300311050|ref|YP_003775142.1| esterase_lipase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 251

 Score = 66.2 bits (160), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 47/143 (32%), Positives = 69/143 (48%), Gaps = 13/143 (9%)

Query: 1   MPFANVNAIRLHYQLEGDASLPVLVLSNSLGTSLSMWD--PQMPQWLQHFRVLRYDTRGH 58
           MPFA  +  ++HYQ+EG+   P L+L +   +SL  W          QH+RV+ +D  GH
Sbjct: 1   MPFAISDQRKIHYQVEGEG--PPLLLHHGFTSSLEAWRFFGFTDVLRQHYRVISFDALGH 58

Query: 59  GQSEVTPGECSIAQ--LGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRVNKLI 116
           GQS+      + +Q     D +A+LD   + + HF G S+GG     L    P+R+  LI
Sbjct: 59  GQSDKPHDSAAYSQHQRCRDALAVLDAAQVERTHFFGYSLGGWVGYGLVRQAPERLRSLI 118

Query: 117 LCNTGAKIGTAEAWNSRIETVRR 139
           L       G A  +  R   V R
Sbjct: 119 L-------GAAHPYEDRSWEVFR 134


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.133    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 885,150
Number of extensions: 37985
Number of successful extensions: 165
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 168
Number of HSP's successfully gapped: 3
Length of query: 261
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 178
Effective length of database: 1,226,865
Effective search space: 218381970
Effective search space used: 218381970
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)