BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0226.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310637|ref|YP_003774729.1| LysR family transcription regul... 663 0.0 gi|300312961|ref|YP_003777053.1| transcription regulator protein... 256 2e-69 gi|300309864|ref|YP_003773956.1| LysR family transcription regul... 147 9e-37 gi|300310436|ref|YP_003774528.1| LysR family transcription regul... 133 3e-32 gi|300313102|ref|YP_003777194.1| LysR family transcription regul... 129 3e-31 >gi|300310637|ref|YP_003774729.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 663 bits (1710), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/326 (100%), Positives = 326/326 (100%) Query: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA Sbjct: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 Query: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV 120 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV Sbjct: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV 120 Query: 121 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLREEKLVLAIPPSFALAGSNQ 180 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLREEKLVLAIPPSFALAGSNQ Sbjct: 121 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLREEKLVLAIPPSFALAGSNQ 180 Query: 181 RVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAESGI 240 RVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAESGI Sbjct: 181 RVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAESGI 240 Query: 241 CLIPASARLRNDLHYRLIDDARATSPIILTHRINDNAWYIEAVKRLVADMYAEHPAWLDI 300 CLIPASARLRNDLHYRLIDDARATSPIILTHRINDNAWYIEAVKRLVADMYAEHPAWLDI Sbjct: 241 CLIPASARLRNDLHYRLIDDARATSPIILTHRINDNAWYIEAVKRLVADMYAEHPAWLDI 300 Query: 301 DTNSFPATGFPIPQRKPADPGDPGEG 326 DTNSFPATGFPIPQRKPADPGDPGEG Sbjct: 301 DTNSFPATGFPIPQRKPADPGDPGEG 326 >gi|300312961|ref|YP_003777053.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 256 bits (654), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 133/296 (44%), Positives = 195/296 (65%), Gaps = 3/296 (1%) Query: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 M++R LKYF AVA+T +F+RA+E LHIAQPPLSRQI+ LEE LGV+L+ R SRP+ LT A Sbjct: 1 MELRHLKYFNAVASTLSFSRAAELLHIAQPPLSRQIRQLEEMLGVELIDRASRPIALTMA 60 Query: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV 120 G+ FYEQ LQ+L+RL+Q++ T+ I ++ +IGFV S LYG LP ++++ R PE+ Sbjct: 61 GKFFYEQTLQVLSRLEQIEEGTRVIARGRQHWFNIGFVPSALYGLLPEMIKRFRTEMPEI 120 Query: 121 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLREEKLVLAIPPSFALAGSNQ 180 +I E+ +M+Q ALKSGRIDVGFGR+ +D + + EE LV P L N Sbjct: 121 EIGFSEIVTMEQVEALKSGRIDVGFGRLPISDPDIVCETIVEEPLVAVFPLGHPLLARN- 179 Query: 181 RVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAESGI 240 +V+L ++ Q+ I+YP PRPS+AD +L + + + + P+ V E E+QTA+GLVAA G+ Sbjct: 180 KVTLAQLAAQRFILYPARPRPSYADQVLDIFSSRALRPSIVKEANEMQTAIGLVAAGVGV 239 Query: 241 CLIPASAR--LRNDLHYRLIDDARATSPIILTHRINDNAWYIEAVKRLVADMYAEH 294 L+P S + R+D+ YR + + SP+I+ R ND + +E ++ A + H Sbjct: 240 TLVPRSVQGLHRSDVVYRPLSNKGVHSPVIMNFRANDRSLLLERLRTTAATIARLH 295 >gi|300309864|ref|YP_003773956.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 147 bits (372), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 89/255 (34%), Positives = 140/255 (54%), Gaps = 4/255 (1%) Query: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 +D+ Q++ FV A NF RA+ L++ QPPLSRQI LLE LGVQL R R +RLT Sbjct: 2 LDLAQVRCFVVAATELNFRRAAALLNMTQPPLSRQIHLLEHRLGVQLFERVGRTVRLTTE 61 Query: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV 120 GRVF A ++L +Q ++ ++ + + +GF + Y +P L+ + R PE+ Sbjct: 62 GRVFLADASRLLQLSEQAESTVRRASQGKTGRVRVGFTGAAGYELIPELLMQARTSLPEI 121 Query: 121 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLREEKLVLAIPPSFALAGSNQ 180 D+ L EL S+ Q A + ID+GF R ++ + ++ EE LVLA+P ALA + Sbjct: 122 DVVLFELISVAQLEAFTANTIDIGFLRPVASRQPLELQLVDEEALVLAMPQGHALAAYD- 180 Query: 181 RVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAESGI 240 R++ ++ GQ I++ F + I+++L D+ P + + T L LV A G+ Sbjct: 181 RIATTQLDGQPFIMHSPTHGKYFYERIMAMLGAADVKPDFTQYIDQSHTILSLVRAGLGV 240 Query: 241 CLIPASARLRNDLHY 255 ++PASA+ HY Sbjct: 241 GILPASAQ---RFHY 252 >gi|300310436|ref|YP_003774528.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 300 Score = 133 bits (334), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 94/268 (35%), Positives = 143/268 (53%), Gaps = 13/268 (4%) Query: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 +++RQL+YF AVA +F RA+ +LH+ QPPLS+ I LEE LG L R R + LT A Sbjct: 7 LELRQLRYFAAVAEEMHFGRAAARLHMTQPPLSQTIAALEELLGTPLFQRTKRSVALTAA 66 Query: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV 120 G +A ++L + L + ++ LS+ F++S Y LP L+++ R HYP+V Sbjct: 67 GAALLPEAQRLLQQAAALPDLVRRAASGASGLLSLSFISSADYSILPPLLQRFRAHYPQV 126 Query: 121 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLR---EEKLVLAIPPSFALAG 177 I+L E T+ Q L G+ID G + A + +R E L+ A+P G Sbjct: 127 QIELREATTDVQLEDLLQGKIDAGLLLPPLPEKAAALLEVRPVLSEPLIAALP-----EG 181 Query: 178 SNQR---VSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLV 234 + ++ VSLK + LI++P+ P+F D ILS D + P E ++QT +GLV Sbjct: 182 AVRKRGPVSLKSLRELPLIIFPRRIAPAFHDTILSCFRDAGVTPHIGQEAIQMQTIVGLV 241 Query: 235 AAESGICLIPASAR--LRNDLHYRLIDD 260 +A G+ L+P S R + YR + D Sbjct: 242 SAGMGMALVPQSVSNLKRPGVDYRRLSD 269 >gi|300313102|ref|YP_003777194.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 84/246 (34%), Positives = 119/246 (48%), Gaps = 1/246 (0%) Query: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 +++R L YF VA T +F RA+E LHI+QPPL+RQI LE+ELG QL R+ R ++LT A Sbjct: 2 IELRHLTYFRTVAETLHFGRAAELLHISQPPLTRQIAALEKELGAQLFDRSKRAIQLTAA 61 Query: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGGLPMLVRKLRQHYPEV 120 G+ FY +I L++ K + L +GF+ ST Y LP + R YPEV Sbjct: 62 GKYFYRDTTEIFKALERAKRNVSSSSTGKSGALKVGFMMSTAYNILPSVTRHYSAAYPEV 121 Query: 121 DIQLLELTSMQQFAALKSGRIDVGFGRIRSNDATVARIVLREEKLVLAIPPSFALAGSNQ 180 D++L E ++ +IDVG + + + E L+ +P LAG Q Sbjct: 122 DMRLTEYVPNLLATDIEDEKIDVGIMYRPEDCRRLDSHTIYAEPLLAVLPRDHRLAG-RQ 180 Query: 181 RVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAESGI 240 +S E+ I P+ P D I P V E QT + LV G+ Sbjct: 181 SISAAELAEDAFISIPRMIAPVVYDLIQQHCQLHGFRPRIVLETNLQQTIVNLVGEGLGV 240 Query: 241 CLIPAS 246 L+P S Sbjct: 241 ALVPTS 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,071 Number of extensions: 38513 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 58 Number of HSP's gapped: 183 Number of HSP's successfully gapped: 58 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)