BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0225.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 795 0.0 gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 499 e-142 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 64 2e-11 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 795 bits (2052), Expect = 0.0, Method: Compositional matrix adjust. Identities = 396/398 (99%), Positives = 396/398 (99%) Query: 4 METTQHNITAAGPALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGE 63 METT HNITAAGPALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGE Sbjct: 1 METTLHNITAAGPALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGE 60 Query: 64 GTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIET 123 GTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIET Sbjct: 61 GTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIET 120 Query: 124 ALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKI 183 ALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKI Sbjct: 121 ALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKI 180 Query: 184 GRREVDADVAHVAAIKKALGGRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASP 243 GRREVDADVAHVAAIKKALG RGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASP Sbjct: 181 GRREVDADVAHVAAIKKALGDRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASP 240 Query: 244 QALARLKGRHAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDA 303 QALARLKGRHAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDA Sbjct: 241 QALARLKGRHAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDA 300 Query: 304 AGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAI 363 AGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAI Sbjct: 301 AGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAI 360 Query: 364 PHRPGLGITLDEERLQYFRRDRKQGATVTAVRPAKLAG 401 PHRPGLGITLDEERLQYFRRDRKQGATVTAVRPAKLAG Sbjct: 361 PHRPGLGITLDEERLQYFRRDRKQGATVTAVRPAKLAG 398 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 499 bits (1284), Expect = e-142, Method: Compositional matrix adjust. Identities = 251/370 (67%), Positives = 294/370 (79%), Gaps = 2/370 (0%) Query: 19 IDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGEGTTIGGLAYGAESPE 78 I+ VE L+D+PTIRPH+LSM TM+ QTL+LVR+ +DG G GE TTIGGLAYG ESPE Sbjct: 10 IETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGGLAYGEESPE 69 Query: 79 GMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIETALFDALGQRTGLPVS 138 +K+ IDTY PLLLGADA+Q+ A M +++ + NRFAKCAIETAL DAL +R G+P+S Sbjct: 70 SIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDALARRLGVPLS 129 Query: 139 QLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKIGRREVDADVAHVAAI 198 L GGR R+SLPIAWTLASGDTA+DIDEAE+MLAQRRHN+FKLKIG R V D AHVAAI Sbjct: 130 TLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAI 189 Query: 199 KKALGGRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASPQ--ALARLKGRHAIA 256 K+ALG RG+VRVDVN AW+E EA G+ L DAG +LVEQP+A+ L RL+ ++ IA Sbjct: 190 KRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLRRLRQKNRIA 249 Query: 257 IMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEG 316 IMADESL GP A+ LAR G ADVFA+KI QSGGL AQQVAAI AA +ELYGGTMLEG Sbjct: 250 IMADESLHGPQDAYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEG 309 Query: 317 AIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAIPHRPGLGITLDEE 376 AIGT+ASA FATF L WG+ELFGPLLLTEEILA PL+Y+DF L +P PGLGITLDE+ Sbjct: 310 AIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGPGLGITLDED 369 Query: 377 RLQYFRRDRK 386 R+ RRDRK Sbjct: 370 RVNALRRDRK 379 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 71/284 (25%), Positives = 118/284 (41%), Gaps = 24/284 (8%) Query: 117 AKCAIETALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEA--------- 167 A A E A DA + +P+ LLGG R+ +P + L A+ ID Sbjct: 110 AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAYLFF-KYAQHIDSPYAPDAWGEA 168 Query: 168 ---ERMLAQRRHNI-------FKLKIGRREVDADVAHVAAIKKALGGRGAVRVDVNMAWS 217 E+++AQ R I KLK G + +VA + A++KA +R+D N WS Sbjct: 169 LSEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACIKALRKAFPD-APLRIDPNGNWS 227 Query: 218 ELEAQRGLAGLVDAGCELVEQPVASPQALARLKGRHAIAIMADESLTGPASAFALARIGG 277 A R +A L+ + E P + +A L R + + + +T + Sbjct: 228 LQTAIR-MAELLGDDLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDELRRSVALDS 286 Query: 278 ADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAFATFRELQWGT 337 + GGLR QQ+A + D G+ + + I +A +H A L + Sbjct: 287 VQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYAC 346 Query: 338 ELFGPLLLTEE--ILAEPLQYQDFQLAIPHRPGLGITLDEERLQ 379 + P +E I L ++ + I PGLG+ +D+++L+ Sbjct: 347 DTHYPWQEPDEEVIQGGKLPIRNGCVQITDAPGLGVEVDQDQLR 390 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,262,456 Number of extensions: 54464 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 4 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)