BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0223.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase... 972 0.0 gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase la... 197 2e-51 gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large ... 132 8e-32 gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxido... 69 7e-13 >gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 462 Score = 972 bits (2513), Expect = 0.0, Method: Compositional matrix adjust. Identities = 460/462 (99%), Positives = 462/462 (100%) Query: 1 MNTTAAADRYDELDTLLQSALQEDKDNGVFRCRRDIFTNPDLFELEMKHLFESNWVYLAH 60 MNTTAAADRYDELDTLLQSALQEDKDNGVFRCRRDIFTNPDLFELEMKHLFESNWVYLAH Sbjct: 1 MNTTAAADRYDELDTLLQSALQEDKDNGVFRCRRDIFTNPDLFELEMKHLFESNWVYLAH 60 Query: 61 ESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVINACAHKGAMLCRRKHGNKSSFTCPFH 120 ESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVINAC+HKGAMLCRRKHGNKSSFTCPFH Sbjct: 61 ESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVINACSHKGAMLCRRKHGNKSSFTCPFH 120 Query: 121 GWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY 180 GWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY Sbjct: 121 GWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY 180 Query: 181 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATMSR 240 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATMSR Sbjct: 181 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATMSR 240 Query: 241 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD 300 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD Sbjct: 241 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD 300 Query: 301 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQY 360 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQY Sbjct: 301 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQY 360 Query: 361 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWKEGPDENARKMGMNPKLS 420 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWKEGPDENARKMGM+PKLS Sbjct: 361 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWKEGPDENARKMGMHPKLS 420 Query: 421 GERSEDEGLFVCQHHYWTEQMRKAVKKERAAAQQGSQQGAQA 462 GERSEDEGLFVCQHHYWTEQMRKAVKKERAAAQQGSQQGAQA Sbjct: 421 GERSEDEGLFVCQHHYWTEQMRKAVKKERAAAQQGSQQGAQA 462 >gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase large terminal subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 197 bits (501), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 128/410 (31%), Positives = 204/410 (49%), Gaps = 44/410 (10%) Query: 36 IFTNPDLFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVIN 95 ++T+P LFE EM +F W+++AHES+I D+ T IGRQPV++ RDK+G++N + N Sbjct: 29 LYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQPVIVVRDKTGKINVLEN 88 Query: 96 ACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTR 155 C H+GA +C + GN + FTCP+H W++ GKL + YP G D+ Sbjct: 89 RCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKL------RGLPYP-----EGYEDVIE 137 Query: 156 VA-------RFQSYRGFLFGSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSY 208 A R +SY G +F S ++++ PL ++LG + ID + Q ++G + Sbjct: 138 KADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQGEHKF 197 Query: 209 LYDGNWKMQMENGCDGYHVSSVHWNYAATMSRR-KEGGTKATDANNWSKAVAGVYGFENG 267 + GNWK+Q+EN DGYH VH + +++ E + TD + ++++ NG Sbjct: 198 SFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTDDQSITRSLG------NG 251 Query: 268 H--ILLWTNTLNPEVRPIWNQREELAARVGQARADTIVA-QTR-----------NLCLYP 313 H +++ ++ +V Q +E A V + + T+ Q R NL L+P Sbjct: 252 HSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSLHGAGFNLNLFP 311 Query: 314 NVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQYEDFFNVSGMGTAD 373 NV + F RV P+SV +T+I G E R R+R +E F G G+ D Sbjct: 312 NVAMSMSF---FRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQGPFGFGSPD 368 Query: 374 DLEEFRACQAGYAGSAVRWNDMSRGAPLWKEGPDENARKMGMNPKLSGER 423 D E + QAG ++RG L +E D+N K+ + +G R Sbjct: 369 DAEAWERVQAGSHAGRDAPILVNRG--LNREWVDDNGDKVSHSTDETGMR 416 >gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large subunit [Herbaspirillum seropedicae SmR1] Length = 426 Score = 132 bits (331), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 111/394 (28%), Positives = 173/394 (43%), Gaps = 47/394 (11%) Query: 31 RCRRDIFTNPDLFELEMKHLFE-SNWVYLAHESQIPEINDYYTTWIGRQPVVITRDKSGQ 89 R +F++ ++++LE + ++ W +LA E++IP DY +T++G PVV+TR + G Sbjct: 25 RTPYKVFSSREVYDLEQERIYRGETWSFLALEAEIPNNGDYKSTFVGDTPVVVTRTEDGA 84 Query: 90 LNAVINACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNKN- 148 L A +N C+H+GAM+CR GN SS +C +H W+F + G LL V + + K+ Sbjct: 85 LAAWVNKCSHRGAMVCRTPRGNASSHSCAYHQWSFDSRGNLLGVPFRRGQKGATGMAKDF 144 Query: 149 --GSHDLTRVARFQSYRGFLFGSLSEDVLPLEEYLG-ETRVIIDQIVDQAPNGLEVLRGN 205 H L R R SY+G +F + SE V L +Y+G E R ID+I + L R Sbjct: 145 DPKCHGL-RQLRVDSYKGLVFATFSETVEALPDYIGAEMRPWIDRIFHKPIVYLGCTRQY 203 Query: 206 SSYLYDGNWKMQMENGCDGYHVSSVHWNY-----------AATMSRRKEGGTKATDANNW 254 S NWK+ +EN D YH S +H + A +++ + G A Sbjct: 204 SK----SNWKLYLENVKDPYHASLLHLFHTTFNIFRVGMKARSLADKHHGLHSIITATKN 259 Query: 255 SKAVAGVY------GFENGHILLWTNTLNPEVRPIWNQREELAARVGQARADTIVAQTRN 308 A Y F+ G L +P V + EEL Q +V Q + Sbjct: 260 EVETADAYKQQEIRSFDEGFSL-----QDPSVLGQIKEFEELTTNHIQPIFPQLVVQQIH 314 Query: 309 LCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQYEDFFNVSG 368 L L P E+ F + ++ E R +RI+Q + +G Sbjct: 315 NTLVARQLL------------PKGPSNFELVFHFFGYEDDTPELRALRIKQ-ANLVGPAG 361 Query: 369 MGTADDLEEFRACQAGYAGSAVRWN--DMSRGAP 400 + +D E Q G A + + DM+R P Sbjct: 362 YISMEDTEATELVQRGTARDPDQCSVIDMARDNP 395 >gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 374 Score = 69.3 bits (168), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 54/204 (26%), Positives = 87/204 (42%), Gaps = 36/204 (17%) Query: 36 IFTNPDLFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVIN 95 ++ + L++ E+K LF+ Y+ HE +PE DY T + ++ R+ G + V N Sbjct: 21 VYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPWENEGRMLVRNAQG-IELVSN 79 Query: 96 ACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTR 155 C H+ A + + G+ S+ CP H WT+ G+L+ F + +L++ Sbjct: 80 VCRHRQAKMLDGR-GHASNIVCPLHRWTYDLKGELIGAP---------HFGQTPCMNLSK 129 Query: 156 VARFQSYRGFLFGSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSYLYDG--- 212 QS+ G LF + D+ + LG TR + S YLYD Sbjct: 130 TP-LQSWNGLLFEANGVDIADKLKNLGVTRDL----------------DFSGYLYDHTEV 172 Query: 213 -----NWKMQMENGCDGYHVSSVH 231 NWK +E + YHV H Sbjct: 173 HECDYNWKTFIEVYLEDYHVEPFH 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,429,938 Number of extensions: 60183 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 4 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)