BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0220.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbasp... 513 e-147 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 102 4e-23 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 99 2e-22 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 88 7e-19 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 85 7e-18 >gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 513 bits (1321), Expect = e-147, Method: Compositional matrix adjust. Identities = 257/257 (100%), Positives = 257/257 (100%) Query: 1 MKSRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVA 60 MKSRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVA Sbjct: 1 MKSRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVA 60 Query: 61 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYG 120 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYG Sbjct: 61 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYG 120 Query: 121 CHAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVAT 180 CHAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVAT Sbjct: 121 CHAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVAT 180 Query: 181 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD 240 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD Sbjct: 181 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD 240 Query: 241 ASYITGAVLPVAGGDNG 257 ASYITGAVLPVAGGDNG Sbjct: 241 ASYITGAVLPVAGGDNG 257 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 102 bits (253), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 86/256 (33%), Positives = 122/256 (47%), Gaps = 21/256 (8%) Query: 7 GKVVVVTGAAQGILRGVALGIAEEGGQVLLVDR-AEFVHDVAA--EAAGGRGKGLVADLE 63 GKV ++TGA+ GI R AL A G ++L R + + VAA A GGR + D+ Sbjct: 6 GKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGDVG 65 Query: 64 SYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYGCHA 123 A + + A+ +G +DI IN G +P A+ +P Q + +L G Sbjct: 66 EAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGARC 125 Query: 124 VLPQLLQQGRGVIVNVSS--NATRGIR-RVPYSAAKGGVNALTAALAMEYGELGIRVVAT 180 +P +L +G G IV SS ++ G+ Y AAK G+ L +A +Y GIR A Sbjct: 126 QIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRANAL 185 Query: 181 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD 240 PGG V + GD QQ+Q W G + KR + EE A LFLAS Sbjct: 186 LPGG-------VDTDMGGDPQQKQ-W-------AAGLHAMKRIAQPEEIAAAALFLASPM 230 Query: 241 ASYITGAVLPVAGGDN 256 AS++TG L GG++ Sbjct: 231 ASFVTGTALYADGGNS 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 99.4 bits (246), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 81/259 (31%), Positives = 124/259 (47%), Gaps = 26/259 (10%) Query: 4 RFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDR-AEFVHDVAAEAAGGRGKGLVADL 62 RF GKVV+VTGAA GI A ++EG +VLL DR A + V R D+ Sbjct: 11 RFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDV 70 Query: 63 ESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPT----- 117 + + +D A++R+G++D+L++ G +A+ T++ E + T Sbjct: 71 SHHEQVRQLVDFAIERFGQLDVLVSDAG------VFAEGNVTEVSPEDWHRVQATNVNGV 124 Query: 118 LYGCHAVLPQLLQQGRGVIVNVSSNA--TRGIRRVPYSAAKGGVNALTAALAMEYGELGI 175 YG LP L++ RG IVNV+S + Y+A+KG V LT A+A+++G G+ Sbjct: 125 FYGAREALPH-LEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGV 183 Query: 176 RVVATAPGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILF 235 R+ A P T D + + + +++ R + E A I F Sbjct: 184 RINAVCPSLTHTA-------MTADMADDPPLLDKFAERIA----LGRGADPLEIAAVITF 232 Query: 236 LASDDASYITGAVLPVAGG 254 LAS DAS++ G LPV GG Sbjct: 233 LASPDASFVNGVNLPVDGG 251 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 87.8 bits (216), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 85/255 (33%), Positives = 126/255 (49%), Gaps = 23/255 (9%) Query: 8 KVVVVTGAA--QGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVADLESY 65 KV V+TG A G+ A +AE+G +V+++D A+ AA G + GLVAD+ Sbjct: 6 KVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVTDK 65 Query: 66 AGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYGCHAVL 125 A + A +Q++GRIDIL+N G ++ + T D + SL TLY AVL Sbjct: 66 ASCETAAAAILQQFGRIDILVNNAGITQPVKTL-EITGADYDRILDVSLRGTLYMSQAVL 124 Query: 126 PQLLQQGRGVIVNVSSNATR---GIRRVP-YSAAKGGVNALTAALAMEYGELGIRVVATA 181 P + Q G I+ +SS + + GI P YSAAK GV L A+A E+G GIRV + Sbjct: 125 PAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRVNSIT 184 Query: 182 PG--GTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASD 239 PG GT ++ +++++ + E + R ++ +FL SD Sbjct: 185 PGLIGTDIIKGKL-------TEEKKAEIAETIP-------LARLGRADDIGGACVFLGSD 230 Query: 240 DASYITGAVLPVAGG 254 + Y TG L V GG Sbjct: 231 LSQYCTGITLDVNGG 245 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 84.7 bits (208), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 80/255 (31%), Positives = 117/255 (45%), Gaps = 24/255 (9%) Query: 8 KVVVVTGAAQGILRGVALGIAEEGGQVLL----VDRAEFVHDVAAEAAGGRGKGLVADLE 63 +V +VTG A GI ++ +A +G VL+ +D A D A AAG R L DL Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTAD-ALVAAGHRAAPLQMDLG 67 Query: 64 SYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYGCHA 123 S AA + YGR D+L+N G A R + Y + ++ L Sbjct: 68 SPESIAAAFAHIEKDYGRCDVLVNNAGVA-RTYSFLDYPLENWLQTMNVNVTGVLLAGQH 126 Query: 124 VLPQLLQQGRGVIVNVSSNATRGIR----RVPYSAAKGGVNALTAALAMEYGELGIRVVA 179 +++Q G IVN+SS GIR R Y +K V LT +A+E + GI V + Sbjct: 127 AARLMVKQQWGRIVNISS--ISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNS 184 Query: 180 TAPGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASD 239 APG P P S+Q + V +RY T +E + + +LA++ Sbjct: 185 VAPG-----PVDTPMTQAMHSEQTRESYYRLVP-------MRRYGTTDEIASAVCYLAAE 232 Query: 240 DASYITGAVLPVAGG 254 +++YITG V+PV GG Sbjct: 233 ESAYITGHVIPVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 886,236 Number of extensions: 39152 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 41 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)