BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0218.p (455 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 897 0.0 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 244 1e-65 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 177 1e-45 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 126 3e-30 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 119 7e-28 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 897 bits (2318), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/455 (100%), Positives = 455/455 (100%) Query: 1 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA Sbjct: 1 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 Query: 61 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG 120 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG Sbjct: 61 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG 120 Query: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA Sbjct: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 Query: 181 AAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAGA 240 AAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAGA Sbjct: 181 AAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAGA 240 Query: 241 PMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG 300 PMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG Sbjct: 241 PMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG 300 Query: 301 VVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQIL 360 VVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQIL Sbjct: 301 VVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTIGTQIL 360 Query: 361 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPGLVA 420 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPGLVA Sbjct: 361 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPGLVA 420 Query: 421 ALAMGLFMLRYRHGSQASHAAPAAKAPPAAASRSV 455 ALAMGLFMLRYRHGSQASHAAPAAKAPPAAASRSV Sbjct: 421 ALAMGLFMLRYRHGSQASHAAPAAKAPPAAASRSV 455 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 244 bits (622), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 151/429 (35%), Positives = 251/429 (58%), Gaps = 7/429 (1%) Query: 1 MKTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGA 60 + T+ V Q+++ RF F + ++ LC L L+ DG+D+ G V P ++ +W +S G Sbjct: 9 LPTVHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGP 68 Query: 61 LGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAG 120 + LFGM+ G+ + P+ DR GR+ + +S + F++ + T+ + V RF+ G Sbjct: 69 VFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITG 128 Query: 121 LGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFA 180 G G +MPNA+AL+ E++P R T + ++ G+++G + ++P +GW ++F+ Sbjct: 129 FGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWV 188 Query: 181 -AAVPLVL-LPVILWKLPESVGFLL--RQGRLEQARAMLTQVDPQARIATDTQLVQADVK 236 AVPLVL L +++W LPES+ FL+ R+ R + AR L ++DP I T++V + K Sbjct: 189 GGAVPLVLGLAMLVW-LPESIQFLVLHRRPRTQVAR-WLRKLDPNIVIDDKTEVVVKETK 246 Query: 237 GAGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLAL 296 G P+ LF+ RA T+++W+ F L+ +Y L +WLP L+ AGYS ++ +L Sbjct: 247 AEGMPVAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSL 306 Query: 297 NFGGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTP-VLYLLIIVAGATTI 355 GGV+G + G RF +V+ S F + ++IAL+G + P +L++ +IVAG + Sbjct: 307 QVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIV 366 Query: 356 GTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAI 415 G Q + A FYP +RSTG+GWA G+GR G++VGP++GG L+A FL A+ Sbjct: 367 GGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAV 426 Query: 416 PGLVAALAM 424 P L++AL + Sbjct: 427 PALISALTI 435 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 177 bits (450), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 131/402 (32%), Positives = 201/402 (50%), Gaps = 9/402 (2%) Query: 2 KTLDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGAL 61 +T+D+ +++D F+ F +V+CL AL +V DG+D + G +P L+KEW ++ Sbjct: 10 QTVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPA 69 Query: 62 GSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGL 121 + L GM G+ G ADR GR+ + S +F V+T L F+ T + RF AGL Sbjct: 70 VAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGL 129 Query: 122 GCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAA 181 G GG +P++ + E++P R R+ V LGG+L +LP++GW+ +F Sbjct: 130 GIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIG 189 Query: 182 AVPLVLLPVILW-KLPESVGFLLRQ-GRLEQARAMLTQVDPQARIATDTQLVQADVKG-- 237 + V V+LW +PES +L R R ++ R +L ++ ++ DT A + Sbjct: 190 GLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRP--VSADTGFADAREQAIE 247 Query: 238 AGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALN 297 A LF +A T+ +WV+FF LL VY+ SWLP ++A G S + + L A N Sbjct: 248 TRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYN 307 Query: 298 FGGVVGAIGGGWLGDRF--TLPKVVVSF-FTVGTVAIALLGFNSPTPVLYLLIIVAGATT 354 FGGV GA+ RF LP ++ T + + L S T +L + I + G Sbjct: 308 FGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLHGLFV 367 Query: 355 IGTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLG 396 Q +YA A YP +R+TG A GR GAI+ G Sbjct: 368 NAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAG 409 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 126 bits (317), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 115/434 (26%), Positives = 200/434 (46%), Gaps = 20/434 (4%) Query: 10 IDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGALGSYALFGM 69 I+ F+++H + +FD + VLPVL+ W LS Q G L + G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 70 MFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGLGCGGLMPN 129 + GA FG +A+R GR +++ L SV + + S + RFL G+G GG +P Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 130 AVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAAAVPLVLLP 189 A +NE + R + + LG + A +G +++P FGW+ MF +P +L+ Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGGIPGILVA 212 Query: 190 VILWKLPESVGFLLRQGRLEQARAMLTQVD---PQARI--ATDTQLVQADVK----GAGA 240 +++ +LPES +L+ +GRL+ A ++ ++ P+ + TD Q ++ + GAG Sbjct: 213 LLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGK 272 Query: 241 PML----HLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGY-SLGSSLSFLLA 295 LF T+++W+ + + + +WLP L + + L SL Sbjct: 273 RQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASV 332 Query: 296 LNFGGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTI 355 N +G L DR + ++ F + +ALLG ++ + + A + Sbjct: 333 SNVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAV 392 Query: 356 --GTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVF 413 T +LLY T + YP +R+ G G A+ R+ + P + G ++A + FL+F Sbjct: 393 MGTTTVLLYLYTPEIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGH-GIATVFLMF 451 Query: 414 A---IPGLVAALAM 424 A + GL+AA M Sbjct: 452 AGVTVLGLLAAWRM 465 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 119 bits (297), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 114/431 (26%), Positives = 198/431 (45%), Gaps = 6/431 (1%) Query: 1 MKTL--DVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQA 58 M TL V Q + A KF + + + L+ + D + G P + K + L+ QA Sbjct: 1 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA 60 Query: 59 GALGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFL 118 G+ GM+ GAF+FG LADRIGR+ + + V+ + S FA T R L Sbjct: 61 LQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLL 120 Query: 119 AGLGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLP--NFGWQS 176 G+G GG +P +M E+ P R + ++ S +++G + A L + + N W+ Sbjct: 121 TGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRV 180 Query: 177 MFFAAAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQARIATDTQLVQADVK 236 +FF +P ++ V+ + +PES +L R G+ + AR +L +V +T +Q +V Sbjct: 181 IFFVTGLPALVGVVLRFYVPESPMYLNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEVV 240 Query: 237 GAGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLAL 296 PM LF +L +++++ + Y + +LP + S G+ FL+ L Sbjct: 241 -ERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFL 299 Query: 297 NFGGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTIG 356 + G + +R+ +V F + V L ++ ++ + +G Sbjct: 300 ALVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLG 359 Query: 357 TQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFL-VFAI 415 T LYA T + YP +R++G+G A V R G + P + +MA+ L + L F + Sbjct: 360 TWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLV 419 Query: 416 PGLVAALAMGL 426 G VA L + + Sbjct: 420 AGAVAILCVDV 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,208,732 Number of extensions: 46402 Number of successful extensions: 407 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 386 Number of HSP's successfully gapped: 29 Length of query: 455 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 367 Effective length of database: 1,203,190 Effective search space: 441570730 Effective search space used: 441570730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)