BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0205.p (207 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoa... 426 e-121 gi|300310740|ref|YP_003774832.1| isochorismatase family protein ... 70 2e-13 gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily prot... 62 3e-11 gi|300310060|ref|YP_003774152.1| nicotinamidase-like protein [He... 55 5e-09 >gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 426 bits (1094), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/207 (100%), Positives = 207/207 (100%) Query: 1 MHKEVETYQRQGFGQTLEMKPPFGLLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHAR 60 MHKEVETYQRQGFGQTLEMKPPFGLLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHAR Sbjct: 1 MHKEVETYQRQGFGQTLEMKPPFGLLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHAR 60 Query: 61 QQGWPVAHSRIVFADDDADRNIFGMKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVP 120 QQGWPVAHSRIVFADDDADRNIFGMKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVP Sbjct: 61 QQGWPVAHSRIVFADDDADRNIFGMKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVP 120 Query: 121 SAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPH 180 SAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPH Sbjct: 121 SAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPH 180 Query: 181 EANLFDMAQKYAAVMPLDEAMQVVGQR 207 EANLFDMAQKYAAVMPLDEAMQVVGQR Sbjct: 181 EANLFDMAQKYAAVMPLDEAMQVVGQR 207 >gi|300310740|ref|YP_003774832.1| isochorismatase family protein [Herbaspirillum seropedicae SmR1] Length = 227 Score = 69.7 bits (169), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 56/177 (31%), Positives = 79/177 (44%), Gaps = 17/177 (9%) Query: 25 LLIVDFVNSFADPQQFGG--GN----IPQAIERTRSLLAHARQQGWPVAHSRIVFADDDA 78 L+++D F + FG GN + + +LLA ARQ V H+R D + Sbjct: 20 LVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALARQTQMLVVHTRESHLPDLS 79 Query: 79 D----RNIFGMKVPGM-------VTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTS 127 D + + G G+ L PG+ I+PQL P GE+V+ K AF+ T Sbjct: 80 DCPRTKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLTPMAGEIVIDKPGKGAFYATD 139 Query: 128 LAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANL 184 L L + G+ LLVAG T CV+ S+ +A G+ LVV D H A L Sbjct: 140 LHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVVEDACASYFPAFHRATL 196 >gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 62.4 bits (150), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 45/151 (29%), Positives = 67/151 (44%), Gaps = 15/151 (9%) Query: 49 IERTRSLLAHARQQGWPVAHSRIVFADDDAD---------RNI------FGMKVPGMVTL 93 IE R +LA R G+ + H+R D +D R I G P L Sbjct: 56 IEPIRKVLAAMRAGGYTIIHTREGHRPDLSDLPANKRWRSRQIGANGVGIGDAGPCGRIL 115 Query: 94 KEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVAGAVTSGCVRA 153 PG I+P+LAP GE+++ K +F T L L G++ +++ G T CV Sbjct: 116 VRGEPGWEIIPELAPIAGEIIIDKPGKGSFCATDLEMILHTRGIRNIVLTGITTDVCVHT 175 Query: 154 SVVDAMSWGFRPLVVSDCVGDRALGPHEANL 184 ++ +A GF +++SDC G H A L Sbjct: 176 TMREANDRGFECVMLSDCCGATDYNNHLAAL 206 >gi|300310060|ref|YP_003774152.1| nicotinamidase-like protein [Herbaspirillum seropedicae SmR1] Length = 202 Score = 55.1 bits (131), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 52/183 (28%), Positives = 79/183 (43%), Gaps = 26/183 (14%) Query: 25 LLIVDFVNSFADPQQFGGGNIPQAIERTRSLLAHARQQGWPVAHSRIVFADDDADRNIFG 84 L+I+D + + G I A+ LL HAR PV H + Sbjct: 25 LVIIDHQTEYTTGRAPLAG-IGAAVAEVALLLEHARAAAAPVIH-------------VVH 70 Query: 85 MKVPGMVTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTSLAPWLTQHGVQTLLVAG 144 PG P AI+ + P GE V+ K +P++F GT+L L + G ++L++AG Sbjct: 71 HGKPGGAMFDPQGPHVAIIDGVQPQPGEAVITKGLPNSFAGTTLDETLKKTGRKSLIIAG 130 Query: 145 AVTSGCVRASVVDAMSWGFRPLV---------VSDCVGDRALGPHEAN---LFDMAQKYA 192 T CV A+V A+ G+ V + D +G AL E L ++A +YA Sbjct: 131 FATHMCVSATVRSALDHGYACTVVAAACATRDLPDPLGGPALSAAEIQRVALAELADRYA 190 Query: 193 AVM 195 V+ Sbjct: 191 TVV 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 707,821 Number of extensions: 30575 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 4 Length of query: 207 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 126 Effective length of database: 1,236,335 Effective search space: 155778210 Effective search space used: 155778210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)