BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0200.p (346 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 686 0.0 gi|300313057|ref|YP_003777149.1| porin signal peptide protein [H... 182 5e-47 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 148 7e-37 gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 119 4e-28 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 116 2e-27 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 686 bits (1769), Expect = 0.0, Method: Compositional matrix adjust. Identities = 345/346 (99%), Positives = 345/346 (99%) Query: 1 MKKAWAVGVAGLLCAGGALAQSNVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGF 60 MKKAWAVGVA LLCAGGALAQSNVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGF Sbjct: 1 MKKAWAVGVASLLCAGGALAQSNVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGF 60 Query: 61 KGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFL 120 KGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFL Sbjct: 61 KGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFL 120 Query: 121 MIARLDAFAGATYASFLNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAPRNGLS 180 MIARLDAFAGATYASFLNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAPRNGLS Sbjct: 121 MIARLDAFAGATYASFLNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAPRNGLS 180 Query: 181 TAMLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGGAYDYGSGKVFLGYYRGNNLGAA 240 TAMLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGGAYDYGSGKVFLGYYRGNNLGAA Sbjct: 181 TAMLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGGAYDYGSGKVFLGYYRGNNLGAA 240 Query: 241 ASANTPGSYYSAYSLSANYRLTGQATLGAGYGWAKDSTGAGRNARQFSLIGTYDLSRRTM 300 ASANTPGSYYSAYSLSANYRLTGQATLGAGYGWAKDSTGAGRNARQFSLIGTYDLSRRTM Sbjct: 241 ASANTPGSYYSAYSLSANYRLTGQATLGAGYGWAKDSTGAGRNARQFSLIGTYDLSRRTM 300 Query: 301 LYATYAHLANNNGASFSLAGAAPITKNAPVSGGTVNGVQVGIRHLF 346 LYATYAHLANNNGASFSLAGAAPITKNAPVSGGTVNGVQVGIRHLF Sbjct: 301 LYATYAHLANNNGASFSLAGAAPITKNAPVSGGTVNGVQVGIRHLF 346 >gi|300313057|ref|YP_003777149.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 182 bits (461), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 134/372 (36%), Positives = 190/372 (51%), Gaps = 40/372 (10%) Query: 11 GLLCA--GGALAQS--NVTLYGVIDTYMGYTNATGKGSVASLDSGGYQASRVGFKGSEDL 66 GLL A G ALAQS NVTLYG +D Y+GY ++ S+ +L+ G SR+G KG EDL Sbjct: 13 GLLLAASGSALAQSASNVTLYGTLDQYLGYMRSSSGKSLWALNDGAILRSRIGLKGYEDL 72 Query: 67 GGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNE-LGELRLGRQNSPGFLMIARL 125 GGG F +ENGF++D+GA D TRLF+RQSWVG+ GE R+GRQNS F + + Sbjct: 73 GGGYATTFTLENGFSADTGAQADGTRLFDRQSWVGIKTPAAGEFRIGRQNSVPFFIGGAI 132 Query: 126 DAFAGATYASFLNNVSAYTPRYDNVIGYLSPVMRGFRVQAYYGLGEQTAP---RNG---L 179 D TY S +N R+DN + + SP G ++ +Y + E R G L Sbjct: 133 DYTERTTYGSIINTFGV-PSRFDNDLSWKSPRWAGLQIDLHYAMPETAGEGLGRRGVYQL 191 Query: 180 STAMLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGGAYDYGSGKVFLGYYRG----- 234 + G Y +G L +A ++ + + + YDYG GKV+L Y R Sbjct: 192 GIDYINGPYRAGYAGLMAKPNAGGLYDNAVQYHNVYLN--YDYGPGKVYLAYVRSNNTTA 249 Query: 235 -----------------NNLGAAASANTPGSYYSAYSLSANYRLTGQATLGAGYGWAKDS 277 NN A AN +Y+ Y +SA+YRLT Q +GA YG D+ Sbjct: 250 NTNGNNAATILNNVSNPNNFFAGNDANVQ-RFYNVYQISADYRLTPQLRIGALYGVMVDT 308 Query: 278 TGAGRNARQFSLIGTYDLSRRTMLYATYAHLANNNGASFSLAGAAPITKN---APVSGGT 334 + A+ ++ YDLS+RT LY ++ N A + +G+A N A ++G + Sbjct: 309 SHKEGGAQGGNVGAYYDLSKRTTLYGFANYMKNQANAGWRFSGSAGPGSNLAGADINGKS 368 Query: 335 VNGVQVGIRHLF 346 + G+Q+GI H F Sbjct: 369 LVGLQMGILHRF 380 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 148 bits (373), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 127/372 (34%), Positives = 188/372 (50%), Gaps = 55/372 (14%) Query: 16 GGALAQSNVTLYGVIDTYMGY-TNATGKG-SVASLDSGGYQASRVGFKGSEDLGGGLRAN 73 G A AQSNVT+YG++DT + Y +NA +G S +D+G SR+GFKG+EDLGGGL+A Sbjct: 17 GAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLKAM 76 Query: 74 FQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFLMIARLDAFAGATY 133 FQ+ENGF+SD+GAM LF RQSWVGL G++R+GRQN+P F ++A D F T Sbjct: 77 FQLENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQNTPLFDLLA--DHFDPLTV 134 Query: 134 ASFLNN----VSAYTPRYDNVIGY---LSPVMRGFRVQAYYGLGEQTAPRNGLSTAMLAG 186 ++ +N +A R N++ Y PV A + GE+ S A Sbjct: 135 GNYASNSWLPAAASLVRSPNMLRYDGDFGPVA----ASASWAFGEKAGSVRTGSQFSSAL 190 Query: 187 EYESGPLYLGVSSSAQNSANDNITIRSTF--AGGAYDYGSGKVFLGYYR----------- 233 Y +GPL LG Q ++ N +++ T +YD+G KVF G+ Sbjct: 191 RYTAGPLALG-GGYQQTVSSTNSSLKHTTWNLSASYDFGVAKVFGGWLNIKDDSGLTGSV 249 Query: 234 -------GNNLGAAASANTPGS----YYSAYSLSANYRLTGQATLGAGYGWAKD------ 276 N A +AN + + + L ++Y+ T TL + + + Sbjct: 250 MAADGDVSNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLTGAFYYDRSKNVSYV 309 Query: 277 STGAGRNARQFSLIG--TYDLSRRTMLYATYAHLANNNGASFSLAGAAPITKNAPVSGGT 334 STG + ++++L+G Y LS+RT LY T + + A + L +A KN+ + Sbjct: 310 STGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYELTNSAN-GKNSLTT--- 365 Query: 335 VNGVQVGIRHLF 346 V VGIRH+F Sbjct: 366 ---VGVGIRHIF 374 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 119 bits (298), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 125/369 (33%), Positives = 162/369 (43%), Gaps = 70/369 (18%) Query: 21 QSNVTLYGVIDTYMGYT---NATGKGSVASLDSGGYQASRVGFKGSEDLGGGLRANFQME 77 QS+VT+YG+IDT + YT NA G G SR+GFKG+EDLGGGL+A F +E Sbjct: 24 QSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGFKGTEDLGGGLQAMFVLE 83 Query: 78 NGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFLMIARLDAFAGATYASFL 137 +GFA D+GAM RLF RQS+VGL N G+L LGRQ + +L + D +A + Sbjct: 84 SGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPNLFA--I 141 Query: 138 NNVSAYTP--RYDNVIGYLSPVMRGFRVQAYYGLGEQTAPRNGLSTAMLAGEYESGP--- 192 ++ AY P R DN IGYL GF V A Y G A G + GE Sbjct: 142 GSIDAYLPNARSDNAIGYLGN-FSGFTVGATYSFGRDAAGPAGPAATNCPGEVAGNSKAC 200 Query: 193 ----------------------LYLGVSSSAQNSANDNITIRSTFAG----GAYDYGSGK 226 LY +S +++DN R T G G G G Sbjct: 201 RQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRVTLNGYFMLGDVKIGGGV 260 Query: 227 VFLGYYRGNNLGAAASANTPGSYY---SAYSLSANYRLTGQATLGAGYGWAKDSTGAGRN 283 V AAA+ NT Y +Y LS L Q + D T + Sbjct: 261 V------DRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLDVKNSPNDVTLS--V 312 Query: 284 ARQFSLIGTYDLSRRTMLYATYAHLANNNGASFSLAGAAPITKNAPVSGGTVN------G 337 AR TY+LS+RT +Y + ++ N +S +L +GGTV G Sbjct: 313 ARL-----TYNLSKRTAVYGSLGYIKNGGTSSVALD-----------AGGTVGAGKNQLG 356 Query: 338 VQVGIRHLF 346 V GIRH F Sbjct: 357 VMTGIRHTF 365 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 109/324 (33%), Positives = 151/324 (46%), Gaps = 33/324 (10%) Query: 1 MKKAWAVGVAGLLCAGGAL---AQSNVTLYGVIDTYMGYTNATGK-----GSVASLDSGG 52 MKK V + AGGA AQS+V++YG+ID + Y ++ K + +DSG Sbjct: 1 MKKTLLVAA---MLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGI 57 Query: 53 YQASRVGFKGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGN-ELGELRL 111 Q SR+GFKG+EDLGGG+ A F +E GF +D G++ LF RQS+VGLG+ + G L + Sbjct: 58 AQGSRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSV 117 Query: 112 GRQ-----NSPGFLMIARLDA--------FAGATYASFLNNVSAYTPRYDNVIGYLSPVM 158 GRQ N G + L A A + + LNN R +N I Y S Sbjct: 118 GRQYDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKF 177 Query: 159 RGFRVQAYYGLGEQTAPRNGLSTAMLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGG 218 GF A GLGE N T G+Y++GP ++ + + + + R G Sbjct: 178 GGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFSTRIYGGGA 237 Query: 219 AYDYGSGKVFLGYYRGNNLGAAASANTPGSYYSAYSLSANYRLTGQATLGAGYGWAKDST 278 Y G+ F + N +A A T Y L Y LT L A Y + K + Sbjct: 238 RYQIGAFTPFGVVTQVKNTNTSAKATT-------YELGTVYALTPLWDLAAAYQFQKRNQ 290 Query: 279 GAGRNARQFSLIGTYDLSRRTMLY 302 G G NA+ Y +S+RT LY Sbjct: 291 GVG-NAQALVATVDYKISKRTDLY 313 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,026,638 Number of extensions: 42798 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 14 Length of query: 346 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 260 Effective length of database: 1,212,660 Effective search space: 315291600 Effective search space used: 315291600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)