BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0198.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 791 0.0 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 102 5e-23 gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 70 3e-13 gi|300311950|ref|YP_003776042.1| 2-polyprenyl-6-methoxyphenol hy... 65 1e-11 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 791 bits (2044), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/384 (99%), Positives = 384/384 (100%) Query: 1 VSNSPRIAVVGAGLGGAATAALLLQEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIG 60 +SNSPRIAVVGAGLGGAATAALLLQEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIG Sbjct: 1 MSNSPRIAVVGAGLGGAATAALLLQEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIG 60 Query: 61 IEDEMNRQGSRPDFWYSRHWQTGDVLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALP 120 IEDEMNRQGSRPDFWYSRHWQTGDVLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALP Sbjct: 61 IEDEMNRQGSRPDFWYSRHWQTGDVLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALP 120 Query: 121 SERLSFGKCLTKVEDRGDVVILSFADGTTEEADIVIGADGVNSRIREELLGVEPPKYAGY 180 SERLSFGKCLTKVEDRGDVVILSFADGTTEEADIVIGADGVNSRIREELLGVEPPKYAGY Sbjct: 121 SERLSFGKCLTKVEDRGDVVILSFADGTTEEADIVIGADGVNSRIREELLGVEPPKYAGY 180 Query: 181 LAHRAVFPTPELKAGMLPFDACVKWWSDDRHMMVYFVTSKANELYYVTGVPVEKWDLNDR 240 LAHRAVFPTPELKAGMLPFDACVKWWSDDRHMMVYFVTSKANELYYVTGVPVEKWDLNDR Sbjct: 181 LAHRAVFPTPELKAGMLPFDACVKWWSDDRHMMVYFVTSKANELYYVTGVPVEKWDLNDR 240 Query: 241 WLPSSKEEMRETFHGWHPTVQALVDATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMK 300 WLPSSKEEMRETFHGWHPTVQALVDATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMK Sbjct: 241 WLPSSKEEMRETFHGWHPTVQALVDATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMK 300 Query: 301 PHMAQGAAMAIEDGAMLARCFKEVGVSHYADAFALYEANRAARASKVQRISHDNTWLRTN 360 PHMAQGAAMAIEDGAMLARCFKEVGVS+YADAFALYEANRAARASKVQRISHDNTWLRTN Sbjct: 301 PHMAQGAAMAIEDGAMLARCFKEVGVSNYADAFALYEANRAARASKVQRISHDNTWLRTN 360 Query: 361 EDPSWCFGYDVFTEPLVSPSKAAA 384 EDPSWCFGYDVFTEPLVSPSKAAA Sbjct: 361 EDPSWCFGYDVFTEPLVSPSKAAA 384 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 102 bits (254), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 99/347 (28%), Positives = 149/347 (42%), Gaps = 17/347 (4%) Query: 6 RIAVVGAGLGGAATAALLLQEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEM 65 RI ++G G G + A L + G V + E + GAGI +GP ++ L+++G+ + Sbjct: 7 RILIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAF 66 Query: 66 NRQGSRPDFWYSRHWQTGDVLAQIP---LGDYAVKHYGASYLTVHRGDFHELLIKALPSE 122 R+G+ D G +A++P L V GA V + A Sbjct: 67 LREGAAGD-GVRLCLPHGPQVAELPTPRLASPDVPGGGAILRPVLARILADATRAAGADV 125 Query: 123 RLSFGKCLTKVEDRGDVVILSFADGTTEEADIVIGADGVNSRIREELLGVEP-PKYAGYL 181 RL G + V + G V + F DG T D+VIGADG+ S++R L P P+Y+G Sbjct: 126 RL--GCTFSAVREVGHKVEVDFTDGQTRRYDLVIGADGLYSKLRMHLFPHAPKPRYSGQA 183 Query: 182 AHRAVFPTPELKAGMLPFDACVKWWSDDRHMMVYFVTSKANELYYVTGVPVEKWDLNDRW 241 RAV P P+ C W V V+ L+ PV + + Sbjct: 184 VWRAVLPRPQ------EIVTCTMWMGPRIKPGVNPVSKDEMYLFVTEPRPVNEHVDPTTF 237 Query: 242 LPSSKEEMRETFHGWHPTVQALVDATVEVTKWSLLERDPLPL-WSRGRLVLLGDACHPMK 300 + + + E T++ +D + + LE LP W +GR+VL+GDA H Sbjct: 238 VSHLRGLLEEFSAPVLKTIREQLDDNARII-FRPLEGLLLPRPWYQGRVVLIGDAVHATT 296 Query: 301 PHMAQGAAMAIEDGAMLARCFKEVGVSHYADAFALYEANRAARASKV 347 PH+A GA + IED +LA + G A A +E R R V Sbjct: 297 PHLASGACIGIEDALVLADELERHGT--VPQALAAFEERRWERCRMV 341 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 70.1 bits (170), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 85/379 (22%), Positives = 145/379 (38%), Gaps = 48/379 (12%) Query: 7 IAVVGAGLGGAATAALLLQEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMN 66 +A+ GAG G A A L + G V + E+ + +G+G+ + P + VL+ +G+ + Sbjct: 6 VAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGLFSRIA 65 Query: 67 RQGSRPDFWYSRHWQTGDVLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPSERLSF 126 G R D Y +G + + A YG + VHR +L++A E + Sbjct: 66 ALGQRIDRLYGADALSGKTVLDVRYDAIAGGRYG---IAVHRAALFGVLLEAAQQEGIEV 122 Query: 127 --GKCLTKVEDRGDVVILSFADGTTEEA-DIVIGADGVNSRIREELLGVEPPKYAGY--- 180 G+ + +E G L+ ADG D+VI A G S +R L P+ Y Sbjct: 123 ETGQDIGNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRPLPYGAF 182 Query: 181 -----------LAHRAVFPTPELKAGMLPFDACVKWWSDDRHMMVYFVTSKANELYYVTG 229 H ++ + M+ + D M+ +F +++ + + + Sbjct: 183 WVSLRAQPGLSFGHNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFWSTRPDRVESLKQ 242 Query: 230 VPVEKWDLNDRWLPSSKEEMRETFHGWHPTVQ---ALVDATVEVTKWSLLERDPLPLWSR 286 + W ++ H P A +D ++T + LPL Sbjct: 243 AGLSAW--------------KDGVHALWPECAPYLAQIDGFEQMT-LARYGHHTLPLPYG 287 Query: 287 GRLVLLGDACHPMKPHMAQGAAMAIEDGAMLARCFKEVGVSHYADAFALYEANRAARASK 346 RL ++GDA H P + QGA MA+ D L+ ++ A Y A+R Sbjct: 288 ERLAIIGDAAHSTSPQLGQGANMALLDARALSHALQQ--SETLEQALPRYAASRRRHVRT 345 Query: 347 VQRIS--------HDNTWL 357 Q +S D+ WL Sbjct: 346 FQAMSAMFTPFYQSDSVWL 364 >gi|300311950|ref|YP_003776042.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 407 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 85/359 (23%), Positives = 152/359 (42%), Gaps = 34/359 (9%) Query: 27 GFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSR--PDFWYSRHWQTGD 84 G+ V V EQA G + + + +RR+ D + R G R PD +H Sbjct: 33 GWRVLVVEQAEELRDGGHMMGLSGPGLATVRRM---DLVARLGERAYPDM--GQHIYRDR 87 Query: 85 VLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPSERLS--FGKCLTKVEDRGDVVIL 142 +I +Y G +LT+ RG + L ALP+ ++ FG + + ++ V + Sbjct: 88 RGREILRINYREALAGIEWLTLRRGVLVQELRAALPTRDIAWRFGVTVQEALNQASSVRV 147 Query: 143 SFADGTTEEADIVIGADGVNSRIREELLGVEPP-------KYAGYLAHRAVFPTPELKAG 195 S +DG+ EAD++I ADGV+S +R + +YA Y + + + Sbjct: 148 SLSDGSCHEADLLIAADGVHSSLRRRHFAADANCLHPLGYRYASYEVQDHLLLGQDFLSY 207 Query: 196 MLPFDACVKWWSDDRHMMVYFVTSKANELYYVTGVPVEKWDLNDRWLPSSKEEMRETFHG 255 P + D + V ++ E +V P ++ L + +F G Sbjct: 208 AEPGIQTEYYGLCDGRLAALHVW-RSREAGFVP--PAQRHSL-------LRTCTARSFTG 257 Query: 256 WHPTVQALVD-ATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMKPHMAQGAAMAIEDG 314 T++A+ + + + +L+E LP W +GR++LLGDA H + QGA +A+ Sbjct: 258 VRATLEAVENPQEIVIDDLALVE---LPSWRQGRILLLGDAAHSLSLISGQGAGVALASA 314 Query: 315 AMLARCFKEVGVSHYADAFALYEANRAARASKVQ----RISHDNTWLRTNEDPSWCFGY 369 ++LA + A + + E+ A +++ R+ H + L PS G+ Sbjct: 315 SILAEELERAAAGGVAPSSSALESALARHDQRLRPTILRLQHRSRKLAPMYVPSSRVGF 373 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,267,323 Number of extensions: 54635 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 4 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)