BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0197.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 855 0.0 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 135 1e-32 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 117 2e-27 gi|300313868|ref|YP_003777960.1| major facilitator superfamily p... 114 2e-26 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 104 1e-23 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/438 (99%), Positives = 437/438 (99%) Query: 1 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA 60 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA Sbjct: 1 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA 60 Query: 61 LQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLL 120 LQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLL Sbjct: 61 LQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLL 120 Query: 121 TGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRV 180 TGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRV Sbjct: 121 TGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRV 180 Query: 181 IFFVTGLPALVGVVLRFYVPESPMYLNRSGKSDAARLVLERVAKVNGSSTPIPPLQPEVV 240 IFFVTGLPALVGVVLRFYVPESPMYLNRSGKSDAAR VLERVAKVNGSSTPIPPLQPEVV Sbjct: 181 IFFVTGLPALVGVVLRFYVPESPMYLNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEVV 240 Query: 241 ERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLA 300 ERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLA Sbjct: 241 ERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLA 300 Query: 301 LVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLGT 360 LVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLGT Sbjct: 301 LVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLGT 360 Query: 361 WAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLVA 420 WAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLVA Sbjct: 361 WAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLVA 420 Query: 421 GAVAILCVDVESRNRALE 438 GAVAILCVDVESRNRALE Sbjct: 421 GAVAILCVDVESRNRALE 438 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 135 bits (339), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 127/399 (31%), Positives = 193/399 (48%), Gaps = 31/399 (7%) Query: 20 FQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFG 79 FQ + + L + D IGF+ P++ K +G+T VGM +G+ G Sbjct: 26 FQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPAVAAGLVGMGIGSAFAG 85 Query: 80 RLADRIGRRPVLMGAVVIDACFGVAS---AFAPDFTWLLALRLLTGIGVGGTLPVDYTMM 136 ADR GRR ++G+V + FGVA+ AF+PD T + LR G+G+GG LP TM Sbjct: 86 LFADRFGRRMAIIGSVFV---FGVATSLIAFSPDVTTIAILRFCAGLGIGGALPSSTTMT 142 Query: 137 AEFLPSARRGRWLVMLESFWAIGTI-CLAVLALVAVSWGND-----AWRVIFFVTG-LPA 189 AEF P+ RR + TI C+ + ++A + N WR +F + G LP Sbjct: 143 AEFSPARRR--------TLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIGGLLPV 194 Query: 190 LVGVVLRFYVPESPMYLNRS-GKSDAARLVLERVAKVNGSSTPIPPLQPEVVE-RKPMSA 247 V+L F +PESP YL R + D R +L+R+++ + T + + +E R A Sbjct: 195 AFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIETRAGFGA 254 Query: 248 LFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLALVQLPGY 307 LF++ R +++L++A+ + +A Y F +LP L EG L + G Sbjct: 255 LFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYNFGGVFGA 314 Query: 308 ALSAYGVERWG-RKPTLVGFLVLSAVGCMLYSL---GKDPMLVVGSTLLLSFSLLGTWAA 363 L A + R+G R P L+ L +A L SL + +LVVG L F + + Sbjct: 315 LLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLHGLF-VNAVQST 373 Query: 364 LYAFTPEVYPTDLRASGMGTAGAVARFGGL---FAPAIV 399 +YA +YPT +RA+G A A R G + FA A+V Sbjct: 374 MYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALV 412 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 114/427 (26%), Positives = 196/427 (45%), Gaps = 6/427 (1%) Query: 1 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA 60 M TL V Q + A KF + + + L+ + D + G P + K + L+ QA Sbjct: 1 MKTL--DVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQA 58 Query: 61 LQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLL 120 G+ GM+ GAF+FG LADRIGR+ + + V+ + S FA T R L Sbjct: 59 GALGSYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFL 118 Query: 121 TGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRV 180 G+G GG +P +M E+ P R + ++ S +++G + A L + + N W+ Sbjct: 119 AGLGCGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLP--NFGWQS 176 Query: 181 IFFVTGLPALVGVVLRFYVPESPMYLNRSGKSDAARLVLERVAKVNGSSTPIPPLQPEVV 240 +FF +P ++ V+ + +PES +L R G+ + AR +L +V +T +Q +V Sbjct: 177 MFFAAAVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQARIATDTQLVQADVK 236 Query: 241 -ERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFL 299 PM LF +L +++++ + Y + +LP + S G+ FL+ L Sbjct: 237 GAGAPMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLAL 296 Query: 300 ALVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLG 359 + G + +R+ +V F + V L ++ ++ + +G Sbjct: 297 NFGGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVAGATTIG 356 Query: 360 TWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLV 419 T LYA T + YP +R++G+G A V R G + P + +MA+ L + L F + Sbjct: 357 TQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFL-VFAI 415 Query: 420 AGAVAIL 426 G VA L Sbjct: 416 PGLVAAL 422 >gi|300313868|ref|YP_003777960.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 114 bits (285), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 80/241 (33%), Positives = 126/241 (52%), Gaps = 12/241 (4%) Query: 19 KFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAK--TFGLTVPQALQTGTMFFVGMLLGAF 76 ++ +R+ + G+ W+ D ++V +G + + T GL Q TG+++ G ++GA Sbjct: 20 RWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQVGWTGSLYIAGAVVGAL 79 Query: 77 VFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMM 136 VFGRL DR+GR+ + M + + +ASAFA DF W RL TG+G+GG + + Sbjct: 80 VFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRLATGLGIGGEYAAINSAI 139 Query: 137 AEFLPSARRGR-WLVMLESFWAIGTICLAVLALVAVSWG----NDAWRVIFFVTGLPALV 191 E +P+ RGR LV+ SFW IG A L+LV + WRV F + + A+ Sbjct: 140 DELVPARVRGRVNLVINASFW-IGAALGAGLSLVILDERVLGPVMGWRVCFLLGAVLAVA 198 Query: 192 GVVLRFYVPESPMYLNRSGKSDAARLVLERV-AKV---NGSSTPIPPLQPEVVERKPMSA 247 V++R +VPESP +L G A V+ + A+V +G+ +P P R P A Sbjct: 199 IVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTLPALPTAAPGESSRSPGQA 258 Query: 248 L 248 + Sbjct: 259 V 259 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 117/446 (26%), Positives = 199/446 (44%), Gaps = 37/446 (8%) Query: 19 KFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVF 78 ++ + +I G L DA LS+ F P + + L+ Q ++G ++GA F Sbjct: 40 RWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFF 99 Query: 79 GRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAE 138 G +A+R GR P V + + + A F L R L GIGVGG +PV T + E Sbjct: 100 GWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINE 159 Query: 139 FLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDAWRVIFFVTGLPALVGVVLRFY 198 + RG++ V+ E + +G + A + V W +F V G+P ++ +L Sbjct: 160 LSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVP--RFGWEYMFLVGGIPGILVALLITR 217 Query: 199 VPESPMYLNRSGKSDAARLVLERVAKVNGSSTPIPPLQPEV------------------V 240 +PESP +L G+ D A E++ + +STP L + Sbjct: 218 LPESPRWLIARGRLDDA----EKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKR 273 Query: 241 ERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLA 300 +R LFS R R++ ++L W G+ +LP + ++ + + ++ Sbjct: 274 QRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVS 333 Query: 301 LVQLPGYAL-SAYGVERWGRKP-TLVGFLVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLL 358 V L A ++R GR+ + F++ A+ +L L + V +++++ Sbjct: 334 NVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAVM 393 Query: 359 GTWAA-LYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAF 417 GT LY +TPE+YPT +RA G G A + R APA+V V+A H + + F Sbjct: 394 GTTTVLLYLYTPEIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGH-----GIATVF 448 Query: 418 LVAGAVAILCV-----DVESRNRALE 438 L+ V +L + +E+ NR+LE Sbjct: 449 LMFAGVTVLGLLAAWRMIETTNRSLE 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,337,830 Number of extensions: 57699 Number of successful extensions: 416 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 387 Number of HSP's successfully gapped: 21 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)