BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0192.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 641 0.0 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 251 8e-68 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 206 2e-54 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 58 1e-09 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 641 bits (1653), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/318 (98%), Positives = 314/318 (98%) Query: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 121 MAYRLWAEEKRFTLHYFARAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTACLEA 180 MAYRLWAEEKRFTLHYF RAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLT CLEA Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 181 GSKEAHVYTCGPAPFMDAVIKCASATRSEEAIHLEHFQAVPAAPAAAGGDGAFEVKLASS 240 GSKEAHVYTCGPAPFMDAV+KCASATRSEEAIHLEHFQA PAAPAAAGGDGAFEVKLASS Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCASATRSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLASS 240 Query: 241 GKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND 300 GKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND Sbjct: 241 GKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND 300 Query: 301 QICTCVSRARSGRLVLDL 318 QICTCVSRARSG LVLDL Sbjct: 301 QICTCVSRARSGMLVLDL 318 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 251 bits (640), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 148/319 (46%), Positives = 188/319 (58%), Gaps = 5/319 (1%) Query: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 +++ V +E DI + LV DGA LPA+ GAHIDV P G++RQYSLC ++ Sbjct: 4 LQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQHR 63 Query: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Y I V R+ SRGGS A+H+ +K G L + PRN FAL A LL GIGVTP+L Sbjct: 64 YQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFAL-VPAQHSLLLAGGIGVTPILC 122 Query: 121 MAYRLWAEEKRFTLHYFARAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTACLEA 180 MA L A F +HY AR+ AF + + + FAA V HY A + L A L Sbjct: 123 MAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALLAQ 182 Query: 181 GSKEAHVYTCGPAPFMDAVIKCASATRSEEA-IHLEHFQAVPAAPAAAGGDGAFEVKLAS 239 H+Y CGPA F+ V+ A A EA +H E+F A AP A G+GAFEVKLAS Sbjct: 183 ADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGA---APVKADGNGAFEVKLAS 239 Query: 240 SGKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAN 299 SG+V IP ++ +L G+ + SC +GICGTC+ +LEGEPDHRD L+ +E+AAN Sbjct: 240 SGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAN 299 Query: 300 DQICTCVSRARSGRLVLDL 318 DQ C SRARS LVLDL Sbjct: 300 DQFTPCCSRARSALLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 206 bits (525), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 140/326 (42%), Positives = 178/326 (54%), Gaps = 10/326 (3%) Query: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 + L V + DIR LV DGA LP + PGAH+ + G++R YSLC + R Sbjct: 16 LMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDC 75 Query: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Y IAVKRE SRGGSA LH+ V+ G + P N F L A LL AGIG+TPL A Sbjct: 76 YLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHA 135 Query: 121 MAYRLWAEEKRFTLHYFARAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTACLEA 180 MA L + +R LHYFAR+ HAAF E A + + H + A+ EH + A L A Sbjct: 136 MAAELATQGRRHRLHYFARSLAHAAF--FHELAAGSQGMSLHLGLDGAETEHAIAAALAA 193 Query: 181 GSKEAHVYTCGPAPFMDAVIKCAS-ATRSEEAIHLEHFQAVPAAPAAA-------GGDGA 232 + + +Y CGP+PF+DA + A A E+ IH E F A A A D Sbjct: 194 APRSSPLYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAPDST 253 Query: 233 FEVKLASSGKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLS 292 FE+ L SG Q+ G IV A+ G+VI TSC EG CG+C +LEG+P HRD+ LS Sbjct: 254 FELVLQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDSVLS 313 Query: 293 KKEKAANDQICTCVSRARSGRLVLDL 318 E+A+ +I CVSR RLVLDL Sbjct: 314 AAERASGRRILPCVSRCAGTRLVLDL 339 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 63/221 (28%), Positives = 87/221 (39%), Gaps = 25/221 (11%) Query: 2 KLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHID---VTGPTGIMRQYSLCGPLEDR 58 IV E+ +I SF V DG L + PG +I V RQYSL L Sbjct: 157 NFIVSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQA 216 Query: 59 CG---YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGV 115 Y I+VKRE + S LHD + G +E+ P F L AS L G+G+ Sbjct: 217 GQPPQYRISVKREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGI 275 Query: 116 TPLLAMAYRLWAEEKRFTLHYFARAREHAAFADLLENAPFAA-----HVRFHYAVMPAQ- 169 TP+++M + +H+ AR A + AA +RF Y P Q Sbjct: 276 TPMMSMLAAALPSGR--PIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYE-QPRQG 332 Query: 170 ---------MEHYLTACLEAGSKEAHVYTCGPAPFMDAVIK 201 +E L G+ + Y GP FM A+ + Sbjct: 333 DAAPHATGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKR 373 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 990,229 Number of extensions: 41135 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 100 Number of HSP's successfully gapped: 4 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)