BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0190.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase la... 897 0.0 gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase... 197 2e-51 gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large ... 144 1e-35 gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxido... 72 6e-14 >gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase large terminal subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 897 bits (2317), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/428 (100%), Positives = 428/428 (100%) Query: 1 MNAPLRFHPRKTDSSYADLVQDAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNP 60 MNAPLRFHPRKTDSSYADLVQDAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNP Sbjct: 1 MNAPLRFHPRKTDSSYADLVQDAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNP 60 Query: 61 GDFKTAQIGRQPVIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLD 120 GDFKTAQIGRQPVIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLD Sbjct: 61 GDFKTAQIGRQPVIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLD 120 Query: 121 GKLRGLPYPEGYEDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLF 180 GKLRGLPYPEGYEDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLF Sbjct: 121 GKLRGLPYPEGYEDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLF 180 Query: 181 MKQGAGFPIKVQGEHKFSFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTD 240 MKQGAGFPIKVQGEHKFSFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTD Sbjct: 181 MKQGAGFPIKVQGEHKFSFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTD 240 Query: 241 DQSITRSLGNGHSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSL 300 DQSITRSLGNGHSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSL Sbjct: 241 DQSITRSLGNGHSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSL 300 Query: 301 HGAGFNLNLFPNVAMSMSFFRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQG 360 HGAGFNLNLFPNVAMSMSFFRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQG Sbjct: 301 HGAGFNLNLFPNVAMSMSFFRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQG 360 Query: 361 PFGFGSPDDAEAWERVQAGSHAGRDAPILVNRGLNREWVDDNGDKVSHSTDETGMREAYA 420 PFGFGSPDDAEAWERVQAGSHAGRDAPILVNRGLNREWVDDNGDKVSHSTDETGMREAYA Sbjct: 361 PFGFGSPDDAEAWERVQAGSHAGRDAPILVNRGLNREWVDDNGDKVSHSTDETGMREAYA 420 Query: 421 MWKKMMEK 428 MWKKMMEK Sbjct: 421 MWKKMMEK 428 >gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 462 Score = 197 bits (500), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 121/375 (32%), Positives = 191/375 (50%), Gaps = 43/375 (11%) Query: 29 LYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQPVIVVRDKTGKINVLEN 88 ++T+P LFE EM +F W+++AHES+I D+ T IGRQPV++ RDK+G++N + N Sbjct: 36 IFTNPDLFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVIN 95 Query: 89 RCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKL------RGLPYP-----EGYEDVIE 137 C H+GA +C + GN + FTCP+H W++ GKL + YP G D+ Sbjct: 96 ACSHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTR 155 Query: 138 KADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQGEHKF 197 A R +SY G +F S ++++ PL ++LG + ID + Q ++G + Sbjct: 156 VA-------RFQSYRGFLFGSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSY 208 Query: 198 SFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTDDQSITRSLG------NG 251 + GNWK+Q+EN DGYH VH + +++ E + TD + ++++ NG Sbjct: 209 LYDGNWKMQMENGCDGYHVSSVHWNYAATMSRR-KEGGTKATDANNWSKAVAGVYGFENG 267 Query: 252 HSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSLHGAGFNLNLFP 311 H +++ ++ +V Q +E A V + + T+ Q R NL L+P Sbjct: 268 H--ILLWTNTLNPEVRPIWNQREELAARVGQARADTIVA-QTR-----------NLCLYP 313 Query: 312 NVAMSMSF---FRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQGPFGFGSPD 368 NV + F RV P+SV +T+I G E R R+R +E F G G+ D Sbjct: 314 NVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQYEDFFNVSGMGTAD 373 Query: 369 DAEAWERVQAGSHAG 383 D E + QAG +AG Sbjct: 374 DLEEFRACQAG-YAG 387 >gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large subunit [Herbaspirillum seropedicae SmR1] Length = 426 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 124/433 (28%), Positives = 202/433 (46%), Gaps = 48/433 (11%) Query: 16 YADLVQ----DAVVDP-SLYTDPALFEAEMDKIFY-KTWIWVAHESEIKNPGDFKTAQIG 69 YA LVQ D P +++ +++ E ++I+ +TW ++A E+EI N GD+K+ +G Sbjct: 12 YAPLVQFPRTDGARTPYKVFSSREVYDLEQERIYRGETWSFLALEAEIPNNGDYKSTFVG 71 Query: 70 RQPVIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKLRGLPYP 129 PV+V R + G + N+C HRGA VC +GNA+ +C YH WS+ G L G+P+ Sbjct: 72 DTPVVVTRTEDGALAAWVNKCSHRGAMVCRTPRGNASSHSCAYHQWSFDSRGNLLGVPFR 131 Query: 130 EGYEDVIEKA-DLP-----LQSLRCESYHGMIFASFNQEIEPLSDFLG-HAKKWID-LFM 181 G + A D L+ LR +SY G++FA+F++ +E L D++G + WID +F Sbjct: 132 RGQKGATGMAKDFDPKCHGLRQLRVDSYKGLVFATFSETVEALPDYIGAEMRPWIDRIFH 191 Query: 182 KQGAGFPIKVQGEHKFSFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTDD 241 K PI G + K NWK+ LEN D YH ++H F ++ + M + Sbjct: 192 K-----PIVYLGCTRQYSKSNWKLYLENVKDPYHASLLH-LFHTTFNIFRVGMKA----- 240 Query: 242 QSITRSLGNGHSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSLH 301 RSL + H + + +V+ Q+ E S P V ++ Sbjct: 241 ----RSLADKHHGLHSIITATKNEVETADAYKQQEIRSFDEGFSLQDP--SVLGQIKEFE 294 Query: 302 GAGFN--LNLFPNVAM----SMSFFRVLHPVSVGETQIRHVALGM-DGGPEIANRERLRI 354 N +FP + + + R L P ++ G D PE+ LRI Sbjct: 295 ELTTNHIQPIFPQLVVQQIHNTLVARQLLPKGPSNFELVFHFFGYEDDTPEL---RALRI 351 Query: 355 HE-HFQGPFGFGSPDDAEAWERVQAGSHAGRDAPILVNRGLNREWVDDNGDKVSHSTDET 413 + + GP G+ S +D EA E VQ G+ D +++ DN D+ E+ Sbjct: 352 KQANLVGPAGYISMEDTEATELVQRGTARDPDQCSVIDMA------RDNPDQEDTLITES 405 Query: 414 GMREAYAMWKKMM 426 +R+ + ++K+M Sbjct: 406 LIRKFWMGYQKLM 418 >gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 374 Score = 72.4 bits (176), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 59/219 (26%), Positives = 106/219 (48%), Gaps = 20/219 (9%) Query: 14 SSYADLVQ-DAVVDPSLYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQP 72 +++A L + +A + +Y D L++ E+ ++F + +V HE + GD+ T + Sbjct: 5 ATFAKLARSNAQLPVHVYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPWENEG 64 Query: 73 VIVVRDKTGKINVLENRCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKLRGLPYPEGY 132 ++VR+ G I ++ N CRHR A + + +G+A+ CP H W+Y L G+L G P+ G Sbjct: 65 RMLVRNAQG-IELVSNVCRHRQAKMLDG-RGHASNIVCPLHRWTYDLKGELIGAPH-FGQ 121 Query: 133 EDVIEKADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQ 192 + + PLQ S++G++F + +I LG + +D +G+ Sbjct: 122 TPCMNLSKTPLQ-----SWNGLLFEANGVDIADKLKNLGVTRD-LDF-----SGYLYDHT 170 Query: 193 GEHKFSFKGNWKIQLENTTDGYHFPVVHK---TFLSSVD 228 H+ + NWK +E + YH H F+S D Sbjct: 171 EVHECDY--NWKTFIEVYLEDYHVEPFHPGLGNFVSCSD 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,477,019 Number of extensions: 65443 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 4 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)