BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0186.p (278 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 558 e-160 gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspir... 107 1e-24 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 106 2e-24 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 103 1e-23 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 98 9e-22 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 558 bits (1439), Expect = e-160, Method: Compositional matrix adjust. Identities = 277/278 (99%), Positives = 278/278 (100%) Query: 1 MNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQYAMG 60 MNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQYAMG Sbjct: 75 MNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQYAMG 134 Query: 61 ALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGETAGN 120 ALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGETAGN Sbjct: 135 ALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGETAGN 194 Query: 121 NNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPFGVITQVK 180 NNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPFGV+TQVK Sbjct: 195 NNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPFGVVTQVK 254 Query: 181 NTNTSAKATTYELGTVYALTPLWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYV 240 NTNTSAKATTYELGTVYALTPLWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYV Sbjct: 255 NTNTSAKATTYELGTVYALTPLWDLAAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYV 314 Query: 241 GAVYDRDKGYNAYPVFGGGIQSATGNQTALRLGMRHRF 278 GAVYDRDKGYNAYPVFGGGIQSATGNQTALRLGMRHRF Sbjct: 315 GAVYDRDKGYNAYPVFGGGIQSATGNQTALRLGMRHRF 352 >gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 372 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 96/313 (30%), Positives = 146/313 (46%), Gaps = 49/313 (15%) Query: 1 MNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQYAMG 60 + A F LETGF DDG++ G LF R+S VGL ++G+L+VGRQ D++ IG QY Sbjct: 74 LAAIFQLETGFAGDDGAMATPGTLFDRKSVVGLAG-RFGTLTVGRQTDYLEEIGQQYTSF 132 Query: 61 ALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGETAGN 120 + FG G AAH +L+ G R+NNS+++++A++GG G GE AG+ Sbjct: 133 QI-----FGGG-GVRAAHFNSLDR--ITGARSNNSLRFDTAEYGGLRGSFYYGAGEVAGS 184 Query: 121 NNAGRTVSATGQYDNGP-------FSGALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPF 173 +G+++ G + G F LA + G + + GA YQ G F Sbjct: 185 TRSGQSLGVGGNFRRGNVGVGAAYFQSKLASDALPARAGDTELKTFTLGASYQAGPAKLF 244 Query: 174 GVITQVKN-------------TNTSAKATTYELGTVYALTPLWDLAAAYQFQKRN--QGV 218 +Q++ ++ +A ++G YAL L A+ + + + Sbjct: 245 AAWSQIRRPTQQPLASSGLVAITSATRANVLDVGVDYALDSKLHLLASVIHDRADLSRAA 304 Query: 219 GNAQALVAT-----VDYKISKRTDLYVGAVYDRDKGYNAYPVFGGGIQSAT--------G 265 G A T VD +SKRTD+Y A+ R + A V GI SA Sbjct: 305 GGATRGSTTQVNLGVDLLLSKRTDVY--AMASRQQ---ARDVLNPGIISAAYSSAPADDS 359 Query: 266 NQTALRLGMRHRF 278 +Q LR+G+RH+F Sbjct: 360 SQNVLRVGLRHKF 372 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 105/330 (31%), Positives = 138/330 (41%), Gaps = 66/330 (20%) Query: 1 MNAFFTLETGFGADDGSLGQG---GLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQY 57 ++A F LE GF D G+L LF R+S VGL +GS+ +GRQ DF I Sbjct: 75 LSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAG-NFGSVLLGRQTDFADTIS--- 130 Query: 58 AMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGET 117 + + FG G A H N G RTNNSI Y +A GF+ M G GET Sbjct: 131 ---SYTSVQDFG-GWTGAAGH----NLDRLEGTRTNNSISYTTANLNGFTGNLMYGFGET 182 Query: 118 AGNNNAGRTVSATGQYDNGPFSGALAYTDIK----------------------DSTGSFS 155 AG +AG+ G+YDNGP + Y K + GS + Sbjct: 183 AGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNNGSTA 242 Query: 156 TRIYGGGARYQIGAFTPFGVITQVK-NTNTSA----------------------KATTYE 192 ++ A YQ G +G ++VK + NT A KA YE Sbjct: 243 QKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYE 302 Query: 193 LGTVYALTPLWDLAAAYQFQKRN-QGVGNAQALVAT---VDYKISKRTDLYVGAVYDRDK 248 +GT Y+L+P L AA + GV L DY +SKRTDLY Y + Sbjct: 303 IGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAFLAYSKAT 362 Query: 249 GYNAYPVFGGGIQSATGNQTALRLGMRHRF 278 N V GNQ A+ +G+RH+F Sbjct: 363 DLNNPGV--ADSSGTDGNQGAVAVGIRHKF 390 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 103 bits (257), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 111/323 (34%), Positives = 149/323 (46%), Gaps = 59/323 (18%) Query: 1 MNAFFTLETGFGADDGSLGQGGL------LFGRQSFVGLGSTQWGSLSVGRQYDFMANIG 54 ++A F LETGF +DDG L QG LF R+S VGL S +G++ +GRQ DF I Sbjct: 79 LSAVFNLETGFTSDDGGL-QGSDAVTSSNLFRRKSVVGL-SGGFGTVLLGRQTDFADTIS 136 Query: 55 GQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGL 114 A + FG G+ A++ G++LN G RTNNS+ Y +A GF+ + G Sbjct: 137 ------AYTAVADFG-GVVANS--GSSLNR--LQGTRTNNSVSYTTANLSGFTGNLIYGF 185 Query: 115 GETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKD-STGSFSTRIYGGG----------- 162 GE AG +AG+ G+YDNGP + Y K ST S ++ I G Sbjct: 186 GEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANTALGVA 245 Query: 163 -----------ARYQIGAFTPFGVITQVKN----TNTSA--------KATTYELGTVYAL 199 A YQ G + ++VK T T A KA TYE+GT Y+L Sbjct: 246 GNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATLANAQKADTYEIGTAYSL 305 Query: 200 TPLWDLAAAYQFQKRN-QGV---GNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPV 255 +P L AA + GV G + DY +SKRTDLY R P Sbjct: 306 SPSLKLLAAVDHSRATFNGVNTKGKLTQISLGADYFLSKRTDLYAFVSNMRASDIQN-PG 364 Query: 256 FGGGIQSATGNQTALRLGMRHRF 278 GG + +QTA+ G+RH+F Sbjct: 365 VTGGATGSDASQTAIITGIRHKF 387 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 97.8 bits (242), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 88/260 (33%), Positives = 117/260 (45%), Gaps = 45/260 (17%) Query: 1 MNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANIGGQYAMG 60 + A FTLE GF D G GQG L++GRQ+ VGL S +G++ VGRQ D + +IGG Sbjct: 76 LKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGL-SGNFGTVLVGRQNDPLNDIGG----- 129 Query: 61 ALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVGLGETAGN 120 ++ AG FG H L+ G R NNSI+YNS F G SF + G GE G Sbjct: 130 -IASAGGFGV---PGTVHSKGLDR--TDGQRLNNSIRYNSPNFNGLSFSGIYGFGEQVGR 183 Query: 121 NNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFSTRIYGGGARYQIGAFTPFGVITQVK 180 GR++ QY NGP S L Y F +++ GG Sbjct: 184 AVGGRSIGLGSQYVNGPLSLGLGY---------FQSKMGGGSD----------------G 218 Query: 181 NTNTSAKATTYELGTVY----ALTPLWDLAAAYQF--QKRNQGVGNAQALVATVDYKISK 234 +++ +AKA G W LAAAYQF K + + +A + K+ Sbjct: 219 SSDVNAKAACNGAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKV-- 276 Query: 235 RTDLYVGAVYDRDKGYNAYP 254 R D GA+YD G P Sbjct: 277 RKDNNFGAMYDAKTGTGYNP 296 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 961,020 Number of extensions: 44574 Number of successful extensions: 168 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 14 Length of query: 278 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 194 Effective length of database: 1,222,130 Effective search space: 237093220 Effective search space used: 237093220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)