BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0185.p (508 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310677|ref|YP_003774769.1| two component transmembrane sen... 1035 0.0 gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 140 2e-34 gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains ... 137 1e-33 gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sens... 83 6e-17 gi|300309416|ref|YP_003773508.1| two component sensor histidine ... 71 2e-13 >gi|300310677|ref|YP_003774769.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 1035 bits (2677), Expect = 0.0, Method: Compositional matrix adjust. Identities = 505/508 (99%), Positives = 507/508 (99%) Query: 1 MPEPTAAPEDDPIRFGLDDFRRMMDALPMCIILHDAQTKSILWANPSACQVLGFTLEELL 60 MPEPTAAPEDDPIRFGLDDFRRMMDALPMCIILHDAQTKSILWANPSACQVLGFTLEELL Sbjct: 1 MPEPTAAPEDDPIRFGLDDFRRMMDALPMCIILHDAQTKSILWANPSACQVLGFTLEELL 60 Query: 61 PLKAPDMSRQSHEYRREIGRKWLHDATLHGSNMVEWCYRAKNGEEILSEAIATLVPLTER 120 PLKAPDMSRQSHEYRREIGRKWLHDATLHGSNMVEWCYRAKNGEEILSEAIATLVPLTER Sbjct: 61 PLKAPDMSRQSHEYRREIGRKWLHDATLHGSNMVEWCYRAKNGEEILSEAIATLVPLTER 120 Query: 121 DVIMVQFRNIAREEQIRREMKKLESRLKEFMQDSDEGVAVLRPDGGIEYLSDAGRKLLGV 180 DVIMVQFRNIAREEQIRREMKKLESRLKEFMQDSDEGVAVLRPDGGIEYLSDAGRKLLGV Sbjct: 121 DVIMVQFRNIAREEQIRREMKKLESRLKEFMQDSDEGVAVLRPDGGIEYLSDAGRKLLGV 180 Query: 181 RPGQNPPKNFVAQCLPDSQEELQELLTLALPDAISFPLRYRIARKDGLVRWHHAVCRYIE 240 RPGQNPPKNFV QCLPDSQEELQELLTLALPDAISFPLRYRIARKDGLVRWHHAVCRYIE Sbjct: 181 RPGQNPPKNFVEQCLPDSQEELQELLTLALPDAISFPLRYRIARKDGLVRWHHAVCRYIE 240 Query: 241 IENDLKGHLLHFHDITDQVEAEEARRRDQAALEYLARHNAMGEMATAIAHELSQPLAAIR 300 IENDLKGHLLHFHDITDQVEAEEARRRDQAALEYLARHNAMGEMATAIAHELSQPLAAIR Sbjct: 241 IENDLKGHLLHFHDITDQVEAEEARRRDQAALEYLARHNAMGEMATAIAHELSQPLAAIR 300 Query: 301 NFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHAAAIIKSVREYVVKLEQSEGLIDL 360 NFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHAAAIIKSVREYVVKLEQSEGLIDL Sbjct: 301 NFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHAAAIIKSVREYVVKLEQSEGLIDL 360 Query: 361 NEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEKVLIGQVILNLAFNAIEEMSHFPQ 420 NEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEKVLIGQVILNLAFNAIEEMSHFP+ Sbjct: 361 NEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEKVLIGQVILNLAFNAIEEMSHFPE 420 Query: 421 SQRRVCISLARKDQQALLTVADEGRGIDAGHRERLFDGFFSSKVSGNGIGLALCKNIIAR 480 +QRRVCISLARKDQQALLTVADEGRGIDAGHRERLFDGFFSSKVSGNGIGLALCKNIIAR Sbjct: 421 NQRRVCISLARKDQQALLTVADEGRGIDAGHRERLFDGFFSSKVSGNGIGLALCKNIIAR 480 Query: 481 HRGDIWAQPAQVRGTRFCFTLPLVQRHN 508 HRGDIWAQPAQVRGTRFCFTLPLVQRHN Sbjct: 481 HRGDIWAQPAQVRGTRFCFTLPLVQRHN 508 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 140 bits (354), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 106/350 (30%), Positives = 176/350 (50%), Gaps = 18/350 (5%) Query: 164 DGGIEYLSDAGRKLLGVRP----GQNPPKNFVAQCLPDSQEELQELLTLALPDAISFPLR 219 +G I Y++ A K++G G+ PP + A P++ +E QE + L + Sbjct: 326 EGRITYVNPAFCKMVGFAAEELVGKIPPMPYWA---PEAMDEYQERFSNVLAGRATPQFE 382 Query: 220 YRIARKDGLVRWHHAVCRYIEIENDLK--GHLLHFHDITDQVEAEEARRRDQAALEYLAR 277 R+DG R+ + ++ D K G + DI+++ AE+ R + L+ AR Sbjct: 383 TIFQRRDG-ERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASAR 441 Query: 278 HNAMGEMATAIAHELSQPLAAIRNFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHA 337 +MGE+A+ +AHEL+QPLAAI ++ G++ + +A + LE Q A Sbjct: 442 LASMGEIASTLAHELNQPLAAISSYTTGALNLMQQGQA---DPKALQPALEKIHAQAQRA 498 Query: 338 AAIIKSVREYVVKLEQSEGLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEK 397 +I+SV ++V K E + + + +++ V IE++A + V L++Q V ++ Sbjct: 499 GHVIRSVHQFVKKREPARQRVQMRQLIDNVAPLIELQAQQFFVVLQLQVPRSLPPVLGDQ 558 Query: 398 VLIGQVILNLAFNAIEEMSHFPQSQR--RVCISLARKDQQA---LLTVADEGRGIDAGHR 452 +L+ QVILNL N I+ M+ P ++R RV + R + A ++V D+G GI Sbjct: 559 ILLEQVILNLTRNGIQAMAAVPSARRILRVAAEVTRNEDGAESVTVSVTDQGHGIPDDVA 618 Query: 453 ERLFDGFFSSKVSGNGIGLALCKNIIARHRGDIWAQPAQVRGTRFCFTLP 502 RLF FFS+K G G+GL +C+ I H G + GT F F+LP Sbjct: 619 ARLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLP 668 >gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains containing sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 524 Score = 137 bits (346), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 112/354 (31%), Positives = 178/354 (50%), Gaps = 35/354 (9%) Query: 169 YLSDAGRKLLGVRPGQN--PPKNFVAQCLPDSQEELQELLTLALPDAISFPLRYRIARKD 226 Y SD ++ G+ PG+N + F A PD ++E+ E + A+ + + +RI R+D Sbjct: 187 YCSDELCRIYGLAPGRNCITYEEFAAIMHPDDRQEVVETVNAAVAMGGTIRVEHRICRQD 246 Query: 227 -GLVRWHHAVCRYIEIENDLKGHLLHFHDITDQVEAEEARRRDQAALEYLARHNAMGEMA 285 G VR+ + + I + ++ DIT + + E A R Q +E ++R N +G++ Sbjct: 247 TGEVRYLSGIGKPIWRDGTFTEYVGTATDITVRRQNEYAIRAAQIDMERVSRANTVGQLT 306 Query: 286 TAIAHELSQPLAAI-------RNFLEGSVLRLNHSE-ALR------EQAGQIIWGLESAG 331 +IAHE++QPL +I +L+ V L H+ LR ++AG II GL Sbjct: 307 ASIAHEINQPLMSIVANAGASLRWLDRPVPDLEHARTGLRDIIAEGQRAGGIISGLRDLT 366 Query: 332 RQVDHAAAIIKSVREYVVKLEQSEGLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEAL 391 R +AA + + V + V + +L R +E R V L + + Sbjct: 367 R---NAAPVCERVNLHAV----------IRHILTLSRSELERR----EVSLLLSLTPADA 409 Query: 392 VVSCEKVLIGQVILNLAFNAIEEMSHFPQSQRRVCISLARKDQQAL-LTVADEGRGIDAG 450 V ++V + QV+LNL NAIE MS P+ R + I+ + D+Q + + V D G GI Sbjct: 410 FVLGDQVQLQQVMLNLVVNAIEAMSSMPEHARVLSITASSCDEQRIEVKVQDSGEGIAPE 469 Query: 451 HRERLFDGFFSSKVSGNGIGLALCKNIIARHRGDIWAQPAQVRGTRFCFTLPLV 504 R+FD F+++K SG G+GLA+C++II HRG + AQ G F F LP V Sbjct: 470 AMRRVFDAFYTTKDSGMGMGLAICRSIIENHRGRLRVAAAQPHGALFSFDLPRV 523 >gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 600 Score = 82.8 bits (203), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 70/253 (27%), Positives = 118/253 (46%), Gaps = 20/253 (7%) Query: 260 EAEEARRRDQAALEYLARHNAMGEMATAIAHELSQPLAAIRNFLEGSVLRLNHSEALREQ 319 + E R Q L + +G+MA + HEL+QPL AIR F + +V L R Q Sbjct: 358 QTESLLRTTQNELVQAGKLAMLGQMAAGVTHELNQPLTAIRAFADNAVKFLA-----RGQ 412 Query: 320 AGQIIWGLESAGRQVDHAAAIIKSVREYVVKLEQSEGLIDLNEVLAEVRWFI--EMRAAE 377 + Q + LE II ++ + K ++ +DL++ + + E + Sbjct: 413 SAQAVENLEYISSASATMGKIIAQLKGFARKSGEAVACVDLSQAIEASSLLLASECQKLG 472 Query: 378 GRVQLEVQASEEALVVSCEKVLIGQVILNL---AFNAIEEMSHFPQSQRRVCISLARKDQ 434 + ++++A A V+ + V QV++NL A +A+EE H +R+ + L Sbjct: 473 ATISIDIRA---AACVTGDVVRTEQVLVNLLRNALDAVEEAEH-----KRIAVVLEVAGG 524 Query: 435 QALLTVADEGRGIDAGHRERLFDGFFSSKVS--GNGIGLALCKNIIARHRGDIWAQPAQV 492 AL+ + D G GI LF+ FF++K S G G+GLA+ +I+ G + A + Sbjct: 525 YALVRIRDSGGGIADEVAPHLFEPFFTTKSSSKGLGLGLAISSSIVQAMNGQLSAHNHEE 584 Query: 493 RGTRFCFTLPLVQ 505 G F LPL++ Sbjct: 585 GGAEFVVRLPLLK 597 >gi|300309416|ref|YP_003773508.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 760 Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 100/407 (24%), Positives = 172/407 (42%), Gaps = 62/407 (15%) Query: 134 EQIRREMKKLESRLKEFMQDSDEGVAVLRPDGGIEYLS--DAGRKLLG--VRPGQNPPKN 189 E+ R E++ ++ L+ + + GV VL DG LS D+ R++LG P Sbjct: 370 EKNRAELENAKAYLESVLANMSAGVMVL--DGQFNILSANDSVRRILGHDFSADIGTPLQ 427 Query: 190 FVAQCLPDSQ-------EELQELLTLALPDAISFPLRYRIARKDGLV---------RWHH 233 + P +Q E+L + + + PD + + ++ ++R+ G + + H Sbjct: 428 TIEAQAPFAQAVIRAFSEQLAQHSSDSTPDGLHWQRQFELSRQPGAMLDSDDEQGGKPDH 487 Query: 234 ------AVCRYIEIENDLKGHLLHFHDITDQVEAEEARRRDQAALEYLARHNAMGEMATA 287 A ++ +EN + G+++ F DI++ + A+ R A GE+A Sbjct: 488 DKLTLLARGSHLPVENGV-GYVVVFDDISNVISAQ--------------RSIAWGEVARR 532 Query: 288 IAHELSQPLAAIRNFLEGSVLRLNH--SEALREQAGQII-WGLESAGRQVDHAAAIIKSV 344 +AHE+ PL I+ E RL H S+ L I+ G + QV ++ Sbjct: 533 LAHEIKNPLTPIQLSAE----RLQHRLSDKLMPADAAILDKGTTTIVNQVTAMKRMVDDF 588 Query: 345 REYVVKLEQSEGLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEALVVSCEKVLIGQVI 404 R+Y +DLN ++ E+ A +GR + + + V + + QVI Sbjct: 589 RDYARTPPAKPVALDLNALIEEILNL--YLAGDGRDAIHARLAPSMAKVMGDATQLRQVI 646 Query: 405 LNLAFNAIEEMSHFPQSQRRV-----CISLARKDQQ----ALLTVADEGRGIDAGHRERL 455 NL NA + ++ + R+ I +A D Q L++ D G G A R Sbjct: 647 HNLLQNAQDAVADEGEVAPRIDVLTEQIDIAGADGQRQPAVRLSIMDNGPGFSAKILARA 706 Query: 456 FDGFFSSKVSGNGIGLALCKNIIARHRGDIWAQP-AQVRGTRFCFTL 501 F+ + +SK G G+GLA+ K I+ H G I Q RG + L Sbjct: 707 FEPYVTSKSKGTGLGLAMVKKIVEEHGGRIDIQNRTDGRGAKIVILL 753 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,542,749 Number of extensions: 61406 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 306 Number of HSP's successfully gapped: 18 Length of query: 508 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 419 Effective length of database: 1,198,455 Effective search space: 502152645 Effective search space used: 502152645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)