BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0181.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310681|ref|YP_003774773.1| amino acid ABC transporter peri... 769 0.0 gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC t... 340 1e-94 gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC t... 284 8e-78 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 84 1e-17 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 75 7e-15 >gi|300310681|ref|YP_003774773.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 769 bits (1985), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/385 (98%), Positives = 381/385 (98%) Query: 1 MLHKRLIAAAVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL 60 MLHKRLIAA VLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL Sbjct: 1 MLHKRLIAAVVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL 60 Query: 61 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAPVG 120 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAPVG Sbjct: 61 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAPVG 120 Query: 121 TAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTAAFITVNDPFGENWARVFSD 180 TAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTA FI VNDPFGENWARVFSD Sbjct: 121 TAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTAGFIAVNDPFGENWARVFSD 180 Query: 181 LAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGYKG 240 LAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGYKG Sbjct: 181 LAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGYKG 240 Query: 241 KIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVAMDYIEAYKKMYNA 300 KIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVA DYIEAYKKMYNA Sbjct: 241 KIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVATDYIEAYKKMYNA 300 Query: 301 VPATFGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVYNMSAT 360 VPATFGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVYNMSAT Sbjct: 301 VPATFGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVYNMSAT 360 Query: 361 DHSGFDDRGRELITVKGGNWALLKQ 385 DHSGFDDRGRELITVKGGNWALLKQ Sbjct: 361 DHSGFDDRGRELITVKGGNWALLKQ 385 >gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 340 bits (872), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 170/357 (47%), Positives = 237/357 (66%), Gaps = 1/357 (0%) Query: 25 AQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDLKVEYISLDDASDPTKSVTEIKKLI 84 A + VGV S+TGP + +GIP+KNT+ LLPT I KV YI LDDASD T++V ++ + Sbjct: 21 ADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNYIILDDASDTTQAVKNARRFV 80 Query: 85 AEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAPVGTAAVVLPMTEQKKWVFKTTQNDDL 144 +E+NVD I+G S SPN++ +I A+A P+++ A +V PM +KKWVFKT QND + Sbjct: 81 SENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARIVEPMDAKKKWVFKTPQNDIM 140 Query: 145 IAKALVDQMVKTKVKTAAFITVNDPFGENWARVFSDLAAKNNIKIVANERYQRTDTSVTA 204 ++ A+ MV + AFI ++ +GE W F A +++VANER+ TD+S TA Sbjct: 141 MSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGLKKLELVANERFAPTDSSTTA 200 Query: 205 QSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGYKGKIYQTHGAALPAFLQLGKNKVEGT 264 Q+LK+VAA PDA+L+AA G V+PQ L ++G+KG+IYQTHG A FL++G ++GT Sbjct: 201 QALKLVAAKPDAILIAASGTPAVVPQRALKERGFKGQIYQTHGVANADFLRVGGKMLDGT 260 Query: 265 ILAASLMLVLPEIPDSNPSKKVAMDYIEAYKKMYNAVPAT-FGANVYDAGLLLEKAIPVA 323 +L A +LV ++P SNP +KVA+DY Y+ ++ + FG +DAG+LL AIPVA Sbjct: 261 LLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGKDSVSLFGGYAWDAGVLLRWAIPVA 320 Query: 324 ARKAKPGSKEFRAALRDALENTKEVVGTQGVYNMSATDHSGFDDRGRELITVKGGNW 380 + AKPG+ EFRAALRD LE TKEV G GVY MS+TDH G D R ++ + G W Sbjct: 321 LKGAKPGTPEFRAALRDVLEATKEVTGVHGVYTMSSTDHLGLDQRAAVMVKIVDGAW 377 >gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 284 bits (727), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 161/383 (42%), Positives = 226/383 (59%), Gaps = 10/383 (2%) Query: 1 MLHKRLIAAAVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL 60 M K I A VL M + A A A++ +GVV S TGP S +GIP KN L P I Sbjct: 1 MKTKLAIKAGVLAAMLACA-GIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGE 59 Query: 61 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLA--P 118 KV+ I LDDA+DPT++ ++L+AE VD IIG + P + + E LA P Sbjct: 60 KVKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISP 119 Query: 119 VGTAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTAAFITVNDPFGENWARVF 178 + TA + W F+ Q+ ++A +V+QM K VKT F+ +D +GENW + Sbjct: 120 IETA------EGKDAWTFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEV 173 Query: 179 SDLAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGY 238 LA +K+ ER+ R DTSVTAQ+L++V++NPDAVLV A G+ +P L ++GY Sbjct: 174 QRLATAAGMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIERGY 233 Query: 239 KGKIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVAMDYIEAYKKMY 298 KGKI+QTH AA ++LG VEG + + +V +PDSN SKK+AMD++ Y+K + Sbjct: 234 KGKIFQTHSAASRDLIRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQF 293 Query: 299 NAVPAT-FGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVYNM 357 A F A+ YDA L+L+K +P A +KAKPG+ FRAAL++ALE + + TQG N Sbjct: 294 GAGSRNLFAAHTYDAQLVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTLNY 353 Query: 358 SATDHSGFDDRGRELITVKGGNW 380 S DH G D R ++T++ GNW Sbjct: 354 SPKDHFGLGDDARIMLTIQNGNW 376 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 106/395 (26%), Positives = 168/395 (42%), Gaps = 41/395 (10%) Query: 10 AVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKI-------GDLKV 62 A+L+G ++L+ A +K+GV + TGP + G+ KN L +I GD K+ Sbjct: 12 ALLIG-ATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGD-KL 69 Query: 63 EYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAPVGTA 122 + D+ +++ KKLI + V A+ GP+ S +A A V + GT+ Sbjct: 70 ALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVF---GTS 126 Query: 123 AVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKT-KVKTAAFITVND-PFGENWARVFSD 180 +T + F+ + + + V VK +K A I ND F ++ VF Sbjct: 127 NTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKA 186 Query: 181 LAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVA--APGAATVLPQTTLFDQGY 238 I + E Y + D AQ KI A NPDA++ + A AA ++ QT G Sbjct: 187 TLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRAL--GM 244 Query: 239 KGKIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVAMDYIEAYKKMY 298 K +G P ++ K+ + T++ + + P+ K +I AYK + Sbjct: 245 KQPFIGGNGLNSPKLFEIAKDAGDNTLMGSPW-----SAENQTPANKA---FITAYKAKF 296 Query: 299 NAVPATFGANVYDAGLLLEKA---IPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVY 355 A P F A YDA ++ A + ++ AK R ALR AL K + G G + Sbjct: 297 GADPDQFAAQAYDAMYIVADALKNVKLSGNLAKD-----RDALRAALPAVK-IDGATGKF 350 Query: 356 NMSAT-----DHSGFDDRGRELITV-KGGNWALLK 384 A GFD ++ + KGG + LLK Sbjct: 351 AFRAAPAVAGKQVGFDADQEAIVNIAKGGKFVLLK 385 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 75.5 bits (184), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 96/352 (27%), Positives = 155/352 (44%), Gaps = 32/352 (9%) Query: 6 LIAAAVL-LGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKI---GDL- 60 +AAA++ L ++S A P +K+GV TG + +G+ ++ V L +I G L Sbjct: 8 FVAAALMSLALTSQAADP----IKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLL 63 Query: 61 --KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAP 118 +++ I DD + + V ++LI + V A +G + A+ F EA +P++ Sbjct: 64 GRQIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINN 123 Query: 119 VGTAAVVLPMTEQKK--WVFKTTQNDDLIAKALVDQMV-KTKVKTAAFITVNDPFGENWA 175 V T V+ E++K +VF+T+ D + AK + D+ V K K A + + +G+ Sbjct: 124 VATGTVITKQFEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAILADSTNYGQLGR 183 Query: 176 RVFSDLAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFD 235 + A + VA E+Y D +TAQ LK AVL G + Sbjct: 184 EDLEKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQIANGMEK 243 Query: 236 QGYKGKIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVAMDYIEAYK 295 + I + ++ F+ G + + I D N K+ A +IEAY+ Sbjct: 244 LNWHVPIIGSWPLSMGNFIDNSGKNGNGARMPQTF------IQDGNTPKRKA--FIEAYQ 295 Query: 296 KMY--NAVP-ATFGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALEN 344 K Y + +P A A YD+ LLL AI A G K +R ALEN Sbjct: 296 KAYKVDRMPSAVSAAQGYDSILLLAAAIKQAG--TTDGDK-----VRAALEN 340 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,141,161 Number of extensions: 44629 Number of successful extensions: 203 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 11 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)