BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0180.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310682|ref|YP_003774774.1| amino acid ABC transporter perm... 670 0.0 gi|300313406|ref|YP_003777498.1| branched-chain amino acid ABC t... 312 2e-86 gi|300313299|ref|YP_003777391.1| branched-chain amino acid ABC t... 281 6e-77 gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC tr... 71 1e-13 gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC t... 67 3e-12 >gi|300310682|ref|YP_003774774.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 344 Score = 670 bits (1728), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/344 (100%), Positives = 344/344 (100%) Query: 1 MNLQIVALLGQDGITNGAIYALLALSIVLVFTVTRILLIPQGEFVSFGALTMGMMQNGQP 60 MNLQIVALLGQDGITNGAIYALLALSIVLVFTVTRILLIPQGEFVSFGALTMGMMQNGQP Sbjct: 1 MNLQIVALLGQDGITNGAIYALLALSIVLVFTVTRILLIPQGEFVSFGALTMGMMQNGQP 60 Query: 61 LTVAWLLVAMCLGACAFDLYDMWRTSRRRPSLWLPAKLAYAAVVGGVALSVNFATLPMLV 120 LTVAWLLVAMCLGACAFDLYDMWRTSRRRPSLWLPAKLAYAAVVGGVALSVNFATLPMLV Sbjct: 61 LTVAWLLVAMCLGACAFDLYDMWRTSRRRPSLWLPAKLAYAAVVGGVALSVNFATLPMLV 120 Query: 121 QIAMTLLLLVPLGPLMYRMAFQPIATASPLVLLIVSIAVHVALVGVALLVFGPEGARTAP 180 QIAMTLLLLVPLGPLMYRMAFQPIATASPLVLLIVSIAVHVALVGVALLVFGPEGARTAP Sbjct: 121 QIAMTLLLLVPLGPLMYRMAFQPIATASPLVLLIVSIAVHVALVGVALLVFGPEGARTAP 180 Query: 181 FTEAALELGSLRLSSQALWVICVSLLLIVGLFLMFERTLYGKALRAAALNRMGARLMGIS 240 FTEAALELGSLRLSSQALWVICVSLLLIVGLFLMFERTLYGKALRAAALNRMGARLMGIS Sbjct: 181 FTEAALELGSLRLSSQALWVICVSLLLIVGLFLMFERTLYGKALRAAALNRMGARLMGIS 240 Query: 241 PVFAGKAMFLLASLIGVMSGVLIAPITTIYYDSGFLISLKGFVGSIIGGLVSYPLAAAGA 300 PVFAGKAMFLLASLIGVMSGVLIAPITTIYYDSGFLISLKGFVGSIIGGLVSYPLAAAGA Sbjct: 241 PVFAGKAMFLLASLIGVMSGVLIAPITTIYYDSGFLISLKGFVGSIIGGLVSYPLAAAGA 300 Query: 301 LAIGLIESFSTFWASAYKEIIVFVLIIPILLWRSLTTRHVEEEE 344 LAIGLIESFSTFWASAYKEIIVFVLIIPILLWRSLTTRHVEEEE Sbjct: 301 LAIGLIESFSTFWASAYKEIIVFVLIIPILLWRSLTTRHVEEEE 344 >gi|300313406|ref|YP_003777498.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 350 Score = 312 bits (800), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 182/349 (52%), Positives = 250/349 (71%), Gaps = 6/349 (1%) Query: 1 MNLQIVALLGQDGITNGAIYALLALSIVLVFTVTRILLIPQGEFVSFGALTMGMMQNGQP 60 M+ I+ +L QDGIT GAIYALL+L++VLVF+VTR++ +PQGEFV+FGALT+ +QNGQ Sbjct: 1 MDTSILTVLIQDGITTGAIYALLSLALVLVFSVTRVIFLPQGEFVAFGALTLAAIQNGQA 60 Query: 61 LTVAWLLVAMCLGACAFDLYDMWRTSRRRPSLWLPAKLAYAAVVG------GVALSVNFA 114 A LL+A+ A + R ++ R L L A +G + ++ Sbjct: 61 PATALLLLALGAVTLAVETGSALRLAQTRARLLRRLPLLLATHLGLPLAILALTRCLDLQ 120 Query: 115 TLPMLVQIAMTLLLLVPLGPLMYRMAFQPIATASPLVLLIVSIAVHVALVGVALLVFGPE 174 L M QI + LL++VP+GP++YR+A+QP+A +S LVLLIVS+ VH+A+VG+ LL+FGPE Sbjct: 121 ALAMPWQILLALLIVVPMGPMIYRVAYQPLAHSSVLVLLIVSLGVHLAMVGLGLLMFGPE 180 Query: 175 GARTAPFTEAALELGSLRLSSQALWVICVSLLLIVGLFLMFERTLYGKALRAAALNRMGA 234 G T PFTE LG +S Q++ V+ LI +++ FERTL GKALRA A+NR+GA Sbjct: 181 GTNTRPFTEMQFNLGESPVSGQSVIVLITVAALIAVIYVFFERTLTGKALRATAVNRLGA 240 Query: 235 RLMGISPVFAGKAMFLLASLIGVMSGVLIAPITTIYYDSGFLISLKGFVGSIIGGLVSYP 294 RL+G+ AGK F +A+ IG + G+LIAP T + Y+SGF+I+LKGFVG+I GGL SYP Sbjct: 241 RLVGVGINQAGKLSFGMAAGIGALCGILIAPFTIVNYESGFIIALKGFVGAIFGGLASYP 300 Query: 295 LAAAGALAIGLIESFSTFWASAYKEIIVFVLIIPILLWRSLTTRHVEEE 343 +AA G++++GLIESFS+FWASAYKE+IVF +IIPILLWRSLT+ H++EE Sbjct: 301 IAALGSVSVGLIESFSSFWASAYKELIVFTVIIPILLWRSLTSHHIDEE 349 >gi|300313299|ref|YP_003777391.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 346 Score = 281 bits (719), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 181/350 (51%), Positives = 239/350 (68%), Gaps = 10/350 (2%) Query: 1 MNLQIVALLGQDGITNGAIYALLALSIVLVFTVTRILLIPQGEFVSFGALTMGMMQNGQ- 59 M+ I A+L DG+TNG +Y L+A+++VLVFTVTRIL IPQGEFV++GALT+ M+Q GQ Sbjct: 1 MDSSIAAILTLDGLTNGIVYGLIAIALVLVFTVTRILFIPQGEFVAYGALTLAMLQQGQR 60 Query: 60 --PLTVAWLLVAMCLGACAFDLYDMWRTSRRRPSLW--LPAKLAYAAVVGGVALSVNFAT 115 PL + +L G A L + + +L L L AA+V A FA Sbjct: 61 PGPLWLLLVLALCAAGMQAASLLRAGQAALLPRALLGTLGVPLVIAALVWWAA-PQQFA- 118 Query: 116 LPMLVQIAMTLLLLVPLGPLMYRMAFQPIATASPLVLLIVSIAVHVALVGVALLVFGPEG 175 LP VQ+ +TLLL+ LGPLMY++ ++ +A AS L LLIVS+ +H+A+ G+ L+ FG EG Sbjct: 119 LP--VQVLLTLLLITALGPLMYQVVYESMADASVLQLLIVSVGIHLAMTGLGLVFFGAEG 176 Query: 176 ARTAPFTEAALELGSLRLSSQALWVICVSLLLIVGLFLMFERTLYGKALRAAALNRMGAR 235 RT F + L SL LS+Q + V+ + L+V L+ RTLYGKALRA A+NR+GAR Sbjct: 177 FRTPAFWDERWNLASLTLSAQTVVVVAAAAALLVALWFFSGRTLYGKALRATAVNRLGAR 236 Query: 236 LMGISPVFAGKAMFLLASLIGVMSGVLIAPITTIYYDSGFLISLKGFVGSIIGGLVSYPL 295 LMGIS AGK F +A+ IG +SGVLI P++TI+YDSGFLI LKGFV + IG L S+P Sbjct: 237 LMGISTTLAGKLSFAVAAFIGALSGVLIGPMSTIFYDSGFLIGLKGFVAATIGALASFPA 296 Query: 296 AAAGALAIGLIESFSTFWASAYKEIIVFVLIIPILLWRSLT-TRHVEEEE 344 AA GAL +GL+ESFS+FWAS +KE+IVF+ ++PILLWRSL H E+EE Sbjct: 297 AAGGALLVGLLESFSSFWASDFKEVIVFLALLPILLWRSLVIPAHDEDEE 346 >gi|300310992|ref|YP_003775084.1| branched-chain aminoacid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 308 Score = 70.9 bits (172), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 62/203 (30%), Positives = 106/203 (52%), Gaps = 7/203 (3%) Query: 138 RMAFQPIATASPLVLLIVSIAVHVALVGVALLVFGPEGARTAPFTEAALE--LGS---LR 192 R+A++P+ LV LI +I + + L + G ALE +GS + Sbjct: 93 RVAYRPLRGGPRLVPLISAIGMSIFLQNYVQIGQGARDMSVPVLISGALEFQMGSDFTVT 152 Query: 193 LSSQALWVICVSLLLIVGLFLMFERTLYGKALRAAALNRMGARLMGISPVFAGKAMFLLA 252 + + ++ V+L+L+V L L R+ G+A RA A + A L+GI F+L Sbjct: 153 IPYSRMVIVGVTLVLMVALTLFIARSRMGRACRACAEDMGMANLLGIDTNKVISFTFVLG 212 Query: 253 SLIGVMSGVLIA-PITTIYYDSGFLISLKGFVGSIIGGLVSYPLAAAGALAIGLIESFST 311 +++ + GVLIA I + GF+ +K F +++GG+ S P A G + +GL E+F+ Sbjct: 213 AMLAAVGGVLIALTIGKLNPYIGFIAGIKAFTAAVLGGIGSIPGAMLGGVLLGLAETFAA 272 Query: 312 -FWASAYKEIIVFVLIIPILLWR 333 + S YK+++ F L++ ILL+R Sbjct: 273 GYLPSEYKDVVSFGLLVLILLFR 295 >gi|300310521|ref|YP_003774613.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 309 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 65/236 (27%), Positives = 122/236 (51%), Gaps = 21/236 (8%) Query: 116 LPMLVQIAMTLLLLVPL----GPLMYRMAFQPIATASPLVLLIVSIAVHVALVGVALLVF 171 LP +VQ+ + ++ +P+ L+ R+A++P+ A L LI +I V + L +A++++ Sbjct: 64 LPGIVQLVIAIVGAIPVCIVVSLLIERIAYRPLRNAPRLAPLITAIGVSILLQTLAMMIW 123 Query: 172 GPEGARTAPFTEA----ALELGSLRLSSQALWVICVSLLLIVGLFLMFERTLYGKALRAA 227 G PF + + + +S + ++ +++L +VGL L+ E+T G+A+RA Sbjct: 124 GRS---PLPFPQVMPSDPVHIAGALISPTQIMLLALAVLAMVGLVLIVEKTKMGRAMRAT 180 Query: 228 ALNRMGARLMGISPVFAGKAMFLLASLIGVMSGVL-IAPITTIYYDSGFLISLKGFVGSI 286 A N A LMG+ F + + + ++GV+ A +T + GF+ LK F ++ Sbjct: 181 AENPRIAGLMGVDANKVIVVTFAIGAGLAAIAGVMWAANYSTAQFAMGFVPGLKAFSAAV 240 Query: 287 IGGLVSYPLAAAGALAIGLIESF---------STFWASAYKEIIVFVLIIPILLWR 333 +GG+ + A G + +GLIES F S Y++I F+++I +L R Sbjct: 241 LGGIGNIYGAMLGGILLGLIESLGAGYIGDLTGNFLGSNYQDIFAFIVLIIVLTLR 296 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 846,141 Number of extensions: 30382 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 238 Number of HSP's successfully gapped: 12 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)