BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0174.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 795 0.0 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 386 e-108 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 344 8e-96 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 136 3e-33 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 124 1e-29 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 795 bits (2052), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/400 (99%), Positives = 399/400 (99%) Query: 1 MRTPPIPKPVAALTALAILSVLSGCGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGR 60 MRTPPIPKPVAALTALAILSVLS CGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGR Sbjct: 1 MRTPPIPKPVAALTALAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGR 60 Query: 61 TSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASV 120 TSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASV Sbjct: 61 TSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASV 120 Query: 121 RTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAP 180 RTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAP Sbjct: 121 RTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAP 180 Query: 181 ISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG 240 ISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG Sbjct: 181 ISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG 240 Query: 241 NGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQE 300 NGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQE Sbjct: 241 NGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQE 300 Query: 301 GVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQL 360 GVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQL Sbjct: 301 GVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQL 360 Query: 361 VVDGQQRASAGAKVKVVPWTPKASLASVANTAASAAAGAH 400 VVDGQQRASAGAKVKVVPWTPKASLASVANTAASAAAGAH Sbjct: 361 VVDGQQRASAGAKVKVVPWTPKASLASVANTAASAAAGAH 400 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 386 bits (991), Expect = e-108, Method: Compositional matrix adjust. Identities = 203/358 (56%), Positives = 261/358 (72%), Gaps = 2/358 (0%) Query: 15 ALAILSVLSGCGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVN 74 +L +++VL+ C + G P +G V V AQ L TELPGRT+ Y AD+RPQV Sbjct: 24 SLILVAVLAACSRS-GDQPKPTAQAEVGYVVVKAQRQALTTELPGRTTAYLAADIRPQVG 82 Query: 75 GIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELV 134 GII+ R F+EGS VKAGQ LYQI+PASYQAAYDS+ A+L KAEA++ +++LKA RY EL Sbjct: 83 GIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERYAELN 142 Query: 135 AIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGA 194 I AVS+QD DDA ++L + +ADV +A VQT+ INL Y ++ +PISGRI KS+VTPGA Sbjct: 143 KIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSAVTPGA 202 Query: 195 LVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLENGR 253 LVTA+QTTALTT+QQ+DPIYVDVTQS+ L L+ LA+GQ+ KSG N A+V++VLE+G Sbjct: 203 LVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQVKIVLEDGS 262 Query: 254 TYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQA 313 TY + G L+F+ ++V+ TG ITLRA+ PNP LLPG YVRAVL+EGV LL PQ+A Sbjct: 263 TYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLAPQKA 322 Query: 314 VSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAG 371 VSRD G A V+ A G V+QR + RA+G+ W VS GLKEGD+L+VDG Q+ G Sbjct: 323 VSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDGLQKVRTG 380 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 344 bits (882), Expect = 8e-96, Method: Compositional matrix adjust. Identities = 190/390 (48%), Positives = 260/390 (66%), Gaps = 10/390 (2%) Query: 16 LAILSVLSGCGKPP----GGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRP 71 +AI + L CG+ P G PP+ + VVT+ Q V + ELPGR +++A VR Sbjct: 14 VAIAAALVACGQKPAAQGGPMPPSE----VSVVTIAPQRVAITNELPGRVEAFRVAQVRA 69 Query: 72 QVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYK 131 +V GI++ R F+EGS+VK GQVL++I+PA Y+A+ +S+ A LA+A+A++ L A RYK Sbjct: 70 RVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYK 129 Query: 132 ELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVT 191 LV + AVS+Q+YD+AVA+ +AEADV + +A VQT+ +NL YA + +PISGRIG++ VT Sbjct: 130 PLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVT 189 Query: 192 PGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLE 250 GALV T L +QQLDP+YV+VTQSS L L++A+A GQL+ G N A V L LE Sbjct: 190 EGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELE 249 Query: 251 NGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVP 310 NG+ Y G L FSD++V+++TG+++LRA FPNPK LLPG YVRA +++ V +Q LLVP Sbjct: 250 NGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVP 309 Query: 311 QQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASA 370 QQAV RD G V VVNA+ VE R + T A G+ W V GLKEGD+++V+G Q+ Sbjct: 310 QQAVVRDAGGSTV-MVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRP 368 Query: 371 GAKVKVVPWTPKASLASVANTAASAAAGAH 400 GA VK V W P + A + A A A Sbjct: 369 GATVKPVQWNPAGAPAQASGQPAKQEASAQ 398 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 107/337 (31%), Positives = 160/337 (47%), Gaps = 7/337 (2%) Query: 41 LGVVTVHAQPVELHTELPGRTSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPA 100 +GV Q V + G +P A VR +V+G + ++EG VKAG +L +I+P Sbjct: 34 VGVAKARLQDVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPR 93 Query: 101 SYQAAYDSSVAALAKAEASVRTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGS 160 S QAA + LA+ A + +ARL RY+ L+A +++ Q D VA + + E V + Sbjct: 94 SLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKA 153 Query: 161 ARANVQTSRINLDYARLDAPISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQS 220 NV ++R+ L + R+ APISGR+G G VT + T L I QL PI Sbjct: 154 DEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIF--- 210 Query: 221 STTWLNLKEALANGQLEKSGNGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAI 280 S N+ + L Q +SG + + +G L D V+ TG + L+A Sbjct: 211 SIPEDNIPKVL---QQLQSGRKLPAQAWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQ 267 Query: 281 FPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVT 340 FPN L P +V LQ Q ++P A+ R G V +VV V R + T Sbjct: 268 FPNTDYALFPSQFVNIRLQLNTEQGATVIPTAAIQRGSKGLFV-YVVKDDSSVTVRPVKT 326 Query: 341 DRAIGDQWLVSSGLKEGDQLVVDGQQRASAGAKVKVV 377 D +++ G+ G+ +V+DG R GAKV+ V Sbjct: 327 GPVQDDLTVITDGVSAGETVVIDGIDRLREGAKVEAV 363 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 94/310 (30%), Positives = 153/310 (49%), Gaps = 12/310 (3%) Query: 69 VRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKAS 128 V+ +++G ++ F+EG DVK GQ+L QI+ + QA + A AK A++ ARL Sbjct: 79 VKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQ 138 Query: 129 RYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKS 188 RY L + A +QQ D A + + EA V + A V +++ L Y ++APISGR+G Sbjct: 139 RYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGAR 198 Query: 189 SVTPGALVTANQTTALTTIQQLDPIYVDVT--QSSTTWLNLKEALANGQLEKSGNGAKVR 246 V PG +V A L I Q+DPI + T + + +N + L+ S + Sbjct: 199 LVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQ 258 Query: 247 LVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQG 306 VL G+ L D +N G + L+A F NP L PG YV A LQ Sbjct: 259 EVLATGK-------LVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNA 311 Query: 307 LLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSG--LKEGDQLVVDG 364 L VP A+ R+ G V + ++ + + + +R ++++G ++ G ++V+DG Sbjct: 312 LTVPAAAIQRNQQGTYV-YAIDQEDQARMQPVKVERIQDGIAVLAAGNAVQAGQRVVIDG 370 Query: 365 QQRASAGAKV 374 Q + G+++ Sbjct: 371 QYKLKPGSRI 380 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.128 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,094,342 Number of extensions: 43295 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 12 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)