BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0167.p (578 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 1107 0.0 gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 105 7e-24 gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herba... 77 5e-15 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 69 1e-12 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 1107 bits (2864), Expect = 0.0, Method: Compositional matrix adjust. Identities = 554/555 (99%), Positives = 555/555 (100%) Query: 24 MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA 83 MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA Sbjct: 1 MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA 60 Query: 84 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 143 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL Sbjct: 61 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 120 Query: 144 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 203 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP Sbjct: 121 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 180 Query: 204 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK 263 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK Sbjct: 181 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK 240 Query: 264 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV 323 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV Sbjct: 241 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV 300 Query: 324 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQAVLPVHISQPFAYAMVGMGAFLAAA 383 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQA+LPVHISQPFAYAMVGMGAFLAAA Sbjct: 301 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHISQPFAYAMVGMGAFLAAA 360 Query: 384 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 443 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL Sbjct: 361 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 420 Query: 444 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL 503 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL Sbjct: 421 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL 480 Query: 504 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK 563 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK Sbjct: 481 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK 540 Query: 564 TSLLDAYFRLDRAGN 578 TSLLDAYFRLDRAGN Sbjct: 541 TSLLDAYFRLDRAGN 555 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 105 bits (263), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 145/511 (28%), Positives = 229/511 (44%), Gaps = 38/511 (7%) Query: 74 LLPTCGGVVAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSLASIASGG 132 LLP GG++ GL + G+G + +EA+ G + + LL+ +SS I SGG Sbjct: 68 LLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSGIVIGSGG 127 Query: 133 SIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIV 192 G EGP++ SL+ + H ++ + L+ GA AG+T+ + P+A E++ Sbjct: 128 PFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAVLLAVELL 187 Query: 193 LGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFP-EIGGWE---VMLFLVLGVLA 248 L L S PV +A VA L P FP ++ ++ LV G+ + Sbjct: 188 LFELKPRSLLPVALACAVAGFVRPWL-----VEAGPLFPLDVAAPTPVALLSCLVAGLAS 242 Query: 249 GVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHT 308 GVLA L + GF +LRL +GG+ VGL + P G GY ++ LLH Sbjct: 243 GVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLLHQ 302 Query: 309 SWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQAVLPVHISQP 368 + A L +L+VK + + +GSG GG+ P L +GA +G LP Sbjct: 303 HMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGL----VLSPWLPG--GSD 356 Query: 369 FAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAY-----VVARSVDG 423 + +V M L + APL AI+ F +T + Q +LPL+ + VAY ++ RS+ Sbjct: 357 GLWPLVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMT 416 Query: 424 RSMYEITLKRHR----DNEERLRLRGTHMSELIRPA-ETVLHEEASLEEISALFLKYPVK 478 + L HR D ER+ H+ EL+ A +T+ + E ++ + V Sbjct: 417 EKIARRGLHVHREYGVDPLERM-----HVEELMTAAPQTLAGALPAAEALAWCRAQEHVH 471 Query: 479 YVYIVDGRQRYLGVVALQDITSWLLDKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLF 538 Y V +G++AL+ + + A D L H + P MS A + Sbjct: 472 RYYPVTDGVHLVGMLALRAL-------EHADASQPCDTLAQGHEHYLLPHMSARSAAGMM 524 Query: 539 LDHQGERLPVIASADDPQLLGVVFKTSLLDA 569 RLPV+A + +L+GV+ + L A Sbjct: 525 ARLGVARLPVVAGPERRKLVGVLSLSDLARA 555 >gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 76.6 bits (187), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 129/453 (28%), Positives = 198/453 (43%), Gaps = 50/453 (11%) Query: 11 RLLNACRVSESHSMLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWY 70 R L+ ++S+SH LW G + + A I + Q G G F I PW Sbjct: 13 RQLSLYQLSKSHLRRLW-VEYGILWSGAVIVGLVAVLYAQAIEIGF-GVFRSIQHRSPWL 70 Query: 71 TRVLLPT-CGGVVAGLFLVWAKRT--PAGNGGDYMEAVAIGDGAIPVRNTLLRSIS---- 123 +L P C V W R P G +A+A G R + L S+ Sbjct: 71 PLLLTPAVCAACV------WLTRRYFPGSEGSGIPQAIATLHGDTAERGSTLLSLRVLLG 124 Query: 124 ----SLASIASGGSIGREGPMVQL-SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAY 178 S+ +IA G +IGREGP VQ+ +A+ +L + R L+ GAAAG+ +A+ Sbjct: 125 KIVLSVVAIAGGMTIGREGPTVQIGAAIMYNLRRLYPAGYEHMERRLILAGAAAGLAAAF 184 Query: 179 NAPIAGAFFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFP-EIGGWE 237 N P+AG F E + S + G V+ + A + + G + FP E G Sbjct: 185 NTPLAGIVFAIEELSRSFEQRASGVVITTIIFAGLVALSINGNYTYFGSIAFPGETSGRL 244 Query: 238 VMLFLVLGVLAGVLAPQF----LRVLELGKHGFGKLRLPLPVRL-GVGGLLVGLISVQV- 291 ++ +V VL GV F L LR PV V G +V +I V Sbjct: 245 ILAVIVTAVLMGVAGGLFCWLILNPTRWIPPALVTLRTERPVAFAAVCGFIVAVIGVAAG 304 Query: 292 PEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKVLATSITVGS---GAVGGIFTPTLFVGAA 348 +G+GY + LL+ Q + + L + +GS G GGIF P+L +GA Sbjct: 305 GATFGSGYEQAHGLLNDG---QG--LSVFYPFLKFASMIGSYLPGLPGGIFAPSLSIGAG 359 Query: 349 VG----YLFGDAAQAVLPVHISQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVV 404 G LFG + LP+ + A AMVG +LAA + +P+ A +++ EM + +V Sbjct: 360 FGNLLHLLFGGTS---LPMLV----ALAMVG---YLAAVTQSPITAFVIVMEMVDGHALV 409 Query: 405 LPLMLSCVVAYVVARSVDGRSMYEITLKRHRDN 437 + LM + ++A V++ V +YE +R+ + Sbjct: 410 ISLMATALIASAVSK-VFAPPLYETLAERYLKS 441 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 68.9 bits (167), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 97/359 (27%), Positives = 152/359 (42%), Gaps = 38/359 (10%) Query: 74 LLPTCGGVVAGLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGS 133 LLP G V L+L K AGN ++ + IP+R L + ++AS G S Sbjct: 57 LLPLGGFAVGWLYLHTGKPVEAGNN-LLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGAS 115 Query: 134 IGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIV- 192 +GREG VQ+ A + R+L+ G +AG +S + P+AGA F E++ Sbjct: 116 VGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLA 175 Query: 193 LGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGV---LAG 249 +G + E+ P VA++ A+ + Y M + W V + GV LAG Sbjct: 176 IGRMRYEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGLAG 235 Query: 250 VLAPQFLRVLELGKHGFGKLRLPL-PVRLGVGGLLVGLISVQVPEVWGNGYS-------- 300 ++ L F K + P+R +GG +V L VW G Sbjct: 236 MIFANSTHTLS----AFMKRHVSYAPLRPFIGGFIVAL------AVWAVGTHRYIGLGIP 285 Query: 301 -LVNSLLHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQA 359 +V + W + K++ T ++GSG GG TP ++GA +G A Sbjct: 286 VIVEAFQQPLAPWD-----FLGKMVFTVTSLGSGFKGGEVTPLFYIGATLG-------NA 333 Query: 360 VLPVHISQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVA 418 + P+ A +G A A A+ PL + +M E+ Q+ L+CVVAY+ + Sbjct: 334 LAPLLHLPFPLLAGIGFVAVFAGAANTPLASTIMAIEL-FGPQIGPFAALACVVAYLFS 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,714,900 Number of extensions: 69417 Number of successful extensions: 304 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 4 Length of query: 578 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 488 Effective length of database: 1,193,720 Effective search space: 582535360 Effective search space used: 582535360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)