BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0158.p (753 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetas... 1559 0.0 gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspi... 359 e-100 >gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 753 Score = 1559 bits (4036), Expect = 0.0, Method: Compositional matrix adjust. Identities = 753/753 (100%), Positives = 753/753 (100%) Query: 1 MSLTTTDSTLSATPSPKRAAGSGRTAAPTEVAPAAHSGVATFAHLIPKLEEYLTPSELKK 60 MSLTTTDSTLSATPSPKRAAGSGRTAAPTEVAPAAHSGVATFAHLIPKLEEYLTPSELKK Sbjct: 1 MSLTTTDSTLSATPSPKRAAGSGRTAAPTEVAPAAHSGVATFAHLIPKLEEYLTPSELKK 60 Query: 61 VKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMEDQGVKK 120 VKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMEDQGVKK Sbjct: 61 VKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDVMEDQGVKK 120 Query: 121 EELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKLADRLH 180 EELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKLADRLH Sbjct: 121 EELIERFGASVASLVDGLSKLDKIEFQSQVEAQAENFRKMLLAMARDVRVILVKLADRLH 180 Query: 181 NMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSFAHLHPLRHRTLSKAV 240 NMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSFAHLHPLRHRTLSKAV Sbjct: 181 NMRTLGSMPPEKKRRISRETMEVYVPIAHRLGLNNIYRELQELSFAHLHPLRHRTLSKAV 240 Query: 241 KAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVYG 300 KAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVYG Sbjct: 241 KAARGNRREVVTKIMESVTSTLIAAGIPAQVDGREKTLYGIYRKMRNKHLSFSQVLDVYG 300 Query: 301 FRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQI 360 FRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQI Sbjct: 301 FRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAIPKLNGYQSLHTTLIGPYGTPVEFQI 360 Query: 361 RTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKVD 420 RTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKVD Sbjct: 361 RTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTHAWLQSLLDIQKQTGDSAEFLEHVKVD 420 Query: 421 LFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGDI 480 LFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGDI Sbjct: 421 LFPDSVYVFTPKSKIIALPRGATALDFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGDI 480 Query: 481 VEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEPE 540 VEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEPE Sbjct: 481 VEIITSPTSRPSPNWLGYVRTGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEPE 540 Query: 541 LPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHMLP 600 LPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHMLP Sbjct: 541 LPSHIVERLLNESSAKTMDDLYAEIGIGTRMAPLVARHIMTMMDTGVSVPQLDPEGHMLP 600 Query: 601 NKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPDR 660 NKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPDR Sbjct: 601 NKPDPVVITGSEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPDR 660 Query: 661 WIELVWGQDLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYL 720 WIELVWGQDLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYL Sbjct: 661 WIELVWGQDLNRRFDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYL 720 Query: 721 RFTIQVEDRVHLARTMRGIRRIDSVARILRERG 753 RFTIQVEDRVHLARTMRGIRRIDSVARILRERG Sbjct: 721 RFTIQVEDRVHLARTMRGIRRIDSVARILRERG 753 >gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspirillum seropedicae SmR1] Length = 749 Score = 359 bits (921), Expect = e-100, Method: Compositional matrix adjust. Identities = 232/742 (31%), Positives = 362/742 (48%), Gaps = 54/742 (7%) Query: 53 LTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDV 112 L P + +V +A + ++ + SG+ + ++VA + +DA +AA + Sbjct: 19 LGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVLTALNVDAATRIAALAFEA 78 Query: 113 MEDQGVKKEELIERFGASVASLVDGLSKLDKI---EFQSQVEA------------QAENF 157 +E+L ERFG +A LV + +L + FQ Q E Q E Sbjct: 79 QMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQEVLRGKNAAQQAAAQIETL 138 Query: 158 RKMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKR--RISRETMEVYVPIAHRLGLNN 215 RKMLLAMA D+R +LV+LA R+ +R E + +R+T E+Y P+A+RLG+ Sbjct: 139 RKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYARQTFELYAPLANRLGIWQ 198 Query: 216 IYRELQELSFAHLHPLRHRTLSKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGRE 275 + EL++LSF L P+ ++ ++K ++ R R V + + + + A I A+V GR Sbjct: 199 LKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIARLRAEMAAQDIRAEVSGRP 258 Query: 276 KTLYGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAI 335 K +Y I+ KMR KH+ FS++ DV FRV+VD CY LG +H ++ P+P +F DYI+ Sbjct: 259 KHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGIVHDIWTPIPEEFDDYISR 318 Query: 336 PKLNGYQSLHTTLIGPYGTPVEFQIRTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTH- 394 PK NGYQSLHT + G +E QIRT EMH AE GVAAHW YK+ GS Q+ Sbjct: 319 PKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHWRYKESGGSNFSAQKYDEK 378 Query: 395 -AWLQSLLDIQKQTGDSA--------EFLEHVKVDLFPDSVYVFTPKSKIIALPRGATAL 445 AWL+ LL + + D+ +++E +K + +YV TP++++I LP GAT + Sbjct: 379 IAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIYVLTPQARVIELPSGATPI 438 Query: 446 DFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGDIVEIIT---SPTSRPSPNWLG--YVR 500 DFAY +H+D+G + A ++ PL L NG VEIIT P PS +WL Y Sbjct: 439 DFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVKSGPGVGPSRDWLSPDYAV 498 Query: 501 TGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEPELPSHIVERLLNESSAKTMDD 560 + + RS IR Q E+ +VGR LL L G + +E L + +DD Sbjct: 499 SSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREG----KTAVNLEELAQKLGFAKVDD 554 Query: 561 LYAEIGIGTRMAPLVARHIMTMMDTGV-SVPQLDPE---------GHMLPNKPDPVVITG 610 L + RH+ + G P++D E ++ V++ G Sbjct: 555 LCLSLA----KEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQGAKSGVLVVG 610 Query: 611 SEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPDRWIELVWGQDL 670 ++G QL+ CC P P D + G++ + + +H C + P+R I+ WG Sbjct: 611 TDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPERVIQTTWGAPA 670 Query: 671 NRR-FDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYLRFTIQVEDR 729 + I +L + +G L I+ N++ V+ G+A M FT ++ Sbjct: 671 RETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA---FTAEIGST 727 Query: 730 VHLARTMRGIRRIDSVARILRE 751 L + + IR + V R+ Sbjct: 728 EQLQKALTVIREVKGVIEAKRQ 749 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,282,121 Number of extensions: 96321 Number of successful extensions: 340 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 2 Length of query: 753 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 661 Effective length of database: 1,184,250 Effective search space: 782789250 Effective search space used: 782789250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)