BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0150.p (683 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311453|ref|YP_003775545.1| signal transduction containing ... 1400 0.0 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 423 e-119 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 423 e-119 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 405 e-114 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 379 e-106 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 1400 bits (3623), Expect = 0.0, Method: Compositional matrix adjust. Identities = 681/682 (99%), Positives = 682/682 (100%) Query: 1 MLSSVGTDDLACCSALLEAMQNLHGMALHAALGGIASGLLKRPGGRYVIGASAPVEGGLC 60 MLSSVGTDDLACCSALLEAMQNLHGMALHAALGGIASGLLKRPGGRYVIGASAPVEGGLC Sbjct: 1 MLSSVGTDDLACCSALLEAMQNLHGMALHAALGGIASGLLKRPGGRYVIGASAPVEGGLC 60 Query: 61 QPVQCEGALFGHFVFPPLTRPYSDQERQRLAMLAAFLGRLMQGRADTEQRVRALDQIEAK 120 QPVQCEGALFGHFVFPPLTRPYSDQERQRLAMLAAFLGRLMQGRADTEQRVRALDQIEAK Sbjct: 61 QPVQCEGALFGHFVFPPLTRPYSDQERQRLAMLAAFLGRLMQGRADTEQRVRALDQIEAK 120 Query: 121 LEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIGRNVLFLYESEDEEDSL 180 LEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIGRNVLFLYE+EDEEDSL Sbjct: 121 LEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIGRNVLFLYENEDEEDSL 180 Query: 181 HDLFLEHGGREMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLI 240 HDLFLEHGGREMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLI Sbjct: 181 HDLFLEHGGREMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLI 240 Query: 241 HHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDM 300 HHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDM Sbjct: 241 HHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDM 300 Query: 301 LLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAYVAQKLLALFDDPFFIDGHE 360 LLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAYVAQKLLALFDDPFFIDGHE Sbjct: 301 LLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAYVAQKLLALFDDPFFIDGHE 360 Query: 361 LRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLR 420 LRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLR Sbjct: 361 LRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLR 420 Query: 421 IETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETGL 480 IETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETGL Sbjct: 421 IETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETGL 480 Query: 481 IVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREFTRSLPDRVGAALERHMLSGEWL 540 IVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREFTRSLPDRVGAALERHMLSGEWL Sbjct: 481 IVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREFTRSLPDRVGAALERHMLSGEWL 540 Query: 541 ELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSFT 600 ELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSFT Sbjct: 541 ELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSFT 600 Query: 601 MGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVPA 660 MGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVPA Sbjct: 601 MGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVPA 660 Query: 661 PDFHAMLLEGRKLALESASASQ 682 PDFHAMLLEGRKLALESASASQ Sbjct: 661 PDFHAMLLEGRKLALESASASQ 682 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 423 bits (1088), Expect = e-119, Method: Compositional matrix adjust. Identities = 237/571 (41%), Positives = 333/571 (58%), Gaps = 10/571 (1%) Query: 106 DTEQRVRALDQIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAME--AI 163 D +R A ++ + I E V P G + N +++GY + E + Sbjct: 123 DVTERKEAAQALQEAERRYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLIV 182 Query: 164 G-RNVLF-LYESEDEEDSLHDLFLEHGG-REMEVRRRKKSGEVFWASLSLSVMRDQNGHP 220 G R++ LY + L E G E R ++ GE+ W S + + D+ G Sbjct: 183 GLRDISHQLYVEPERRQEFMRLMEEQGSVSNFESRVYRRDGEIIWISENARAVYDEAGKL 242 Query: 221 IGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMF 280 G + ITERK E + H A +DALTGLPNR +L + +AL AR + V F Sbjct: 243 SCYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAF 302 Query: 281 IDLNRFKPINDTLGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSP- 339 +DL++FK IND+LGH GD LL VA RL+ LRE D+VAR G DEF + L + Sbjct: 303 VDLDQFKFINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAG 362 Query: 340 -AYVAQKLLALFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGE 398 A V Q++LA P+ E +V ASIGVS YP + D E+LL+ AD AMYRAK+ G Sbjct: 363 IAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGR 422 Query: 399 NNEGGYACYSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRW 458 NN +A + + N L++L L A + +E LYYQPK+ +Q + GAEAL+RW Sbjct: 423 NNFQFFAPWMDSQVSNRLEML---INLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRW 479 Query: 459 QHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREF 518 + P++G++ P FIP AEE GLIV + +WVL CRQ +AW+ AGLPPI +A+N++ R+ Sbjct: 480 ESPQQGMVPPDRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQL 539 Query: 519 TRSLPDRVGAALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTG 578 +SLPD V L + L+ WLELEITE+++M ++ ++ + + LG+ +S+DDFGTG Sbjct: 540 NQSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTG 599 Query: 579 YSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQF 638 YSSLSYL+RFP++ LKID+SF I D + AI AII +A L +VIAEGVEDE+Q Sbjct: 600 YSSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQH 659 Query: 639 NFLREAGCNELQGYIFSRPVPAPDFHAMLLE 669 FL+E C+E+QGY F +P+ F A + E Sbjct: 660 AFLKENACDEVQGYYFGKPMEVAVFTAWMSE 690 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 423 bits (1087), Expect = e-119, Method: Compositional matrix adjust. Identities = 228/538 (42%), Positives = 328/538 (60%), Gaps = 8/538 (1%) Query: 139 DPAGFITSWNRGAEQLFGYSAMEAIGRNVLFLYESEDEEDSLHDLFL---EHGGREMEVR 195 +P I N ++ GYS+ EA+G+++ FL + + + +++ E + +R Sbjct: 411 EPDYTIEYVNPAFARITGYSSTEAVGKSIRFLEGRDSDPEGFETVWIAMREQREHNVVLR 470 Query: 196 RRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLIHHLAYYDALTGLPNR 255 K G W ++ +RD++G + L DIT K E + A D LTGL NR Sbjct: 471 NYHKDGSELWNDFYIAPVRDESGEVTHFVAALYDITSMKRYEAELEFQASRDTLTGLINR 530 Query: 256 TLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDMLLVEVAMRLRRALRE 315 +L ++QA+ A + V+F+DL+RFK +NDTLGH AGD LL VA RLR A+RE Sbjct: 531 HMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGDTLLRTVAGRLRAAVRE 590 Query: 316 QDVVARLGADEFAVALFGIDKDS-PAYVAQKLLALFDDPFFIDGHELRVGASIGVSLYPE 374 D VARLG DEF + L D+ + Q+++ P + GH L + S+GV+ YP Sbjct: 591 ADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPVTLGGHTLFMTCSVGVACYPN 650 Query: 375 NATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLRIETGLLHAFEYNEL 434 + TD ETL++ ADIAMYR KQ G NN + Y+ MN L+ LRIE L +A ++ E Sbjct: 651 DGTDGETLIKHADIAMYRGKQTGRNN---FQFYTPAMNEQALERLRIEGDLRNALDHGEF 707 Query: 435 LLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIPIAEETGLIVQLSDWVLDAVCR 494 LL+YQPKVD+ + + G+EAL+RWQHP+ G++ P FI +AEETGLI+ + WVL A C Sbjct: 708 LLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEETGLILPIGAWVLRAACT 767 Query: 495 QARAWKDAGLPPIRIALNVTAREF-TRSLPDRVGAALERHMLSGEWLELEITESMLMHST 553 Q R W+ G I +++N++ +F + L + LE L E LELE+TES++M Sbjct: 768 QCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLPPESLELELTESLMMTDV 827 Query: 554 DRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIA 613 + I I+ + LG+ +S+DDFGTGYSSL+YLKRFPI+ LKIDRSF I D +D AI Sbjct: 828 EHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAIT 887 Query: 614 SAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVPAPDFHAMLLEGR 671 ++II +A+ L+ +VIAEGVE ++Q ++LR GC+E+QGY+FS PVPA F +L + R Sbjct: 888 ASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQILRQDR 945 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 405 bits (1041), Expect = e-114, Method: Compositional matrix adjust. Identities = 215/440 (48%), Positives = 285/440 (64%), Gaps = 9/440 (2%) Query: 232 ERKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPIND 291 E + A++ I HLA+YD LTGLPNR L T+ V A+ A H N V+ +DL+RFK IND Sbjct: 658 ESRRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNIND 717 Query: 292 TLGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAY---VAQKLLA 348 T GH G+ +L E+A R R +LRE D++AR+G DEF V + D D P +AQKLL Sbjct: 718 TFGHETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLV--DDFDDPVQLGEIAQKLLT 775 Query: 349 LFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYS 408 PF IDG E + SIG++ YP + + +TL++ ADIAMYRAK G+++ Y +S Sbjct: 776 EARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDD---YRFFS 832 Query: 409 EEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLP 468 +E+N NT++ + +E L A E E +++YQPK+++ I GAEALVRWQHP RGLL P Sbjct: 833 DEINTNTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPP 892 Query: 469 GEFIPIAEETGLIVQLSDWVLDAVCRQARAWKDAGLPPIRIALNVTAREF-TRSLPDRVG 527 GEFI +AEET LI Q+ VLD CR + + RI++N+T +F L D + Sbjct: 893 GEFITLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIK 952 Query: 528 AALERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKR 587 ++RH LE EITESM+MH+ +R I+IM+ I G T+S+DDFGTGYSSL+YLKR Sbjct: 953 EVVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKR 1012 Query: 588 FPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCN 647 FP+NTLKID+SF IPD+ N AI AII ++ L KV+ EGVE EKQ LRE G + Sbjct: 1013 FPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSD 1072 Query: 648 ELQGYIFSRPVPAPDFHAML 667 E QGY FS+PVP F +L Sbjct: 1073 EYQGYYFSKPVPYAQFVQLL 1092 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 379 bits (973), Expect = e-106, Method: Compositional matrix adjust. Identities = 223/567 (39%), Positives = 326/567 (57%), Gaps = 20/567 (3%) Query: 116 QIEAKLEQQAQILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIGRNVLFLYESED 175 QIEA+L A + E ++ D I N+ ++ GYSA E +G++ L Sbjct: 469 QIEAELRISAVAFDS-REGMLITDANSKILRVNKAFTEITGYSAEEIVGKHPNLLRSDRH 527 Query: 176 EEDSLHDL---FLEHGGREMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITE 232 ++ G + E+ R+K+GEV+ L++S +++++ + +DI++ Sbjct: 528 GPAFFQEMRESIRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKDQQVTHYVSTHHDISD 587 Query: 233 RKNAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDT 292 RK AE+ I LA++DALT LPNRTLL + QA+ + ++FIDL+ FK +NDT Sbjct: 588 RKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKTLNDT 647 Query: 293 LGHVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDK------DSPAYVAQKL 346 +GH GD LL +VA RL +RE D VAR+G DEF V L G+++ + +K+ Sbjct: 648 IGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAIGEKI 707 Query: 347 LALFDDPFFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYAC 406 L + +D E R ASIG +++ A + L++ AD+AMY+AK+ G N Sbjct: 708 LTALGSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRN---ALRF 764 Query: 407 YSEEMNRNTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLL 466 + M L+ +E GL A + N+LLL+YQP+V ++ +TGAE LVRWQHP+RG++ Sbjct: 765 FDPAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTGAEVLVRWQHPQRGMV 823 Query: 467 LPGEFIPIAEETGLIVQLSDWVLDAVCRQARAWK-DAGLPPIRIALNVTAREFTRSLPDR 525 PG+FIP+AEETGLI+ L +WVL+ CRQ W + + IA+NV+A + PD Sbjct: 824 PPGDFIPLAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLRE--PDF 881 Query: 526 VGAAL---ERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSL 582 V L ER + L+LE+TES+L+ + + +I M + G+ SLDDFGTGYSSL Sbjct: 882 VQTVLDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSL 941 Query: 583 SYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLR 642 SYLKR P+N LKID+SF + D ND +IA I+ +A+ L VIAEGVE + Q +FL Sbjct: 942 SYLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLA 1001 Query: 643 EAGCNELQGYIFSRPVPAPDFHAMLLE 669 +AGC+ QGY F RPV F E Sbjct: 1002 DAGCHAYQGYFFCRPVTVEAFEKFAQE 1028 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,112,985 Number of extensions: 90576 Number of successful extensions: 412 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 330 Number of HSP's successfully gapped: 45 Length of query: 683 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 591 Effective length of database: 1,184,250 Effective search space: 699891750 Effective search space used: 699891750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)