BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0145.p (297 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311458|ref|YP_003775550.1| LysR family transcription regul... 604 e-174 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 202 2e-53 gi|300311424|ref|YP_003775516.1| LysR family transcription regul... 201 4e-53 gi|300313922|ref|YP_003778014.1| LysR family transcription regul... 192 2e-50 gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 189 3e-49 >gi|300311458|ref|YP_003775550.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 303 Score = 604 bits (1558), Expect = e-174, Method: Compositional matrix adjust. Identities = 296/297 (99%), Positives = 296/297 (99%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL Sbjct: 7 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS Sbjct: 67 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILC SPAYIARQGAPEQPEDLS Sbjct: 127 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCTSPAYIARQGAPEQPEDLS 186 Query: 181 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 240 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG Sbjct: 187 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 246 Query: 241 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFPHPENDPWLS 297 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFPHPENDPWLS Sbjct: 247 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARFPHPENDPWLS 303 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 202 bits (515), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 106/287 (36%), Positives = 176/287 (61%), Gaps = 1/287 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 + F V + GSFV+AAE L IS A +R + +LEA LG+RL+ R+TRRLSLTEAG YL Sbjct: 7 IECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTEAGALYL 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 ER + I+A +++A+ L+S + +P G L I FG L + + + +PE++LDI+ Sbjct: 67 ERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPEISLDIT 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 LS+R +D+++EG D+ + + D++++ R+L + +CASPAY+ G P P +L+ Sbjct: 127 LSDRIVDIIDEGYDMAIRIA-RLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAHPHELA 185 Query: 181 RHNCLNFSHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSLG 240 +H +++S+ + W N G + V + S++ N+G AL G+GI +P++ + Sbjct: 186 QHQTISYSYNQSKDEWQFNGPEGPVSVRVSSRLHVNNGDSCVQAALGGIGITRQPTFMID 245 Query: 241 DDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARF 287 LRSG+LV +L + ++ + VYPSR L AK+R+ +F++ F Sbjct: 246 QYLRSGQLVSILGDYGAPELGIYAVYPSRAHLPAKMRAMLDFLTEAF 292 >gi|300311424|ref|YP_003775516.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 201 bits (512), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 115/284 (40%), Positives = 174/284 (61%), Gaps = 1/284 (0%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 MRVFA+VVE GSF +AA+ + ++ A +R +ADLE HL TRL+NR+TRR++LTEAG+ YL Sbjct: 8 MRVFARVVEAGSFTQAAQQMGLTTAHVSRAVADLERHLRTRLMNRTTRRIALTEAGERYL 67 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 R + I++ VD+AEA S +P+G L I+ FGQ + +S Y + YP+VA++++ Sbjct: 68 LRCQQIMSYVDEAEAEASAAYVKPSGRLRIHAMTSFGQRYVIPSISGYHKRYPDVAVELT 127 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 L++R D++EEG D+ + + D+ +I RQLG +LCASPAY+ + GAP+QP DL Sbjct: 128 LAQRIPDMLEEGYDVSLVLARELPDSGLIYRQLGSTFSMLCASPAYLDKYGAPQQPADLL 187 Query: 181 RHNCLNF-SHEYLRHHWHVNTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSL 239 H CL S W + G V + S N + GMGI + P YS Sbjct: 188 NHACLQLVSPISPLDEWILEGPEGGTVVAVKSNFQVNVAEAMLTAIREGMGIGVLPIYSA 247 Query: 240 GDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFI 283 D LR+G +V+VLP + + + +YPSR+ L AK+ ++ +++ Sbjct: 248 VDALRAGTIVRVLPQHRLQNMGVYALYPSRQYLDAKISTWVDWM 291 >gi|300313922|ref|YP_003778014.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 192 bits (489), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 112/300 (37%), Positives = 173/300 (57%), Gaps = 16/300 (5%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 M+VF VV+ GS AAE LD+S V +R++A+LE +G RLL+R+TRRLSLT AG L Sbjct: 7 MQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTPAGNELL 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 R R +L +D + + P G L I + F Q+QL + + Y + YP VA+D+ Sbjct: 67 PRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPGVAVDLL 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 +R+++LVEE ID+ + + + ++I R LGV R I+CA+P Y+AR G P + EDL+ Sbjct: 127 ALDRTVNLVEERIDLAIRMT-NRLEPNLIARPLGVCRSIICATPDYLARHGLPRKVEDLA 185 Query: 181 RHNCLNFSHEYL-RHHWHVN------------TNGGVLDVPIVSKVVSNSGVLLRDLALA 227 HNCL +H Y+ R W + + V + + +N +L LA Sbjct: 186 LHNCL--THSYVGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQAVLA 243 Query: 228 GMGIVLRPSYSLGDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAEFISARF 287 G+GI P+Y++ + SG+LVQ+LP ++ Q+ M VY SR+ + A +R+ +F++ RF Sbjct: 244 GVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLDFLAQRF 303 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 189 bits (480), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 103/297 (34%), Positives = 170/297 (57%), Gaps = 6/297 (2%) Query: 1 MRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTEAGQAYL 60 M+ F VVE GSFV A ++ S +R +++LE LG RLL+R+TRRLSLT+ G+ Y Sbjct: 7 MQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTDEGRVYF 66 Query: 61 ERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPEVALDIS 120 R + +L + D E+ V L + + G L I FG QL + +++ +P + L+I+ Sbjct: 67 ARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPLITLNIT 126 Query: 121 LSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCASPAYIARQGAPEQPEDLS 180 LS+R+IDL+EEG D+ S D+ M+ R L R+I CA+P ++AR G P P +L Sbjct: 127 LSDRTIDLLEEGFDMAIRISALG-DSRMVARPLAPVRMIACAAPEFLARHGTPGTPAELR 185 Query: 181 RHNCLNFSHEYLRHHWHV-NTNGGVLDVPIVSKVVSNSGVLLRDLALAGMGIVLRPSYSL 239 + +++S+ W + +G V + S V N+G R +A+AG GI ++P + + Sbjct: 186 DYPFISYSYRAGGDDWSFRHVHGDTCQVRVRSTVYVNNGATCRAMAIAGQGIAIQPDFIV 245 Query: 240 GDDLRSGRLVQVLPGFDMG-QVIMSMVYPSRRLLSAKVRSFAEFISARFPHPENDPW 295 +++G+LV++ G+ G Q+++ +YP+R L KV+ +F+ +F P PW Sbjct: 246 NAAIQAGQLVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLIEQFRVP---PW 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 852,802 Number of extensions: 34592 Number of successful extensions: 248 Number of sequences better than 1.0e-08: 54 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 54 Length of query: 297 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 212 Effective length of database: 1,217,395 Effective search space: 258087740 Effective search space used: 258087740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)