BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0138.p
         (423 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311464|ref|YP_003775556.1| major facilitator superfamily p...   852   0.0  
gi|300312599|ref|YP_003776691.1| major facilitator superfamily p...    62   9e-11

>gi|300311464|ref|YP_003775556.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 423

 Score =  852 bits (2202), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 423/423 (100%), Positives = 423/423 (100%)

Query: 1   MNRGFYTIMAAQFFSSLADNALLIAAIALLAEMHSPAWMTPLLKLFFVLSYVLLAAFVGA 60
           MNRGFYTIMAAQFFSSLADNALLIAAIALLAEMHSPAWMTPLLKLFFVLSYVLLAAFVGA
Sbjct: 1   MNRGFYTIMAAQFFSSLADNALLIAAIALLAEMHSPAWMTPLLKLFFVLSYVLLAAFVGA 60

Query: 61  FADAFPKGKVMLITNMIKIVGCSLMFFTVHPLLAYAVVGFGAAAYSPAKYGILTELLPPE 120
           FADAFPKGKVMLITNMIKIVGCSLMFFTVHPLLAYAVVGFGAAAYSPAKYGILTELLPPE
Sbjct: 61  FADAFPKGKVMLITNMIKIVGCSLMFFTVHPLLAYAVVGFGAAAYSPAKYGILTELLPPE 120

Query: 121 KLVAANGWIEGLTVGSIIMGTVLGGALVNPKISSVILSFDFPLVDLPIDTPTEAALCIIS 180
           KLVAANGWIEGLTVGSIIMGTVLGGALVNPKISSVILSFDFPLVDLPIDTPTEAALCIIS
Sbjct: 121 KLVAANGWIEGLTVGSIIMGTVLGGALVNPKISSVILSFDFPLVDLPIDTPTEAALCIIS 180

Query: 181 LIYVIAALFNFNIPDTGARYEHQERNPIRLIVDFAGCFTTLWKDKLGQISLAVTTLFWGA 240
           LIYVIAALFNFNIPDTGARYEHQERNPIRLIVDFAGCFTTLWKDKLGQISLAVTTLFWGA
Sbjct: 181 LIYVIAALFNFNIPDTGARYEHQERNPIRLIVDFAGCFTTLWKDKLGQISLAVTTLFWGA 240

Query: 241 GATLQFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAMAAARFVPLKKSLTVMPLGIVM 300
           GATLQFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAMAAARFVPLKKSLTVMPLGIVM
Sbjct: 241 GATLQFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAMAAARFVPLKKSLTVMPLGIVM 300

Query: 301 GIVVMLMTTTTSVWVAYPLLVIVGALSGYFVVPMNALLQHRGHVLMSAGHSIAVQNFNEN 360
           GIVVMLMTTTTSVWVAYPLLVIVGALSGYFVVPMNALLQHRGHVLMSAGHSIAVQNFNEN
Sbjct: 301 GIVVMLMTTTTSVWVAYPLLVIVGALSGYFVVPMNALLQHRGHVLMSAGHSIAVQNFNEN 360

Query: 361 LSVLTMLVLYAFMVRMNLDVNTVIIIFGSFVAGIMYLIMRRHAANQREHDSLALIGEDKH 420
           LSVLTMLVLYAFMVRMNLDVNTVIIIFGSFVAGIMYLIMRRHAANQREHDSLALIGEDKH
Sbjct: 361 LSVLTMLVLYAFMVRMNLDVNTVIIIFGSFVAGIMYLIMRRHAANQREHDSLALIGEDKH 420

Query: 421 GHH 423
           GHH
Sbjct: 421 GHH 423


>gi|300312599|ref|YP_003776691.1| major facilitator superfamily
           permease [Herbaspirillum seropedicae SmR1]
          Length = 629

 Score = 62.0 bits (149), Expect = 9e-11,   Method: Compositional matrix adjust.
 Identities = 95/377 (25%), Positives = 149/377 (39%), Gaps = 60/377 (15%)

Query: 2   NRGFYTIMAAQFFSSLADNALLIAAIALLAEMH------SPAWMTPLLKLFFVLSYVLLA 55
            R F      QF  +L DN    A + +L           P  +  L+   F+L + L +
Sbjct: 12  QRRFAPFFWTQFLGALNDNVFKTALLTILTYDALSWTDLDPGLLNNLIPGLFILPFFLFS 71

Query: 56  AFVGAFADAFPKGKVMLITNM--IKIVGCSLMFFTVHPL--LAYAVVGFG--AAAYSPAK 109
           A  G  AD   KG+V     +  I I+G +   +  H L  L  AV+G G  +  + P K
Sbjct: 72  ATSGQLADKLEKGRVARFVKLLEIAIMGIAAYGWMTHHLWLLVAAVIGMGVHSTLFGPVK 131

Query: 110 YGILTELLPPEKLVAANGWIEGLTVGSIIMGTVLGGALVNPKISSVILSFDFPLVDLPID 169
           Y  L + L  E+L+  NG IE  T   I++G +LG  LV               V  P  
Sbjct: 132 YAYLPQQLRREELIGGNGLIEMGTFVGILLGEILGAVLV---------------VHKPWG 176

Query: 170 TPTEAALCIISLIYVIAALFNFNIPDTGARYEHQERNPIRLIVDFAGCFTTLWKDKLGQ- 228
               A + I   I V+  L +  IP + A     + N       F      L   +  + 
Sbjct: 177 LHLVAGMTIG--IAVLGWLASLRIPLSPAPVPELKVN----WNPFTETVRNLAYSRRNRP 230

Query: 229 --ISLAVTTLFWGAGATL--QFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAMAAARF 284
             +SL   + FW  GA +  QF V  +A+  LH       +L  + +VG+  G++   R 
Sbjct: 231 VFLSLLGNSWFWFYGAIMLAQFPV--YAKNYLHGDHSVFVLLLAVFSVGIGAGSLLCERL 288

Query: 285 VPLKKSLTVMPLG----IVMGIVVMLMT----TTTSV------------WVAYPLLVIVG 324
              K  + ++P G     V G+ +   +    T T++            W      + +G
Sbjct: 289 SGHKVEIGLVPFGSIGLSVFGLELYFASQAYVTPTAMLDLGGFLAQGGSWRILIDCLGIG 348

Query: 325 ALSGYFVVPMNALLQHR 341
              G ++VP+ AL+Q R
Sbjct: 349 VFGGLYIVPLFALIQTR 365


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.328    0.140    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,129,285
Number of extensions: 41126
Number of successful extensions: 204
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 204
Number of HSP's successfully gapped: 2
Length of query: 423
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 335
Effective length of database: 1,203,190
Effective search space: 403068650
Effective search space used: 403068650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 132 (55.5 bits)